General information for g10214.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Translocating chain-associated membrane protein 1-like 1.
Parent gene
- The parent gene g10214 is located on chr_1: 8264765..8265878 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10214 | g10214.t10 | TSS | g10214.t10 | 8263065 | 8263065 |
| chr_1 | g10214 | g10214.t10 | isoform | g10214.t10 | 8264082 | 8265436 |
| chr_1 | g10214 | g10214.t10 | exon | g10214.t10.exon1 | 8264082 | 8264200 |
| chr_1 | g10214 | g10214.t10 | cds | g10214.t10.CDS1 | 8264168 | 8264200 |
| chr_1 | g10214 | g10214.t10 | exon | g10214.t10.exon2 | 8264259 | 8264700 |
| chr_1 | g10214 | g10214.t10 | cds | g10214.t10.CDS2 | 8264259 | 8264700 |
| chr_1 | g10214 | g10214.t10 | exon | g10214.t10.exon3 | 8264757 | 8265436 |
| chr_1 | g10214 | g10214.t10 | cds | g10214.t10.CDS3 | 8264757 | 8265106 |
| chr_1 | g10214 | g10214.t10 | TTS | g10214.t10 | 8265855 | 8265855 |
Sequences
>g10214.t10 Gene=g10214 Length=1241
TGCTATCTAGAAACGAGCCTTTCTTATATGAATCTGGTTGGAGAGATGGACCTGCTGTTT
TCTTTTATACTCTTATCTGTATAATCATGCATGCAATTTTGCAAGAATATGTTTTAGATA
AAGTTTCCAAGAAACTTCATCTCTCAAAGTTCAAACTTTCACGATTCAATGAGTCTGGTC
AACTTGTTGTCTTTTACTTAATGTCGTTCCTTTGGGGTGCTGAAATCATCATTCGTGAGC
AATATGCTGAAAATATGTCAAAACTTTGGGTAGATTTCCCAAATCATCCAATGACATTCT
TGCACAAGCTCTACTTCATCATTCAGCTTGCTTACTACTTACATATGTTGCCAGAACTTT
ATTTCCAAAAGATTAAGAAAGATGAACAGCTACCAAAGATTCAACATTCTATTGCAGGGA
CACTTATTATTGGCTTCTTCTATTTCTTCGCTTATCGTCGTATTGCCATTGTTTTGTTGA
CTCTTCATTATTTCAGCGAATTTGTTGCACATACATTTGGACTTCTTGAAGTCTTTGACA
AGGAAGAGAAATATGCTAAATTCCGCGTTTTCAACAACATCATTTTCCTCCTTACCGCCT
TCTCGACCTTGGTTCTTTCATTCCTCACTTTCTTCACTGGAATAGGAAAATCAAATCCAA
CAAGTGGCATGACTGGTTTACTCATTGCCTTTTCTATTCAAGGCTTTCTCATTTTCCGAT
TTATTACAAGTTTCCTCAAATCAAAACGTGAATCAAAGGAGCAGCAAGCCCTTAAACAGC
CATTGACAAAGAAATCAAAGGCTAGTGGTGAAACAAAGAATGCTGCTTCAAGTAAAAAAG
ACCGCAAGAAGGAATCAGATTTGCCAGAAGCAGATCAAAATGTAAGCCAAAAGAAAGTCA
AGACCAAGTAAATTAAAATTGATTTCCTTTTTTGTTAACACCTGATGATTCTGATTATTC
TCTTTTATACTTCCTTTCTATACTTGCTTCAGTTGATTTTTTTTAAAAATTCTATCTAAG
TACATTCATTAAAAAAAAGTTCTAATATTTTTGTAACAGTTCATTCTTCATGTAAGAGCA
TTTATTGTAATTTTCTATATATAAAAAGAAGAAAATCTATATTTTTACATTGATCAAAAA
CGAATTTTTTTGGTTGAAATTTTTCTCGTTGCACATTAAATTTTTTTGTAGAAAAAGTTT
TATTAATAAAAAGTAACTAAATGTCCATAGACGTTAGAAAT
>g10214.t10 Gene=g10214 Length=274
MHAILQEYVLDKVSKKLHLSKFKLSRFNESGQLVVFYLMSFLWGAEIIIREQYAENMSKL
WVDFPNHPMTFLHKLYFIIQLAYYLHMLPELYFQKIKKDEQLPKIQHSIAGTLIIGFFYF
FAYRRIAIVLLTLHYFSEFVAHTFGLLEVFDKEEKYAKFRVFNNIIFLLTAFSTLVLSFL
TFFTGIGKSNPTSGMTGLLIAFSIQGFLIFRFITSFLKSKRESKEQQALKQPLTKKSKAS
GETKNAASSKKDRKKESDLPEADQNVSQKKVKTK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g10214.t10 | MobiDBLite | mobidb-lite | consensus disorder prediction | 225 | 274 | - |
| 12 | g10214.t10 | MobiDBLite | mobidb-lite | consensus disorder prediction | 245 | 274 | - |
| 2 | g10214.t10 | PANTHER | PTHR12371:SF11 | TRANSLOCATING CHAIN-ASSOCIATED MEMBRANE PROTEIN | 1 | 270 | 2.9E-80 |
| 3 | g10214.t10 | PANTHER | PTHR12371 | TRANSLOCATION ASSOCIATED MEMBRANE PROTEIN | 1 | 270 | 2.9E-80 |
| 1 | g10214.t10 | Pfam | PF03798 | TLC domain | 26 | 172 | 1.6E-15 |
| 16 | g10214.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 32 | - |
| 21 | g10214.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 33 | 53 | - |
| 18 | g10214.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 54 | 72 | - |
| 24 | g10214.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 73 | 93 | - |
| 15 | g10214.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 94 | 104 | - |
| 26 | g10214.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 105 | 122 | - |
| 20 | g10214.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 123 | 127 | - |
| 23 | g10214.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 128 | 149 | - |
| 17 | g10214.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 150 | 160 | - |
| 25 | g10214.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 161 | 183 | - |
| 19 | g10214.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 184 | 194 | - |
| 22 | g10214.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 195 | 214 | - |
| 14 | g10214.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 215 | 274 | - |
| 13 | g10214.t10 | ProSiteProfiles | PS50922 | TLC domain profile. | 25 | 224 | 9.761 |
| 10 | g10214.t10 | SMART | SM00724 | lag1_27 | 25 | 221 | 2.1E-22 |
| 7 | g10214.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 33 | 50 | - |
| 6 | g10214.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 70 | 92 | - |
| 9 | g10214.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 105 | 122 | - |
| 5 | g10214.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 126 | 148 | - |
| 8 | g10214.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 161 | 183 | - |
| 4 | g10214.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 193 | 215 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006616 | SRP-dependent cotranslational protein targeting to membrane, translocation | BP |
| GO:0016021 | integral component of membrane | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
