Gene loci information

Transcript annotation

  • This transcript has been annotated as Glutamate–cysteine ligase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10195 g10195.t1 TTS g10195.t1 8166281 8166281
chr_1 g10195 g10195.t1 isoform g10195.t1 8166649 8168817
chr_1 g10195 g10195.t1 exon g10195.t1.exon1 8166649 8166832
chr_1 g10195 g10195.t1 cds g10195.t1.CDS1 8166649 8166832
chr_1 g10195 g10195.t1 exon g10195.t1.exon2 8166901 8167752
chr_1 g10195 g10195.t1 cds g10195.t1.CDS2 8166901 8167752
chr_1 g10195 g10195.t1 exon g10195.t1.exon3 8167819 8168250
chr_1 g10195 g10195.t1 cds g10195.t1.CDS3 8167819 8168250
chr_1 g10195 g10195.t1 exon g10195.t1.exon4 8168308 8168614
chr_1 g10195 g10195.t1 cds g10195.t1.CDS4 8168308 8168614
chr_1 g10195 g10195.t1 exon g10195.t1.exon5 8168688 8168817
chr_1 g10195 g10195.t1 cds g10195.t1.CDS5 8168688 8168817
chr_1 g10195 g10195.t1 TSS g10195.t1 8169174 8169174

Sequences

>g10195.t1 Gene=g10195 Length=1905
ATGGGATTATTGACAGAAGGCAGTCCACTTTCATGGGAGGAGACAAAAAATTTGGCACAG
CATGTGAGAAAACATGGTATCAAGCAGTTTATTAACTTATTTGCAAGATTAAAAGACCGT
CAAGGTGATGTACTAAAATGGGGTGATGAAGTTGAGTATATGATTATAAGGTTTGATGAC
GATAAACAAATAGCACAAGCCTCTTTAAGAGCACTTGAAATACTGAACGTGTTACAAGAA
CCGGAACTGCGCGATCCAGATGGAGTAAAGTCACTTTGGCGCCCTGAATATGCGGCATAT
ATGGTCGAAGGTACACCTGGTAAACCCTATGGAGGGCTATTAGCTCACTTTAATGTTGTT
GAATCATCAATGAAATACCGTAGAGAAGAAGTGTCCAAGTTGTTAAAAGATGGTGAAGAA
GTTATGTCGATTACAAGTTTTCCACGTTTGGGTTGTCCACAATTTTCATATCCATCATAT
GATGTGCAACCAGACAATCCACAATCTGCAGCTCAATCATTATTCTTTCCTGATGAAGCT
ATATATTCGGGTCATCCACGTTTCAAAAATTTAACGCGAAATATTAGATTAAGACGAGGT
GAAAAGGTGTGCATCAACGTTCCAGTTTTTAAAGATGAAAACACAAAGTATCCTATTCCT
TATTCACTGCCTGAAACGCCTGATCATGTTTATATGGATGCGATGGGTTTCGGTATGGGA
AATTGTTGCTTGCAATTAACATTTCAAGCGTGTAATATCAATGAAGCTAGAGTTCTTTAT
GATCAATTGACTCCTCTCTGTCCAATTATGTTAGCATTGACTGCTGCATCTCCCTTATAT
AGGGGCTTTTTAACTGATATCGATTGCAGATGGAATGTTATTTCAGCATCTGTAGATTGT
AGAACAAAAGAGGAAAGAGGTCTTGAACCTCTAAAAGAAAATAAATTTAGAATCAACAAG
TCACGCTATGATTCAATCGATTCTTATCTATCTGAGGAGGGCGAAAAATACAACGACGTT
CCTTTACTTTACAATGAAGAAGACTATAAAACTTTACGTGAAGGTGGAATTGATCATTTA
TTGGCACAGCATATTGCCCATTTATTTATTCGGGATACTGTTTCTTTATTCAGTGAAAAA
ATTCATCAAAATGATGAAGAAGAGACCGATCATTTCGAAAACATTCAATCAACAAATTGG
CAAACAATGCGATTTAAACCACCACCACCTTCGAGTAGCATTGGCTGGCGAGTCGAATTC
CGTCCTTGTGAACTCCAATTAACGGATTTTGAGAATGCTGCAATTGTTGTTTTCATTGTA
TTGTTAACGCGTGTTATACTCTCATTCAAACTTAATTTCCTTATACCAATCAGTAAAGTT
GATGAGAATATGCAAAAGGCACAAAAACGAAATGCTTGTAAAGAAGAAAAATTTTGGTTC
CGAAGAAATTTCAACGGTAAAAAAGAAGACAATAATTCCTCTGAAAATAATGAATGTCAA
GATTGTGAGCTTTGGACTATTAATGAAATCATTAATGGAAAAGAAAGTTTCCCTGGACTA
ATTAGATTAATAAATCAATATTTAGCTTCAATGGAGGTCGACACTGACACACACTGCTCA
ATTCAGCAATATTTGAAATTAATACAGCGCAGAGCTGCCGGAAAATTGTTAACAACTGCC
TCTTGGATACGTAAAGAAGTATTAAATCATCCAGATTATAAGAAGGATTCTATTGTAAGC
GAAAAAATCAACTATGATTTGTTAAAGAAAGCAAAGAAAATTCAAGAAGGTCTCTTACCG
TGTAATGAGCTTATAGGATGTTTCACAAATTCCAAAACGAAAGAAGAAATTCCCCCCGCC
ATTGCGAAACAACATCAACCAAAAATGCCTAATGGAACATCGTAA

