General information for g10011.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g10011 is located on chr_1: 6604826..6607017 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10011 | g10011.t1 | isoform | g10011.t1 | 6604826 | 6607017 |
| chr_1 | g10011 | g10011.t1 | exon | g10011.t1.exon1 | 6604826 | 6606165 |
| chr_1 | g10011 | g10011.t1 | cds | g10011.t1.CDS1 | 6604826 | 6606165 |
| chr_1 | g10011 | g10011.t1 | exon | g10011.t1.exon2 | 6606215 | 6606300 |
| chr_1 | g10011 | g10011.t1 | cds | g10011.t1.CDS2 | 6606215 | 6606300 |
| chr_1 | g10011 | g10011.t1 | exon | g10011.t1.exon3 | 6606356 | 6606382 |
| chr_1 | g10011 | g10011.t1 | cds | g10011.t1.CDS3 | 6606356 | 6606382 |
| chr_1 | g10011 | g10011.t1 | exon | g10011.t1.exon4 | 6606431 | 6606581 |
| chr_1 | g10011 | g10011.t1 | cds | g10011.t1.CDS4 | 6606431 | 6606581 |
| chr_1 | g10011 | g10011.t1 | exon | g10011.t1.exon5 | 6606837 | 6607017 |
| chr_1 | g10011 | g10011.t1 | cds | g10011.t1.CDS5 | 6606837 | 6607017 |
| chr_1 | g10011 | g10011.t1 | TSS | g10011.t1 | NA | NA |
| chr_1 | g10011 | g10011.t1 | TTS | g10011.t1 | NA | NA |
Sequences
>g10011.t1 Gene=g10011 Length=1785
ATGTTTGCAAATAAAAAGAGCAATTCAATTCAGGCAGTTGATTTGTTTGTAGATAAAAAT
AGTGGGATAGTGACAAGAATATTTTACGCAATTTCATTCATTTTTACAACAGCTGCCTTC
TTTTTCTACGTCTATCGTTCTTATGAAAAGTGGAAAATTGAACCTGATATTGCTGTGAGA
CAAAATCAAATTTTTGTTAGTGAAATTCCTTTTCCTGCTGTTACAATCTGTTCTCCTGTA
TTTGCACGATCAGAAATTTTGGCTTATAAAAATTATTCATCAACTAATATTGCAATCCAT
CCTGAAGTTCTCGATAATTTAAATTCAACTATCGCTAATTATTTGATAGCAAATCAACAT
GCATGCAATCCATATTATGTAGCTGTGAGTGAAAAAATTGAGAAACTTCGAACAGAAAGG
AACATTGTGAAATTATTAAATGAAAGTTTCTTAAATGTTGATGAAGTTCTGAGCACATGT
GCTTTTGGATCACGACTTATAAATTGCAAGCAAATTTTTAATCGAGTTCTAACTGATCGT
GGTTTTTGTTATTCATTTAATATGCAAGGTTTTAACACAATTTTTAATTCTGATGTCATC
AGTGAAGATTTCAATTCATACAAGAGAACAAAAATTAATAAATCACCAAAATATAAATAC
TCATCTGAAGGTTTTGTTGATGATGACAATGAAACTATCGATTGGACATTAGATAAAGGT
TTTAATGAAGGACATCATATAGATTTCTTTCCTGTGTCAGCTAAAAAAGGCGAAACATTT
CTGGTCTTTCTCTATCTCGATAAATCTAATTTTGAAAATACTTGTGGCATTTTTACTAGT
GTTTTTAATTATTATCTTCACTTGCCTAACGAAATGCCAAGTCAACTCCATAGAAAACAA
TTCGTCAGATTTCATGAGAAAACTTATGTCTCATTGACTGCAACAGTTTATAAAACACAT
AAAGATTTACAAAAACTTGAACCTGATGCTCGTGGTTGCTACTTTGAAGGTGAAAGAAAA
TTGAAGTATTTCAAATCTTACACAAAAAATAATTGCAATCTTGAATGCATCGCAAATTTC
ACACTAAAAAATTGCGGATGCTTGAAATTTTCAATGCCACGCGATAAAGACATACCAGTT
TGTGATTTTGATAAAAGTAAATGTTATTATAGTGCAATGAATGTTTGGCCAGGATATAAT
TTGACAACAAAAGAAAGTGTAGGATCTTGTGGATGTTTAAATACGTGCAATGATATAAAA
TATAGAATTGCAATTGAAGAAGGCTCAAGAACAGAATTAAAAGAACATCCAATTTTGATG
