General information for g10012.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g10012 is located on chr_1: 6607200..6609167 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10012 | g10012.t1 | isoform | g10012.t1 | 6607200 | 6609167 |
| chr_1 | g10012 | g10012.t1 | exon | g10012.t1.exon1 | 6607200 | 6608551 |
| chr_1 | g10012 | g10012.t1 | cds | g10012.t1.CDS1 | 6607200 | 6608551 |
| chr_1 | g10012 | g10012.t1 | exon | g10012.t1.exon2 | 6608607 | 6608692 |
| chr_1 | g10012 | g10012.t1 | cds | g10012.t1.CDS2 | 6608607 | 6608692 |
| chr_1 | g10012 | g10012.t1 | exon | g10012.t1.exon3 | 6608743 | 6608905 |
| chr_1 | g10012 | g10012.t1 | cds | g10012.t1.CDS3 | 6608743 | 6608905 |
| chr_1 | g10012 | g10012.t1 | exon | g10012.t1.exon4 | 6609014 | 6609167 |
| chr_1 | g10012 | g10012.t1 | cds | g10012.t1.CDS4 | 6609014 | 6609167 |
| chr_1 | g10012 | g10012.t1 | TSS | g10012.t1 | NA | NA |
| chr_1 | g10012 | g10012.t1 | TTS | g10012.t1 | NA | NA |
Sequences
>g10012.t1 Gene=g10012 Length=1755
ATGTTGAATTTAGCGAAAATAAATCAAGAAAAGTTGAATTTCAGAGATAATTTGCAGTCA
AATAGAACTTATAACATAGCAAGTCGAATTTTCTACACATTTTCTTTTATATGTACAACT
TTAGCTTTCTTATTCTACGTTTATCGTGCTTATAAAAAATGGAAAATTATTCCTGACATT
GTTATAAGAAAGAATCAAAAGTTTCTTGGTGAAATTCCATTTCCAGCTGTGACAATTTGT
TCACCTGTTTTCTCACGACCAGAACTTTTGTATTATCCCAACTATTCATCTGACACTCTC
AAATCCAATATAAAAGTCCTTGCAAAAATAACGCCAAATGAAGCAAATTATTACATTTCT
AACATGCAAGCATGCAATCCAAGTTACACGGCAGTCATTGAGGTTATAAAAACTATTCAA
ACTAGAAATAATACTGTTGAACTTTTAAATGAAAGTTTTTTGTCTGTTGATGAAGTTTTC
GCTATGTGTCATTATGCAGCAAAATTGACTGAATGTAAAAGACTTTTTAATCGTGTTCTA
ACTGATCGTGGATTTTGTTATTCATTTAATATGCAAGGATTTAATACCATTTTTAATGCT
GATGTCATAAGTGATGATTTTAATTCATATAAAAGAAGAAAAATATCAAAATCACCATAC
AAACATCATGATTTACATTCTGAACGAATTAATGATGACAATGAAACTGTAGAATGGACA
CTTGATAAAGGTTTTATTGAAGATCATGACAATGACATCATACCAGTTGCTGCTGATAAA
AATACTAATCTTGGGATTTATTTATTTGTTGATAAAAATAATTTGGAAAATTCTTGTACA
AAATATGCAAACACATTTAATTATTATTTTCATTTACCTAATGAAATTTCTACACAAATG
CATAAAAAACATTTCATACCAATTAATGAGAAAAATATTGTGACTTTAACAGCAAATGTT
GTTAAAACAAGCAATGAATTAAGAAAATTGAAACCAATTGCTCGAGGATGTTACTTTGAA
GGTGAAAGAAAATTGAAATTTTTTAATTCTTACACAAAATTAAATTGTGAATTTGAATGT
ATGACAAATTACACACTAAAAATTTGCGGTTGTTTAAAATTTTCAATGCCACGTGATAAA
AATACACCGATTTGTAATATTGATAAAATTCTTTGCTATTTCGATGCTATGTCGTATTGG
CCAGGTTATAATTTGACAACAAAACAAATAGAAGCGTCATGTGGTTGTTTAGATACGTGC
AGTGACATAAAATATAGCATTTTATCGATTGAAACCACACCAATGTCAATGAATAAGAGC
AAATTTTTAATGAAAAATGGCTTTGAACAAGAAGATGCACTGTCATATTTGGCTTTCATT
TTTGAAAACATTTTTATTACTGAAGAAGAAAGTTATGCACCATATGCTATTCAAAATTTC
ATTGCAGACATTGGTGGACTTTTAGGACTTTTTATTGGTTGCTCAATTTTGTCAATTTTT
GAGTTTGTTTTTTACATTGTTTATAAAACTACAGATAATATTAAAATTTTTAAAGACAAA
ATGAAATTTGTTGATGATAATAAAGGTATCATAATTGTAAAACCATTTCAAAATCATACT
GATTCAGTTATGCATGAAAAACTCCAAAATGAACATATTGATAAGTTTATTCAACAACTA
