General information for g10195.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamate–cysteine ligase.
Parent gene
- The parent gene g10195 is located on chr_1: 8167819..8169830 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10195 | g10195.t4 | isoform | g10195.t4 | 8166261 | 8169174 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon1 | 8166261 | 8166832 |
| chr_1 | g10195 | g10195.t4 | TTS | g10195.t4 | 8166281 | 8166281 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon2 | 8166901 | 8167488 |
| chr_1 | g10195 | g10195.t4 | cds | g10195.t4.CDS1 | 8167340 | 8167488 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon3 | 8167559 | 8167752 |
| chr_1 | g10195 | g10195.t4 | cds | g10195.t4.CDS2 | 8167559 | 8167752 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon4 | 8167819 | 8168250 |
| chr_1 | g10195 | g10195.t4 | cds | g10195.t4.CDS3 | 8167819 | 8168250 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon5 | 8168308 | 8168614 |
| chr_1 | g10195 | g10195.t4 | cds | g10195.t4.CDS4 | 8168308 | 8168614 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon6 | 8168688 | 8168846 |
| chr_1 | g10195 | g10195.t4 | cds | g10195.t4.CDS5 | 8168688 | 8168817 |
| chr_1 | g10195 | g10195.t4 | exon | g10195.t4.exon7 | 8169064 | 8169174 |
| chr_1 | g10195 | g10195.t4 | TSS | g10195.t4 | 8169174 | 8169174 |
Sequences
>g10195.t4 Gene=g10195 Length=2363
AGTACTCATTGTGCGCTTGCCGCAGAAAGACTAAAATTATTTAAACAAATCTAATATCAG
CCAAGTGTTTTTTTTTATAAATAATTTTTGACAACCACTTTTCGTTTACAGAATATCACT
ACGTTTTAAATTAATTTGTAATGGGATTATTGACAGAAGGCAGTCCACTTTCATGGGAGG
AGACAAAAAATTTGGCACAGCATGTGAGAAAACATGGTATCAAGCAGTTTATTAACTTAT
TTGCAAGATTAAAAGACCGTCAAGGTGATGTACTAAAATGGGGTGATGAAGTTGAGTATA
TGATTATAAGGTTTGATGACGATAAACAAATAGCACAAGCCTCTTTAAGAGCACTTGAAA
TACTGAACGTGTTACAAGAACCGGAACTGCGCGATCCAGATGGAGTAAAGTCACTTTGGC
GCCCTGAATATGCGGCATATATGGTCGAAGGTACACCTGGTAAACCCTATGGAGGGCTAT
TAGCTCACTTTAATGTTGTTGAATCATCAATGAAATACCGTAGAGAAGAAGTGTCCAAGT
TGTTAAAAGATGGTGAAGAAGTTATGTCGATTACAAGTTTTCCACGTTTGGGTTGTCCAC
AATTTTCATATCCATCATATGATGTGCAACCAGACAATCCACAATCTGCAGCTCAATCAT
TATTCTTTCCTGATGAAGCTATATATTCGGGTCATCCACGTTTCAAAAATTTAACGCGAA
ATATTAGATTAAGACGAGGTGAAAAGGTGTGCATCAACGTTCCAGTTTTTAAAGATGAAA
ACACAAAGTATCCTATTCCTTATTCACTGCCTGAAACGCCTGATCATGTTTATATGGATG
CGATGGGTTTCGGTATGGGAAATTGTTGCTTGCAATTAACATTTCAAGCGTGTAATATCA
ATGAAGCTAGAGTTCTTTATGATCAATTGACTCCTCTCTGTCCAATTATGTTAGCATTGA
