General information for g10195.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamate–cysteine ligase.
Parent gene
- The parent gene g10195 is located on chr_1: 8167819..8169830 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10195 | g10195.t10 | TTS | g10195.t10 | 8166281 | 8166281 |
| chr_1 | g10195 | g10195.t10 | isoform | g10195.t10 | 8167233 | 8168817 |
| chr_1 | g10195 | g10195.t10 | exon | g10195.t10.exon1 | 8167233 | 8167752 |
| chr_1 | g10195 | g10195.t10 | cds | g10195.t10.CDS1 | 8167233 | 8167752 |
| chr_1 | g10195 | g10195.t10 | exon | g10195.t10.exon2 | 8167819 | 8168250 |
| chr_1 | g10195 | g10195.t10 | cds | g10195.t10.CDS2 | 8167819 | 8168250 |
| chr_1 | g10195 | g10195.t10 | exon | g10195.t10.exon3 | 8168308 | 8168817 |
| chr_1 | g10195 | g10195.t10 | cds | g10195.t10.CDS3 | 8168308 | 8168585 |
| chr_1 | g10195 | g10195.t10 | TSS | g10195.t10 | 8169174 | 8169174 |
Sequences
>g10195.t10 Gene=g10195 Length=1462
ATGGGATTATTGACAGAAGGCAGTCCACTTTCATGGGAGGAGACAAAAAATTTGGCACAG
CATGTGAGAAAACATGGTATCAAGCAGTTTATTAACTTATTTGCAAGATTAAAAGACCGT
CAAGGTGATGGTAAGTTATGAATGCTGATGAAATTGTAATTATGAAATAACAGTATTCTT
TTTGTCATTGTCGGTTTTTTTAGTACTAAAATGGGGTGATGAAGTTGAGTATATGATTAT
AAGGTTTGATGACGATAAACAAATAGCACAAGCCTCTTTAAGAGCACTTGAAATACTGAA
CGTGTTACAAGAACCGGAACTGCGCGATCCAGATGGAGTAAAGTCACTTTGGCGCCCTGA
ATATGCGGCATATATGGTCGAAGGTACACCTGGTAAACCCTATGGAGGGCTATTAGCTCA
CTTTAATGTTGTTGAATCATCAATGAAATACCGTAGAGAAGAAGTGTCCAAGTTGTTAAA
AGATGGTGAAGAAGTTATGTCGATTACAAGTTTTCCACGTTTGGGTTGTCCACAATTTTC
ATATCCATCATATGATGTGCAACCAGACAATCCACAATCTGCAGCTCAATCATTATTCTT
TCCTGATGAAGCTATATATTCGGGTCATCCACGTTTCAAAAATTTAACGCGAAATATTAG
ATTAAGACGAGGTGAAAAGGTGTGCATCAACGTTCCAGTTTTTAAAGATGAAAACACAAA
GTATCCTATTCCTTATTCACTGCCTGAAACGCCTGATCATGTTTATATGGATGCGATGGG
TTTCGGTATGGGAAATTGTTGCTTGCAATTAACATTTCAAGCGTGTAATATCAATGAAGC
TAGAGTTCTTTATGATCAATTGACTCCTCTCTGTCCAATTATGTTAGCATTGACTGCTGC
ATCTCCCTTATATAGGGGCTTTTTAACTGATATCGATTGCAGATGGAATGTTATTTCAGC
ATCTGTAGATTGTAGAACAAAAGAGGAAAGAGGTCTTGAACCTCTAAAAGAAAATAAATT
TAGAATCAACAAGTCACGCTATGATTCAATCGATTCTTATCTATCTGAGGAGGGCGAAAA
ATACAACGACGTTCCTTTACTTTACAATGAAGAAGACTATAAAACTTTACGTGAAGGTGG
AATTGATCATTTATTGGCACAGCATATTGCCCATTTATTTATTCGGGATACTGTTTCTTT
ATTCAGTGAAAAAATTCATCAAAATGATGAAGAAGAGACCGATCATTTCGAAAACATTCA
ATCAACAAATTGGCAAACAATGCGATTTAAACCACCACCACCTTCGAGTAGCATTGGCTG
GCGAGTCGAATTCCGTCCTTGTGAACTCCAATTAACGGATTTTGAGAATGCTGCAATTGT
TGTTTTCATTGTATTGTTAACGCGTGTTATACTCTCATTCAAACTTAATTTCCTTATACC
AATCAGTAAAGTTGATGAGAAT
>g10195.t10 Gene=g10195 Length=410
MIIRFDDDKQIAQASLRALEILNVLQEPELRDPDGVKSLWRPEYAAYMVEGTPGKPYGGL
LAHFNVVESSMKYRREEVSKLLKDGEEVMSITSFPRLGCPQFSYPSYDVQPDNPQSAAQS
LFFPDEAIYSGHPRFKNLTRNIRLRRGEKVCINVPVFKDENTKYPIPYSLPETPDHVYMD
AMGFGMGNCCLQLTFQACNINEARVLYDQLTPLCPIMLALTAASPLYRGFLTDIDCRWNV
ISASVDCRTKEERGLEPLKENKFRINKSRYDSIDSYLSEEGEKYNDVPLLYNEEDYKTLR
EGGIDHLLAQHIAHLFIRDTVSLFSEKIHQNDEEETDHFENIQSTNWQTMRFKPPPPSSS
IGWRVEFRPCELQLTDFENAAIVVFIVLLTRVILSFKLNFLIPISKVDEN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g10195.t10 | Gene3D | G3DSA:3.30.590.50 | - | 1 | 269 | 2.8E-100 |
| 5 | g10195.t10 | Gene3D | G3DSA:3.30.590.50 | - | 328 | 410 | 4.3E-29 |
| 2 | g10195.t10 | PANTHER | PTHR11164 | GLUTAMATE CYSTEINE LIGASE | 1 | 410 | 1.4E-192 |
| 1 | g10195.t10 | Pfam | PF03074 | Glutamate-cysteine ligase | 177 | 410 | 2.6E-125 |
| 7 | g10195.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 379 | - |
| 8 | g10195.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 380 | 402 | - |
| 6 | g10195.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 403 | 410 | - |
| 3 | g10195.t10 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 35 | 410 | 4.71E-43 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004357 | glutamate-cysteine ligase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