CGACAAGAATTCGAAATAAAACTGCATGAAAATCAACTTGAAATTCTTAAAATATTGAAT
GAAAAACTCAACTGA
>g10012.t1 Gene=g10012 Length=584
MLNLAKINQEKLNFRDNLQSNRTYNIASRIFYTFSFICTTLAFLFYVYRAYKKWKIIPDI
VIRKNQKFLGEIPFPAVTICSPVFSRPELLYYPNYSSDTLKSNIKVLAKITPNEANYYIS
NMQACNPSYTAVIEVIKTIQTRNNTVELLNESFLSVDEVFAMCHYAAKLTECKRLFNRVL
TDRGFCYSFNMQGFNTIFNADVISDDFNSYKRRKISKSPYKHHDLHSERINDDNETVEWT
LDKGFIEDHDNDIIPVAADKNTNLGIYLFVDKNNLENSCTKYANTFNYYFHLPNEISTQM
HKKHFIPINEKNIVTLTANVVKTSNELRKLKPIARGCYFEGERKLKFFNSYTKLNCEFEC
MTNYTLKICGCLKFSMPRDKNTPICNIDKILCYFDAMSYWPGYNLTTKQIEASCGCLDTC
SDIKYSILSIETTPMSMNKSKFLMKNGFEQEDALSYLAFIFENIFITEEESYAPYAIQNF
IADIGGLLGLFIGCSILSIFEFVFYIVYKTTDNIKIFKDKMKFVDDNKGIIIVKPFQNHT
DSVMHEKLQNEHIDKFIQQLRQEFEIKLHENQLEILKILNEKLN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g10012.t1 | Coils | Coil | Coil | 566 | 584 | - |
| 15 | g10012.t1 | Gene3D | G3DSA:1.10.287.770 | - | 29 | 58 | 5.1E-10 |
| 11 | g10012.t1 | Gene3D | G3DSA:2.60.470.10 | - | 60 | 192 | 5.1E-10 |
| 12 | g10012.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 83 | 154 | 5.1E-10 |
| 13 | g10012.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 276 | 285 | 3.1E-32 |
| 10 | g10012.t1 | Gene3D | G3DSA:2.60.470.10 | - | 286 | 473 | 3.1E-32 |
| 14 | g10012.t1 | Gene3D | G3DSA:1.10.287.820 | - | 329 | 420 | 3.1E-32 |
| 16 | g10012.t1 | Gene3D | G3DSA:1.10.287.770 | - | 474 | 503 | 3.1E-32 |
| 2 | g10012.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 23 | 527 | 2.9E-45 |
| 3 | g10012.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 23 | 527 | 2.9E-45 |
| 5 | g10012.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 70 | 86 | 2.2E-6 |
| 6 | g10012.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 362 | 379 | 2.2E-6 |
| 7 | g10012.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 471 | 485 | 2.2E-6 |
| 4 | g10012.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 485 | 501 | 2.2E-6 |
| 1 | g10012.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 23 | 505 | 4.5E-60 |
| 20 | g10012.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 29 | - |
| 22 | g10012.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 48 | - |
| 19 | g10012.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 49 | 67 | - |
| 24 | g10012.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 68 | 85 | - |
| 21 | g10012.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 86 | 483 | - |
| 23 | g10012.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 484 | 508 | - |
| 18 | g10012.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 509 | 584 | - |
| 9 | g10012.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 26 | 48 | - |
| 8 | g10012.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 486 | 508 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed