General information for g10199.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Soluble guanylate cyclase 88E.
Parent gene
- The parent gene g10199 is located on chr_1: 8183642..8191400 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10199 | g10199.t1 | isoform | g10199.t1 | 8183642 | 8191400 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon1 | 8183642 | 8183809 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS1 | 8183642 | 8183809 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon2 | 8184866 | 8185041 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS2 | 8184866 | 8185041 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon3 | 8185320 | 8185395 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS3 | 8185320 | 8185395 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon4 | 8185475 | 8185504 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS4 | 8185475 | 8185504 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon5 | 8186255 | 8186460 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS5 | 8186255 | 8186460 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon6 | 8186631 | 8186754 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS6 | 8186631 | 8186754 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon7 | 8186822 | 8187015 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS7 | 8186822 | 8187015 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon8 | 8187312 | 8187383 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS8 | 8187312 | 8187383 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon9 | 8188742 | 8188946 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS9 | 8188742 | 8188946 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon10 | 8189033 | 8189206 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS10 | 8189033 | 8189206 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon11 | 8189853 | 8190034 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS11 | 8189853 | 8190034 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon12 | 8190123 | 8190765 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS12 | 8190123 | 8190765 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon13 | 8190829 | 8191139 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS13 | 8190829 | 8191139 |
| chr_1 | g10199 | g10199.t1 | exon | g10199.t1.exon14 | 8191214 | 8191400 |
| chr_1 | g10199 | g10199.t1 | cds | g10199.t1.CDS14 | 8191214 | 8191400 |
| chr_1 | g10199 | g10199.t1 | TSS | g10199.t1 | NA | NA |
| chr_1 | g10199 | g10199.t1 | TTS | g10199.t1 | NA | NA |
Sequences
>g10199.t1 Gene=g10199 Length=2748
ATGTATGGTTTACTGCTAGAAAATTTAGCAGAATATGTTAAAGCATACTATGGTGACGAA
AAGTGGGATGAAGTACGACGTCAGGCTGGCATATCATCAACTTCATTCAGTGTTCACGAA
GACTACGATGAAGATCTGCTCAATCGGCTAGCAGCAACCGCACAAAATCTTCTTGGTGTA
TCAGAGTCAGAGTTCATGGACCAAATGGGTGTTTACTTTGTAAACTTTGTCGGCCAGTAT
GGATATGATCGTGTTCTATCAGTTCTCGGACGACATATGAGAGATTTCTTAAATGGCTTA
GACAATCTTCATGAATATCTCAAATTTAGCTATCCAATGATGAGAGCGCCATCATTTTTT
TGTGAGAATGAAACGCGACATGGCTTGACGCTACATTATCGCACAAGGAGAAAAGGCTTT
GTTTACTATACAATGGGACAAATTAGACAAGTTGCAAAACATTTTTACAATAAGGATATG
CAAATCGAGCTGGTGAAATCGGATTTTTTGGGCGAAACAAATCACTACACATTTCAATTA
ACATTCGATAATCGAGCTTTTTCATTGGCCTCTCTGGCGATGACTCGCGAAGAAAAGCAT
CTGCCTATAAGTGCATCAGTATTATTCGACATTTTTCCTTTCTGTATTATTTTTGGTTCC
GACATGGTGGTGCGAAGTTTAGGAAATTCATTAATGGTCATTTTACCAGATTTAATAAAT
AAACGAATAATAGACTGGTTTGATTTGAAACGGCCATTAATTGCCTTCAAATTTCAAACA
ATATTAAATAGGACCAATAATATATTTGAGCTAGTCACAGTTAAACCAGTAAAAGCAGTT
GATCAGCGACAAACAGAGCAATTAATATTAAAGGAAAATGACAACGATGATGATAGTGAA
CGAAAGTTGAGGCTAAAAGGTCAAATGATCTATATGACTGACTGGCGGCTGATAATGTTT
TTAGGAACACCTGTGATGCCAAAATTGTCCTCACTGCTATCGACCGGTCTCTATATCAAC
GATTTATCAATGCATGATTTCAGCAGGGATATTCTTTTAGCTGGAACACAGCAGTCGGTA
GAATTGAAATTGGCATTAGATCAAGAACAACAAAAATCGAAAAAGTTGGAAGAATCAATG
CGAAAATTGGACGAGGAAATGAGACGAACGGATGAGTTGTTATATCAGATGATACCAAAG
CAAGTAGCTGATAGACTGCGTAGAGGCGAGAACCCGATTGATACGTGTGAGATGTTTGAT
AGCGTCTCGATATTGTTCTCGGATGTTGTCACTTTTACAGAAATTTGCAGCAGAATAACT
CCAATGGAAGTTGTTTCAATGCTTAACGCAATGTACTCTATATTCGATACATTAACCGAA
CGCAATAATGTGTATAAAGTGGAGACAATTGGGGATGCTTACATGGTTGTGTCAGGCGCT
CCGGAAAAAGAGAAAAATCATGCCGAGAAGGTTTGCGATATGGCGCTTGATATGATAGAA
GCAATAACTGATCTAAAAGATCCTTCAACTGGTCATCATCTCAGGATTCGAGTAGGCGTT
CATTCGGGTGCTGTTGTTGCAGGCATTGTCGGTTTAAAAATGCCACGTTACTGCTTATTT
GGTGATTCAGTGAATACTGCTAGTCGTATGGAATCAACAAGTGAAGCAATGAAAATTCAC
ATATCACAGACAACGAGAAATGCGTTGCCAAAAAATTATAAGGTCAGCGAACGTGGAGAA
ATTGAAGTAAAAGGCAAGGGCACAATGAAAACTTATTGGTTAGATTATCGTGAAAATCGT
AAAACTCTCGCACAATCTAATTCTAATCCCAATCATAGTCCGGTAAAAGCAATCGAATAT
AACTATGATCGTCGTATGAGTATGCCGCAATACATGCTCAATAATAAATCAGAAAATAAT
GTCGATGAGAGGCGTGTATATTCGCCAATCACATTTGAAGATGTTGCAAGACGCTCAATA
ATCAATTCGCCTATAAGGAATTTGTTTAGTGCTCATGGACGCGTTTCTCGTTCAAATTCA
ACTGGCCATGCTTTTTTGCAAAGTCCAAGTGATGTTTTTGGTTCATTAATTACTGATACT
GAAGAATTTTTGGAAGATCTTCATAATCGAAACTCGGCTGGTACGAGTAGTATTTATTCA
CCATTATCAACTCCACTTTCAGTCTATAGTCAATCTTCAGCCAATTATCGAACTTCAACT
GCTAGAACTAAAAAGCATACTGCTGGACAGTTTACGGATGAAGAGCTTATACAACTAAAC
CAAAGAAAACCTTCACCATCGGCACCTGCTTTACCGAAAAACGATGGCACAGCACAAAGC
GTTAATAAGATCGAGAAGGGAATATTGAAGACGAAGGGCAAGAGCAATAAATTTAATCAA
GTGGATTCACAGAAAAAAGCTTTAGAGAATTTAGATCAAATGGTTAAAAATATTTACGAC
ACTGATATACCAGGCATGTGTGCTTTAATGACATTGCCTCCTCCGCCCCCAAATTCAAAA
TCTGAAGGAAATATTGGATATCTTCAGGGTGCTGGTTGCAATAACTATGATGGATATTGC
ACAAATAGTTATCGACAACATCGTTGTATGCCGAATAGCCATAGCTTTACTTATAACAAG
GACCATTGCTGCAGTGGATGCAGTCATCATCATCATCATTCGAGTCACACGAACGGTCGT
CATCATCATCGATTTCATCATCCAAACGGACATTGTTGCATTTTGTAG
>g10199.t1 Gene=g10199 Length=915
