General information for g10074.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g10074 is located on chr_1: 7262299..7264184 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10074 | g10074.t1 | isoform | g10074.t1 | 7262299 | 7264184 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon1 | 7262299 | 7262490 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS1 | 7262299 | 7262490 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon2 | 7262542 | 7262856 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS2 | 7262542 | 7262856 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon3 | 7262910 | 7263129 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS3 | 7262910 | 7263129 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon4 | 7263188 | 7263292 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS4 | 7263188 | 7263292 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon5 | 7263346 | 7263415 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS5 | 7263346 | 7263415 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon6 | 7263474 | 7263607 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS6 | 7263474 | 7263607 |
| chr_1 | g10074 | g10074.t1 | exon | g10074.t1.exon7 | 7263670 | 7264184 |
| chr_1 | g10074 | g10074.t1 | cds | g10074.t1.CDS7 | 7263670 | 7264184 |
| chr_1 | g10074 | g10074.t1 | TTS | g10074.t1 | 7264352 | 7264352 |
| chr_1 | g10074 | g10074.t1 | TSS | g10074.t1 | NA | NA |
Sequences
>g10074.t1 Gene=g10074 Length=1551
ATGGATGAAGATCGAATTGCACCAATTTTTAAAGGTCGACATATTTTATTGACGGGCTCT
ACAGGTTTTATGGGAAAAGTATTTGTCGAGAAGTTATTACGTATTACAGAAGTAGATAAA
ATTTATCTTTTGATGCGTGTAAAGAAAGGAAAAAATCCTAAGGATCGTCTTGTGGATATG
TTTAATAATCCAATATTTGATATTGTCAAGAAGCAAATGGGCATTGATGCAATGATTAAT
AAGATTGTTATTATAACTGGTGATTGTGCACTTGAAGGCTTGGGTATTTCTGAGGAGCAG
CGTCAACTCATCATTAATAATGTGACACTCATGTACCATTTTGCTGCAACTATTCGTTTT
GATGAAAAGCTCAAAAAAGCAGTTGAGCTTAACACAAGAGGAACACGTGAAATGATCAAA
CTCGGAAAAGAATGCAAAAAACTTGACATGTTTGGATATATGTCAACATCATATTGTCAT
TTGCATGAAAGCTTTTTGCTTGAAAAACCATACGATCCACCAGCAGATCCCAATAAAATT
ATAAACTCAATTGAAATTCTAACTGAAGAAGAAGTTGAAAAAATGGTTAAAAAAATTCTG
GGTAATTTACCAAATTCTTATGCTTTCACAAAAGCTCTTGCTGAAGCACTCGTCAATGAT
GCATGCCAAAAAGAAAATTTACCAGCAATGATTTTGCGACCATCTATTGTTATTCCAACT
CTTGAAGATCCGATAAGAGGTTGGACCGATAATCTCAATGGACCTGCGGGAATGATGATA
GGCGCAGGTAAAGGTGTTATAAGAACAATTTATTGCGATCAAAAAAGTTATGGCGATTTT
CTTCCTGTTGATGTTGCTATTAATGGTTTGATGGTTTGCACATGGAATTACATTCAATTT
AATGATAAAGAACATTATATTGTCAATTTTACGAGCTCTCAGGATATTCAAGTAACATGG
GAAGCTATTCTTGAGATGGGTCAAGAAATTATTAATGAAAAATATCCATTGAACCAAATT
CTCTGGTATCCAGGAGGTGGAATGTCAAAATATCGTTGGGTTCATAAAATAAAAGCGTTC
TTATTTCATTGGATTCCTGCATTGCTTATTGATACATTGTTATTTTGCTTGGGTTTTAAA
CCAATTTTATGGCGTGTTCATGAAAGGATTGCCAAAGGAATGGAAGTACTTGAATATTAT
GCAAACAATCAATGGGACTTTGACAACAAGCATGCAATTATTATAAGAGCAAGAATGAAT
AAAACAGAATTGAAGAAATATAAAGTTGATGCAAAAGGAATAGATATTTATAAATATTTT
GAGGATTGCATTCTTGGTGGAAGAAGATATTTGCTTAAACAACCTGACAGTATGTTACCA
TCTGCAAGACGAATGATGAAAGTCATGTATGTAATTGATAGATTTTGTAAAACGATCATT
TATGGTGGTCTGCTATATTGGCTTATAAAACGATTGCTTCCGCTCTTTTTCACAAGAGAA
GCTCTTAATAATTTTTATTCATCAATTTTTAACACAGCAAATGTGTCATAA
>g10074.t1 Gene=g10074 Length=516
MDEDRIAPIFKGRHILLTGSTGFMGKVFVEKLLRITEVDKIYLLMRVKKGKNPKDRLVDM
FNNPIFDIVKKQMGIDAMINKIVIITGDCALEGLGISEEQRQLIINNVTLMYHFAATIRF
DEKLKKAVELNTRGTREMIKLGKECKKLDMFGYMSTSYCHLHESFLLEKPYDPPADPNKI
INSIEILTEEEVEKMVKKILGNLPNSYAFTKALAEALVNDACQKENLPAMILRPSIVIPT
LEDPIRGWTDNLNGPAGMMIGAGKGVIRTIYCDQKSYGDFLPVDVAINGLMVCTWNYIQF
NDKEHYIVNFTSSQDIQVTWEAILEMGQEIINEKYPLNQILWYPGGGMSKYRWVHKIKAF
LFHWIPALLIDTLLFCLGFKPILWRVHERIAKGMEVLEYYANNQWDFDNKHAIIIRARMN
KTELKKYKVDAKGIDIYKYFEDCILGGRRYLLKQPDSMLPSARRMMKVMYVIDRFCKTII
YGGLLYWLIKRLLPLFFTREALNNFYSSIFNTANVS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g10074.t1 | CDD | cd05236 | FAR-N_SDR_e | 13 | 326 | 6.3502E-109 |
| 15 | g10074.t1 | CDD | cd09071 | FAR_C | 361 | 452 | 5.09712E-31 |
| 8 | g10074.t1 | Gene3D | G3DSA:3.40.50.720 | - | 2 | 357 | 5.2E-67 |
| 3 | g10074.t1 | PANTHER | PTHR11011:SF61 | FATTY ACYL-COA REDUCTASE | 3 | 492 | 2.3E-215 |
| 4 | g10074.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 3 | 492 | 2.3E-215 |
| 1 | g10074.t1 | Pfam | PF07993 | Male sterility protein | 17 | 288 | 7.8E-71 |
| 2 | g10074.t1 | Pfam | PF03015 | Male sterility protein | 362 | 454 | 3.6E-26 |
| 10 | g10074.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 359 | - |
| 13 | g10074.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 360 | 379 | - |
| 9 | g10074.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 380 | 467 | - |
| 12 | g10074.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 468 | 489 | - |
| 11 | g10074.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 490 | 516 | - |
| 7 | g10074.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 13 | 359 | 1.32E-24 |
| 6 | g10074.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 362 | 384 | - |
| 5 | g10074.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 470 | 489 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13356 (alcohol-forming fatty acyl-CoA reductase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed