General information for g10191.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Nedd8-activating enzyme E1 regulatory subunit.
Parent gene
- The parent gene g10191 is located on chr_1: 8158117..8159746 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10191 | g10191.t1 | TTS | g10191.t1 | 8158078 | 8158078 |
| chr_1 | g10191 | g10191.t1 | isoform | g10191.t1 | 8158117 | 8159746 |
| chr_1 | g10191 | g10191.t1 | exon | g10191.t1.exon1 | 8158117 | 8159614 |
| chr_1 | g10191 | g10191.t1 | cds | g10191.t1.CDS1 | 8158117 | 8159614 |
| chr_1 | g10191 | g10191.t1 | exon | g10191.t1.exon2 | 8159685 | 8159746 |
| chr_1 | g10191 | g10191.t1 | cds | g10191.t1.CDS2 | 8159685 | 8159746 |
| chr_1 | g10191 | g10191.t1 | TSS | g10191.t1 | 8159838 | 8159838 |
Sequences
>g10191.t1 Gene=g10191 Length=1560
ATGTCATCATCTGTACCATCACCAGAAAGTGACAAAAGTCGCAAATATGATAGACAAATA
AGATTATGGGGCGATCATGGACAAAATTTATTGGAAAACTCTCAGATATGTTTAATTAAT
GCTAATGCATTAGGAACTGAAATTTTAAAAGGCCTTGCATTACCCGGAATAGGAAGTTTT
ACAATTATTGATCAAAATTTAGTAACAGAAGAGGATATTGGATCAAATTTCTTCCTTGAA
CAGTCTTCGCTTGGGAAATCACGTGCTAAATGTGCTACTCAATTTTTACAAGAGCTTAAT
CCAGATGTAAATGGAGAGTCAATTGATGAAAATGTTGATAACATTATTGCAAATAATTCA
GATTTCTTTCAAAACTTTTCTGTTGTTGTTGCCTGTGCACTAAATGAAAAATCCCTCATC
AAACTCTCTAATTTATTATGGGATCTTAATATTCCTCTCATTTCTTGTAAAACAGTTGGA
TTCATTGGCACAGCACGTATTCAATTAAAAGAATATTGTGTTATTGAAACACATCCTGAT
AATAAGCATCATGACTTGCGATTAGAAGAACCCTTTGATTCACTAAAGGAACATTGCGAA
AAAATTGAGCTTGGTTCGAAAGTTCCTTGGTTAGTTATTCTATTTAAACATCTTGAAGCT
TATAAAGTAAAATATAATAAAATTCCACAAACTTATAAAGAAAAATCAGAATTGAGAACA
ATGATTCGAGATAGCATGTCGGCGGATGAAGAAAATTTTGAAGAAGCTATAAAAGCTGTT
AACAGTAGTTTTGGTGGAGGAAAACCCAATTCAGTTCTTCAAGAAATACTTAATGATAAT
GCCTGTATAAACTTGACTAAAAATAGTCCGCCGTTTTGGATCATGGTTCGTGCATTAAAA
GATTTTATTGAAAATGAAGGAAAAGGCTATTTGCCGCTTCCAGGTATTATTCCGGATATG
ACTGCAGAGACATCATTATATATCAATCTTCAAAATGTTTATCGAGCAAAAGCTTTGCAA
GATGCTGATATAGTTTTTCGTCGAGTGCAGGAACTTTTGGAAGAGCTTAACAAATCATGT
GATTGGATCACTGAAAAGGATGTGAGGCTTTTTTGTCGTGAAGCTGCATATATTGCTATC
ATTCGTGGATCAAAAATTTCTGATGAATATGAAAAAGGATCGAACACAGCAACAATTGCA
AGCGAATTGGAAAATATTGATTCATTAATGGGTCATTATGTCGTACTAAGAAGTCAAGAT
CGATTTATTACAGAACATGGATGTATGCCAGGCGAAATTCAAGTGGATATTGACACAGCA
CGAGTCAAGAGTCAAGCATCAAAGCTTTTGAACGATTGGGGTATAAATATTTCACTCAAT
GATGATTTAATTCATGAAATTTGTCGCTATGGTGGTTCAGAAATACATTCTGTATCTGCA
TTTCTTGGTGGCGCGATTGCTCATGAAATAATAAAAATTGTTACACGTCAATATAAATCA
TTGGATAATACAATAATCTATGATGGCATTAATTCACAATGTGCAACATTTAAATTTTAA
>g10191.t1 Gene=g10191 Length=519
MSSSVPSPESDKSRKYDRQIRLWGDHGQNLLENSQICLINANALGTEILKGLALPGIGSF
TIIDQNLVTEEDIGSNFFLEQSSLGKSRAKCATQFLQELNPDVNGESIDENVDNIIANNS
DFFQNFSVVVACALNEKSLIKLSNLLWDLNIPLISCKTVGFIGTARIQLKEYCVIETHPD
NKHHDLRLEEPFDSLKEHCEKIELGSKVPWLVILFKHLEAYKVKYNKIPQTYKEKSELRT
MIRDSMSADEENFEEAIKAVNSSFGGGKPNSVLQEILNDNACINLTKNSPPFWIMVRALK
DFIENEGKGYLPLPGIIPDMTAETSLYINLQNVYRAKALQDADIVFRRVQELLEELNKSC
DWITEKDVRLFCREAAYIAIIRGSKISDEYEKGSNTATIASELENIDSLMGHYVVLRSQD
RFITEHGCMPGEIQVDIDTARVKSQASKLLNDWGINISLNDDLIHEICRYGGSEIHSVSA
FLGGAIAHEIIKIVTRQYKSLDNTIIYDGINSQCATFKF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10191.t1 | CDD | cd01493 | APPBP1_RUB | 14 | 517 | 0 |
| 6 | g10191.t1 | Gene3D | G3DSA:3.40.50.720 | - | 5 | 277 | 0 |
| 5 | g10191.t1 | Gene3D | G3DSA:3.40.50.720 | - | 363 | 519 | 0 |
| 2 | g10191.t1 | PANTHER | PTHR10953 | UBIQUITIN-ACTIVATING ENZYME E1 | 10 | 519 | 0 |
| 3 | g10191.t1 | PANTHER | PTHR10953:SF29 | NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT | 10 | 519 | 0 |
| 7 | g10191.t1 | PIRSF | PIRSF039099 | APP-BP1 | 6 | 519 | 0 |
| 1 | g10191.t1 | Pfam | PF00899 | ThiF family | 16 | 505 | 0 |
| 4 | g10191.t1 | SUPERFAMILY | SSF69572 | Activating enzymes of the ubiquitin-like proteins | 11 | 518 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008641 | ubiquitin-like modifier activating enzyme activity | MF |
| GO:0045116 | protein neddylation | BP |
| GO:0019781 | NEDD8 activating enzyme activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04532 (NEDD8-activating enzyme E1 regulatory subunit)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