MYGLLLENLAEYVKAYYGDEKWDEVRRQAGISSTSFSVHEDYDEDLLNRLAATAQNLLGV
SESEFMDQMGVYFVNFVGQYGYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPMMRAPSFF
CENETRHGLTLHYRTRRKGFVYYTMGQIRQVAKHFYNKDMQIELVKSDFLGETNHYTFQL
TFDNRAFSLASLAMTREEKHLPISASVLFDIFPFCIIFGSDMVVRSLGNSLMVILPDLIN
KRIIDWFDLKRPLIAFKFQTILNRTNNIFELVTVKPVKAVDQRQTEQLILKENDNDDDSE
RKLRLKGQMIYMTDWRLIMFLGTPVMPKLSSLLSTGLYINDLSMHDFSRDILLAGTQQSV
ELKLALDQEQQKSKKLEESMRKLDEEMRRTDELLYQMIPKQVADRLRRGENPIDTCEMFD
SVSILFSDVVTFTEICSRITPMEVVSMLNAMYSIFDTLTERNNVYKVETIGDAYMVVSGA
PEKEKNHAEKVCDMALDMIEAITDLKDPSTGHHLRIRVGVHSGAVVAGIVGLKMPRYCLF
GDSVNTASRMESTSEAMKIHISQTTRNALPKNYKVSERGEIEVKGKGTMKTYWLDYRENR
KTLAQSNSNPNHSPVKAIEYNYDRRMSMPQYMLNNKSENNVDERRVYSPITFEDVARRSI
INSPIRNLFSAHGRVSRSNSTGHAFLQSPSDVFGSLITDTEEFLEDLHNRNSAGTSSIYS
PLSTPLSVYSQSSANYRTSTARTKKHTAGQFTDEELIQLNQRKPSPSAPALPKNDGTAQS
VNKIEKGILKTKGKSNKFNQVDSQKKALENLDQMVKNIYDTDIPGMCALMTLPPPPPNSK
SEGNIGYLQGAGCNNYDGYCTNSYRQHRCMPNSHSFTYNKDHCCSGCSHHHHHSSHTNGR
HHHRFHHPNGHCCIL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g10199.t1 | CDD | cd07302 | CHD | 422 | 594 | 7.97929E-70 |
| 11 | g10199.t1 | Coils | Coil | Coil | 359 | 393 | - |
| 10 | g10199.t1 | Gene3D | G3DSA:3.90.1520.10 | - | 1 | 189 | 7.4E-61 |
| 9 | g10199.t1 | Gene3D | G3DSA:3.30.450.260 | - | 196 | 340 | 4.2E-26 |
| 8 | g10199.t1 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 414 | 597 | 1.3E-77 |
| 4 | g10199.t1 | PANTHER | PTHR45655 | GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2 | 2 | 610 | 0.0 |
| 5 | g10199.t1 | PANTHER | PTHR45655:SF10 | SOLUBLE GUANYLATE CYCLASE 88E | 2 | 610 | 0.0 |
| 1 | g10199.t1 | Pfam | PF07700 | Haem-NO-binding | 2 | 163 | 1.6E-53 |
| 2 | g10199.t1 | Pfam | PF07701 | Heme NO binding associated | 202 | 406 | 1.7E-67 |
| 3 | g10199.t1 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 417 | 594 | 9.2E-68 |
| 13 | g10199.t1 | ProSitePatterns | PS00452 | Guanylate cyclase signature. | 528 | 551 | - |
| 15 | g10199.t1 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 423 | 551 | 48.79 |
| 14 | g10199.t1 | SMART | SM00044 | cyc_6 | 385 | 579 | 1.1E-91 |
| 6 | g10199.t1 | SUPERFAMILY | SSF111126 | Ligand-binding domain in the NO signalling and Golgi transport | 1 | 188 | 4.13E-50 |
| 7 | g10199.t1 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 412 | 594 | 2.35E-63 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006182 | cGMP biosynthetic process | BP |
| GO:0020037 | heme binding | MF |
| GO:0016849 | phosphorus-oxygen lyase activity | MF |
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0035556 | intracellular signal transduction | BP |
| GO:0004383 | guanylate cyclase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01769 (guanylate cyclase, other)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