>g10195.t1 Gene=g10195 Length=634
MGLLTEGSPLSWEETKNLAQHVRKHGIKQFINLFARLKDRQGDVLKWGDEVEYMIIRFDD
DKQIAQASLRALEILNVLQEPELRDPDGVKSLWRPEYAAYMVEGTPGKPYGGLLAHFNVV
ESSMKYRREEVSKLLKDGEEVMSITSFPRLGCPQFSYPSYDVQPDNPQSAAQSLFFPDEA
IYSGHPRFKNLTRNIRLRRGEKVCINVPVFKDENTKYPIPYSLPETPDHVYMDAMGFGMG
NCCLQLTFQACNINEARVLYDQLTPLCPIMLALTAASPLYRGFLTDIDCRWNVISASVDC
RTKEERGLEPLKENKFRINKSRYDSIDSYLSEEGEKYNDVPLLYNEEDYKTLREGGIDHL
LAQHIAHLFIRDTVSLFSEKIHQNDEEETDHFENIQSTNWQTMRFKPPPPSSSIGWRVEF
RPCELQLTDFENAAIVVFIVLLTRVILSFKLNFLIPISKVDENMQKAQKRNACKEEKFWF
RRNFNGKKEDNNSSENNECQDCELWTINEIINGKESFPGLIRLINQYLASMEVDTDTHCS
IQQYLKLIQRRAAGKLLTTASWIRKEVLNHPDYKKDSIVSEKINYDLLKKAKKIQEGLLP
CNELIGCFTNSKTKEEIPPAIAKQHQPKMPNGTS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g10195.t1 Coils Coil Coil 457 477 -
5 g10195.t1 Gene3D G3DSA:3.30.590.50 - 42 322 1.3E-108
6 g10195.t1 Gene3D G3DSA:3.30.590.50 - 381 470 1.1E-32
4 g10195.t1 Gene3D G3DSA:1.10.8.960 - 557 613 4.5E-18
2 g10195.t1 PANTHER PTHR11164 GLUTAMATE CYSTEINE LIGASE 1 612 1.2E-279
1 g10195.t1 Pfam PF03074 Glutamate-cysteine ligase 230 594 1.2E-179
9 g10195.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 432 -
10 g10195.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 433 455 -
8 g10195.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 456 634 -
3 g10195.t1 SUPERFAMILY SSF55931 Glutamine synthetase/guanido kinase 39 583 1.96E-58

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004357 glutamate-cysteine ligase activity MF
GO:0003824 catalytic activity MF
GO:0006750 glutathione biosynthetic process BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values