General information for g10195.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamate–cysteine ligase catalytic subunit.
Parent gene
- The parent gene g10195 is located on chr_1: 8167819..8169830 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10195 | g10195.t3 | isoform | g10195.t3 | 8166261 | 8168249 |
| chr_1 | g10195 | g10195.t3 | exon | g10195.t3.exon1 | 8166261 | 8166832 |
| chr_1 | g10195 | g10195.t3 | TTS | g10195.t3 | 8166281 | 8166281 |
| chr_1 | g10195 | g10195.t3 | cds | g10195.t3.CDS1 | 8166649 | 8166832 |
| chr_1 | g10195 | g10195.t3 | exon | g10195.t3.exon2 | 8166901 | 8167752 |
| chr_1 | g10195 | g10195.t3 | cds | g10195.t3.CDS2 | 8166901 | 8167752 |
| chr_1 | g10195 | g10195.t3 | exon | g10195.t3.exon3 | 8167819 | 8168249 |
| chr_1 | g10195 | g10195.t3 | cds | g10195.t3.CDS3 | 8167819 | 8167994 |
| chr_1 | g10195 | g10195.t3 | TSS | g10195.t3 | 8169174 | 8169174 |
Sequences
>g10195.t3 Gene=g10195 Length=1855
TTTCCACGTTTGGGTTGTCCACAATTTTCATATCCATCATATGATGTGCAACCAGACAAT
CCACAATCTGCAGCTCAATCATTATTCTTTCCTGATGAAGCTATATATTCGGGTCATCCA
CGTTTCAAAAATTTAACGCGAAATATTAGATTAAGACGAGGTGAAAAGGTGTGCATCAAC
GTTCCAGTTTTTAAAGATGAAAACACAAAGTATCCTATTCCTTATTCACTGCCTGAAACG
CCTGATCATGTTTATATGGATGCGATGGGTTTCGGTATGGGAAATTGTTGCTTGCAATTA
ACATTTCAAGCGTGTAATATCAATGAAGCTAGAGTTCTTTATGATCAATTGACTCCTCTC
TGTCCAATTATGTTAGCATTGACTGCTGCATCTCCCTTATATAGGGGCTTTTTAACTGAT
ATCGATTGCAGATGGAATGTTATTTCAGCATCTGTAGATTGTAGAACAAAAGAGGAAAGA
GGTCTTGAACCTCTAAAAGAAAATAAATTTAGAATCAACAAGTCACGCTATGATTCAATC
GATTCTTATCTATCTGAGGAGGGCGAAAAATACAACGACGTTCCTTTACTTTACAATGAA
GAAGACTATAAAACTTTACGTGAAGGTGGAATTGATCATTTATTGGCACAGCATATTGCC
CATTTATTTATTCGGGATACTGTTTCTTTATTCAGTGAAAAAATTCATCAAAATGATGAA
GAAGAGACCGATCATTTCGAAAACATTCAATCAACAAATTGGCAAACAATGCGATTTAAA
CCACCACCACCTTCGAGTAGCATTGGCTGGCGAGTCGAATTCCGTCCTTGTGAACTCCAA
TTAACGGATTTTGAGAATGCTGCAATTGTTGTTTTCATTGTATTGTTAACGCGTGTTATA
CTCTCATTCAAACTTAATTTCCTTATACCAATCAGTAAAGTTGATGAGAATATGCAAAAG
GCACAAAAACGAAATGCTTGTAAAGAAGAAAAATTTTGGTTCCGAAGAAATTTCAACGGT
AAAAAAGAAGACAATAATTCCTCTGAAAATAATGAATGTCAAGATTGTGAGCTTTGGACT
ATTAATGAAATCATTAATGGAAAAGAAAGTTTCCCTGGACTAATTAGATTAATAAATCAA
TATTTAGCTTCAATGGAGGTCGACACTGACACACACTGCTCAATTCAGCAATATTTGAAA
TTAATACAGCGCAGAGCTGCCGGAAAATTGTTAACAACTGCCTCTTGGATACGTAAAGAA
GTATTAAATCATCCAGATTATAAGAAGGATTCTATTGTAAGCGAAAAAATCAACTATGAT
TTGTTAAAGAAAGCAAAGAAAATTCAAGAAGGTCTCTTACCGTGTAATGAGCTTATAGGA
TGTTTCACAAATTCCAAAACGAAAGAAGAAATTCCCCCCGCCATTGCGAAACAACATCAA
CCAAAAATGCCTAATGGAACATCGTAATGGATTTGTAATGATAATTGAATGTCGCCTATG
AATACTAGAAAAATAGAAAGTGCACTTAATTATTAATTACATTTGATGATAGTGAACATG
GCGATTAAATTTTTAAGAAAGAGAGAGATAATATAAATGTATGTTAATGTTTCATATAGT
AATGATATATGCATATTATATATTTTAATAGAACTAACTACCTTCAAATATTAACTTTTT
AAATTATGCTTATTGCTTATACAGTATAAATTTTTTTAGTATATAGTAGTACAAAAGACA
CATTCTTACATACTTATATGTTTCAATATACTTTTTCTTATAAAACTGTAACTTGATAAA
ATTTATAATAAAGTAGTTCAAAGTTGGTATCATATAGAAACAGCATTATTTTATG
>g10195.t3 Gene=g10195 Length=403
MDAMGFGMGNCCLQLTFQACNINEARVLYDQLTPLCPIMLALTAASPLYRGFLTDIDCRW
NVISASVDCRTKEERGLEPLKENKFRINKSRYDSIDSYLSEEGEKYNDVPLLYNEEDYKT
LREGGIDHLLAQHIAHLFIRDTVSLFSEKIHQNDEEETDHFENIQSTNWQTMRFKPPPPS
SSIGWRVEFRPCELQLTDFENAAIVVFIVLLTRVILSFKLNFLIPISKVDENMQKAQKRN
ACKEEKFWFRRNFNGKKEDNNSSENNECQDCELWTINEIINGKESFPGLIRLINQYLASM
EVDTDTHCSIQQYLKLIQRRAAGKLLTTASWIRKEVLNHPDYKKDSIVSEKINYDLLKKA
KKIQEGLLPCNELIGCFTNSKTKEEIPPAIAKQHQPKMPNGTS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g10195.t3 | Coils | Coil | Coil | 226 | 246 | - |
| 6 | g10195.t3 | Gene3D | G3DSA:3.30.590.50 | - | 1 | 91 | 9.0E-42 |
| 5 | g10195.t3 | Gene3D | G3DSA:3.30.590.50 | - | 150 | 239 | 5.7E-33 |
| 4 | g10195.t3 | Gene3D | G3DSA:1.10.8.960 | - | 326 | 383 | 2.3E-18 |
| 2 | g10195.t3 | PANTHER | PTHR11164 | GLUTAMATE CYSTEINE LIGASE | 1 | 382 | 3.0E-189 |
| 1 | g10195.t3 | Pfam | PF03074 | Glutamate-cysteine ligase | 1 | 363 | 1.6E-178 |
| 9 | g10195.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 201 | - |
| 10 | g10195.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 202 | 224 | - |
| 8 | g10195.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 225 | 403 | - |
| 3 | g10195.t3 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 11 | 352 | 2.26E-42 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004357 | glutamate-cysteine ligase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K11204 (glutamate–cysteine ligase catalytic subunit)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
