General information for g10214.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g10214 is located on chr_1: 8264765..8265878 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10214 | g10214.t8 | TSS | g10214.t8 | 8263065 | 8263065 |
| chr_1 | g10214 | g10214.t8 | isoform | g10214.t8 | 8263386 | 8265878 |
| chr_1 | g10214 | g10214.t8 | exon | g10214.t8.exon1 | 8263386 | 8263583 |
| chr_1 | g10214 | g10214.t8 | exon | g10214.t8.exon2 | 8264199 | 8264700 |
| chr_1 | g10214 | g10214.t8 | cds | g10214.t8.CDS1 | 8264340 | 8264700 |
| chr_1 | g10214 | g10214.t8 | exon | g10214.t8.exon3 | 8264757 | 8265878 |
| chr_1 | g10214 | g10214.t8 | cds | g10214.t8.CDS2 | 8264757 | 8265106 |
| chr_1 | g10214 | g10214.t8 | TTS | g10214.t8 | 8265855 | 8265855 |
Sequences
>g10214.t8 Gene=g10214 Length=1822
ATGTCATCAGCGTTAAGAACGAAACCATTTGGAGGCAAAAAAGATAAGAAGAATCCACCG
ATTCTAAGTCATGAATTTGTTATTCAGAATCATGCAGATATCGTCTCATGTGTTGCAATG
GTGTTTGTCGTCGGTCTAATGATGCAAGTAAGTGTTATCGTATTCATGCAACAGATTTTA
TATCTTTTATACCAGCATATGTGAGTAGAAAGTATTATTGAATCAAAATTTATATTATTT
CTTTTTCAATATTTGTAGAAAGTTTCCAAGAAACTTCATCTCTCAAAGTTCAAACTTTCA
CGATTCAATGAGTCTGGTCAACTTGTTGTCTTTTACTTAATGTCGTTCCTTTGGGGTGCT
GAAATCATCATTCGTGAGCAATATGCTGAAAATATGTCAAAACTTTGGGTAGATTTCCCA
AATCATCCAATGACATTCTTGCACAAGCTCTACTTCATCATTCAGCTTGCTTACTACTTA
CATATGTTGCCAGAACTTTATTTCCAAAAGATTAAGAAAGATGAACAGCTACCAAAGATT
CAACATTCTATTGCAGGGACACTTATTATTGGCTTCTTCTATTTCTTCGCTTATCGTCGT
ATTGCCATTGTTTTGTTGACTCTTCATTATTTCAGCGAATTTGTTGCACATACATTTGGA
CTTCTTGAAGTCTTTGACAAGGAAGAGAAATATGCTAAATTCCGCGTTTTCAACAACATC
ATTTTCCTCCTTACCGCCTTCTCGACCTTGGTTCTTTCATTCCTCACTTTCTTCACTGGA
ATAGGAAAATCAAATCCAACAAGTGGCATGACTGGTTTACTCATTGCCTTTTCTATTCAA
GGCTTTCTCATTTTCCGATTTATTACAAGTTTCCTCAAATCAAAACGTGAATCAAAGGAG
CAGCAAGCCCTTAAACAGCCATTGACAAAGAAATCAAAGGCTAGTGGTGAAACAAAGAAT
GCTGCTTCAAGTAAAAAAGACCGCAAGAAGGAATCAGATTTGCCAGAAGCAGATCAAAAT
GTAAGCCAAAAGAAAGTCAAGACCAAGTAAATTAAAATTGATTTCCTTTTTTGTTAACAC
CTGATGATTCTGATTATTCTCTTTTATACTTCCTTTCTATACTTGCTTCAGTTGATTTTT
TTTAAAAATTCTATCTAAGTACATTCATTAAAAAAAAGTTCTAATATTTTTGTAACAGTT
CATTCTTCATGTAAGAGCATTTATTGTAATTTTCTATATATAAAAAGAAGAAAATCTATA
TTTTTACATTGATCAAAAACGAATTTTTTTGGTTGAAATTTTTCTCGTTGCACATTAAAT
TTTTTTGTAGAAAAAGTTTTATTAATAAAAAGTAACTAAATGTCCATAGACGTTAGAAAT
GACATATTAAAAAAGAGAATATAATTTTAAATGATCCTATTTTTTTTTAGAAACAATGAG
TAAAAGAAATTGTTTTTGATAATGCATAAGCAGCAAGAAAACTTATGACGAAAACATTTT
AACTATTTTTTAAGTTCTATTATTCAGTTAATCATTTATAACATTTAAAAAATCTTAAAA
TCTTAATCATAGACGGAATATTTACTTTTTTACCTCCCACATCCAATTTTCATTCTACAA
TAGGTTTTGTATATTAAATGGTAAAAATTCCGTCTGTGATTTTATAATCTTTTTATTTTT
TTACATTGTAAAAATGAAGAAAATTTCCGATATTTTTTTGAGAGTAAAAAGGCGATGAAA
AAAATATATAAAAGAAGATAAGAAATAAATAATTTTTGTATGTTTCAATGCAAAAAGAGA
ATATTTTGCTGTGTTTTTTAAT
>g10214.t8 Gene=g10214 Length=236
MSFLWGAEIIIREQYAENMSKLWVDFPNHPMTFLHKLYFIIQLAYYLHMLPELYFQKIKK
DEQLPKIQHSIAGTLIIGFFYFFAYRRIAIVLLTLHYFSEFVAHTFGLLEVFDKEEKYAK
FRVFNNIIFLLTAFSTLVLSFLTFFTGIGKSNPTSGMTGLLIAFSIQGFLIFRFITSFLK
SKRESKEQQALKQPLTKKSKASGETKNAASSKKDRKKESDLPEADQNVSQKKVKTK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g10214.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 187 | 236 | - |
| 11 | g10214.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 207 | 236 | - |
| 2 | g10214.t8 | PANTHER | PTHR12371:SF11 | TRANSLOCATING CHAIN-ASSOCIATED MEMBRANE PROTEIN | 1 | 232 | 1.7E-57 |
| 3 | g10214.t8 | PANTHER | PTHR12371 | TRANSLOCATION ASSOCIATED MEMBRANE PROTEIN | 1 | 232 | 1.7E-57 |
| 1 | g10214.t8 | Pfam | PF03798 | TLC domain | 2 | 132 | 4.8E-13 |
| 17 | g10214.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 36 | - |
| 23 | g10214.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 55 | - |
| 14 | g10214.t8 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 56 | 66 | - |
| 21 | g10214.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 67 | 84 | - |
| 18 | g10214.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 85 | 89 | - |
| 22 | g10214.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 90 | 111 | - |
| 13 | g10214.t8 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 112 | 122 | - |
| 19 | g10214.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 123 | 145 | - |
| 16 | g10214.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 146 | 156 | - |
| 20 | g10214.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 157 | 176 | - |
| 15 | g10214.t8 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 177 | 236 | - |
| 12 | g10214.t8 | ProSiteProfiles | PS50922 | TLC domain profile. | 1 | 186 | 8.008 |
| 9 | g10214.t8 | SMART | SM00724 | lag1_27 | 2 | 183 | 4.9E-9 |
| 8 | g10214.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 33 | 55 | - |
| 5 | g10214.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 67 | 84 | - |
| 7 | g10214.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 88 | 110 | - |
| 4 | g10214.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 123 | 145 | - |
| 6 | g10214.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 155 | 177 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006616 | SRP-dependent cotranslational protein targeting to membrane, translocation | BP |
| GO:0016021 | integral component of membrane | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