CTGCTGCATCTCCCTTATATAGGGGCTTTTTAACTGATATCGATTGCAGATGGAATGTTA
TTTCAGCATCTGTAGATTGTAGAACAAAAGAGGAAAGAGGTCTTGAACCTCTAAAAGAAA
ATAAATTTAGAATCAACAAGTCACGCTATGATTCAATCGATTCTTATCTATCTGAGGAGG
GCGAAAAATACAACGACGTTCCTTTACTTTACAATGAAGAAGACTATAAAACTTTACGTG
AAGTGAAAAAATTCATCAAAATGATGAAGAAGAGACCGATCATTTCGAAAACATTCAATC
AACAAATTGGCAAACAATGCGATTTAAACCACCACCACCTTCGAGTAGCATTGGCTGGCG
AGTCGAATTCCGTCCTTGTGAACTCCAATTAACGGATTTTGAGAATGCTGCAATTGTTGT
TTTCATTGTATTGTTAACGCGTGTTATACTCTCATTCAAACTTAATTTCCTTATACCAAT
CAGTAAAGTTGATGAGAATATGCAAAAGGCACAAAAACGAAATGCTTGTAAAGAAGAAAA
ATTTTGGTTCCGAAGAAATTTCAACGGTAAAAAAGAAGACAATAATTCCTCTGAAAATAA
TGAATGTCAAGATTGTGAGCTTTGGACTATTAATGAAATCATTAATGGAAAAGAAAGTTT
CCCTGGACTAATTAGATTAATAAATCAATATTTAGCTTCAATGGAGGTCGACACTGACAC
ACACTGCTCAATTCAGCAATATTTGAAATTAATACAGCGCAGAGCTGCCGGAAAATTGTT
AACAACTGCCTCTTGGATACGTAAAGAAGTATTAAATCATCCAGATTATAAGAAGGATTC
TATTGTAAGCGAAAAAATCAACTATGATTTGTTAAAGAAAGCAAAGAAAATTCAAGAAGG
TCTCTTACCGTGTAATGAGCTTATAGGATGTTTCACAAATTCCAAAACGAAAGAAGAAAT
TCCCCCCGCCATTGCGAAACAACATCAACCAAAAATGCCTAATGGAACATCGTAATGGAT
TTGTAATGATAATTGAATGTCGCCTATGAATACTAGAAAAATAGAAAGTGCACTTAATTA
TTAATTACATTTGATGATAGTGAACATGGCGATTAAATTTTTAAGAAAGAGAGAGATAAT
ATAAATGTATGTTAATGTTTCATATAGTAATGATATATGCATATTATATATTTTAATAGA
ACTAACTACCTTCAAATATTAACTTTTTAAATTATGCTTATTGCTTATACAGTATAAATT
TTTTTAGTATATAGTAGTACAAAAGACACATTCTTACATACTTATATGTTTCAATATACT
TTTTCTTATAAAACTGTAACTTGATAAAATTTATAATAAAGTAGTTCAAAGTTGGTATCA
TATAGAAACAGCATTATTTTATG
>g10195.t4 Gene=g10195 Length=403
MGLLTEGSPLSWEETKNLAQHVRKHGIKQFINLFARLKDRQGDVLKWGDEVEYMIIRFDD
DKQIAQASLRALEILNVLQEPELRDPDGVKSLWRPEYAAYMVEGTPGKPYGGLLAHFNVV
ESSMKYRREEVSKLLKDGEEVMSITSFPRLGCPQFSYPSYDVQPDNPQSAAQSLFFPDEA
IYSGHPRFKNLTRNIRLRRGEKVCINVPVFKDENTKYPIPYSLPETPDHVYMDAMGFGMG
NCCLQLTFQACNINEARVLYDQLTPLCPIMLALTAASPLYRGFLTDIDCRWNVISASVDC
RTKEERGLEPLKENKFRINKSRYDSIDSYLSEEGEKYNDVPLLYNEEDYKTLREVKKFIK
MMKKRPIISKTFNQQIGKQCDLNHHHLRVALAGESNSVLVNSN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g10195.t4 | Gene3D | G3DSA:3.30.590.50 | - | 42 | 322 | 0 |
| 2 | g10195.t4 | PANTHER | PTHR11164 | GLUTAMATE CYSTEINE LIGASE | 1 | 354 | 0 |
| 1 | g10195.t4 | Pfam | PF03074 | Glutamate-cysteine ligase | 230 | 355 | 0 |
| 3 | g10195.t4 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 39 | 347 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004357 | glutamate-cysteine ligase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