GATAAAGGAATCACTGAAGAAGACATTTTTGCATATTTGAAATTCACATTTGATGAAACT
TTTATAGTTGAAGAAGAAAGTTATGCACCTTATGCTTTACAAAATTTTATTGCTGACACT
GGCGGTCTTTTGGGACTGTTTATTGGATGCTCATTATTGTCATTCTTTGAGCTTTTCTAC
TTCATCTCAATGAAAATTTCACAAGCTTTTAATAAAGGGAAAAAGTTAGAAAGTTTGCAA
AAAGTAGCTCATGAAAATTTTCATGTGAATGTCATCAAAGTAAGACCAATTGGAAAAGAA
CAAATTAATGTCCTGAAGGAAAATGATGTTTGCACAAAAGAAGAATTACAAAAATACCGA
CAACAGAATGATCTACAAATTTATGAATTAAAGAATTTATTGAAAAAACGCGACAAAGAG
CTGGCCTGCATTTTAAATCAAATGTTTTCACAATATAGAGAATGA
>g10011.t1 Gene=g10011 Length=594
MFANKKSNSIQAVDLFVDKNSGIVTRIFYAISFIFTTAAFFFYVYRSYEKWKIEPDIAVR
QNQIFVSEIPFPAVTICSPVFARSEILAYKNYSSTNIAIHPEVLDNLNSTIANYLIANQH
ACNPYYVAVSEKIEKLRTERNIVKLLNESFLNVDEVLSTCAFGSRLINCKQIFNRVLTDR
GFCYSFNMQGFNTIFNSDVISEDFNSYKRTKINKSPKYKYSSEGFVDDDNETIDWTLDKG
FNEGHHIDFFPVSAKKGETFLVFLYLDKSNFENTCGIFTSVFNYYLHLPNEMPSQLHRKQ
FVRFHEKTYVSLTATVYKTHKDLQKLEPDARGCYFEGERKLKYFKSYTKNNCNLECIANF
TLKNCGCLKFSMPRDKDIPVCDFDKSKCYYSAMNVWPGYNLTTKESVGSCGCLNTCNDIK
YRIAIEEGSRTELKEHPILMDKGITEEDIFAYLKFTFDETFIVEEESYAPYALQNFIADT
GGLLGLFIGCSLLSFFELFYFISMKISQAFNKGKKLESLQKVAHENFHVNVIKVRPIGKE
QINVLKENDVCTKEELQKYRQQNDLQIYELKNLLKKRDKELACILNQMFSQYRE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g10011.t1 | Gene3D | G3DSA:1.10.287.820 | - | 328 | 416 | 7.5E-9 |
| 11 | g10011.t1 | Gene3D | G3DSA:1.10.287.770 | - | 454 | 508 | 1.3E-8 |
| 2 | g10011.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 19 | 521 | 5.1E-46 |
| 3 | g10011.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 19 | 521 | 5.1E-46 |
| 5 | g10011.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 67 | 83 | 9.5E-6 |
| 6 | g10011.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 347 | 358 | 9.5E-6 |
| 4 | g10011.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 358 | 375 | 9.5E-6 |
| 7 | g10011.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 481 | 497 | 9.5E-6 |
| 1 | g10011.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 9 | 501 | 1.2E-61 |
| 12 | g10011.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 26 | - |
| 16 | g10011.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 27 | 45 | - |
| 14 | g10011.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 46 | 482 | - |
| 15 | g10011.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 483 | 502 | - |
| 13 | g10011.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 503 | 594 | - |
| 9 | g10011.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 23 | 45 | - |
| 8 | g10011.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 480 | 502 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed