General information for g10251.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Adenosine receptor A2a.
Parent gene
- The parent gene g10251 is located on chr_1: 8531734..8533956 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10251 | g10251.t1 | isoform | g10251.t1 | 8531734 | 8533956 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon1 | 8531734 | 8531895 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS1 | 8531734 | 8531895 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon2 | 8531977 | 8532141 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS2 | 8531977 | 8532141 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon3 | 8532197 | 8532313 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS3 | 8532197 | 8532313 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon4 | 8532374 | 8532428 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS4 | 8532374 | 8532428 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon5 | 8532861 | 8533069 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS5 | 8532861 | 8533069 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon6 | 8533139 | 8533324 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS6 | 8533139 | 8533324 |
| chr_1 | g10251 | g10251.t1 | exon | g10251.t1.exon7 | 8533390 | 8533956 |
| chr_1 | g10251 | g10251.t1 | cds | g10251.t1.CDS7 | 8533390 | 8533956 |
| chr_1 | g10251 | g10251.t1 | TTS | g10251.t1 | 8534084 | 8534084 |
| chr_1 | g10251 | g10251.t1 | TSS | g10251.t1 | NA | NA |
Sequences
>g10251.t1 Gene=g10251 Length=1461
ATGTTCAAAACGGAAAATACAACGCTTTGGACAGAAGAACAATTCAATGATTTTGAAATA
TCATCTTCGGATGATTTTTTTTATGCAAATTCTTCAATTTTAAATGCTACAGCAACTCAT
GAAAATGTGGAAAGAAGAACAACACTTAATATTCCATATACTATAATGGAAATACTTGTA
GCAGTAGTTGCAGTAATTGGAAATGGTTTAGTGATTATTGTTTTCTATCGTGAACGAAGA
TTGAGAAGACGAACAAATTATTACATCATATCATTAGCTTTAGCCGATTTATTGGTCGGA
CTCATTGGCATTCCTTTTGCAATTCTTGCTGACATAGGATTACCAAGAAATTTACATGCA
TGTTTATTCACAATTTCTACATTGATAGTTCTCTGTACAATTTCAATATTTTGTTTAGTT
GCAGTTTCTATTGATCGTTATTGGGCGATTTTACACCCTTTAGCATATTCTCGAAATGTA
AGGACAAAGACTGCCATAGGAATCATAAGTCTTTGTTGGCTCTTCGGACTCATTATCGGC
TTTTTGCCACTCTTTGGATGGCACAATGATAGTGGTGTAGAGGAATGTTATTTCACAGAA
AAGATGGATTATAATTATCTCGTATTTCTTTATATAACTACGATAATAACACCTTCAATC
ATACTCGCAGTATTTTATGGACTGATCTATCGTGTCATTCTCAACCAGGTCAATAAAACG
TCTTCTATACGTGTGTCCACAAGAAATAATACTTTAACGACCACAACTTCTTCTTTAAAT
ACACAAAATGAAACTCAGATGTTACGTCTATTAAATTCGGCAGCACAGAAGCGTGAAGTT
AAAGCTACTCAGAATCTCTCAATTATTGTTGTATTTTTCGTCATTTGTTGGATTCCCTTA
TATACAATAAATTGTATAAATGCATTCTGTAGCGAATGCATGGTTAATCCTACAGTTTTG
TATTTTGCAATAATTTTATCACATGTAAACAGTGGCGCAAATCCACTATTTTATGCCTAT
CACTTGAAAGACTTTCGTGGTGCTCTTTCAAGATTATTTGGTTACAGAAATCAACCTGAA
CATTATCGTCCTTCATTAATAAGTCAACAACAACAAAGAATTTCTGAACATTACAGTATG
CGAAGAAATTTTGAACCTCGAATTTATGTTGATTCACCTGTTTGGAAGCGCCAACAGCAA
CAAATACAACTTGAATCAATAAAATCATTTCCAAAGACACATGACAATGATTCTGGAAAT
ATTTCAGCATCAGAGATACCACAAAATGACAAAAATGATAGTGTAAATGGCAGCGAGAAG
CCCACGTCAATTAATACTTATGCACGTCAACCAAAGATTTTTATCTTAAGTGATTCTGTT
ACATGTGAACCGCAAAATAGTTCTTTATATAAACATATTGTAAAACGTAATAGAAATCAC
GAAAATGAAGATTCAATTTAA
>g10251.t1 Gene=g10251 Length=486
MFKTENTTLWTEEQFNDFEISSSDDFFYANSSILNATATHENVERRTTLNIPYTIMEILV
AVVAVIGNGLVIIVFYRERRLRRRTNYYIISLALADLLVGLIGIPFAILADIGLPRNLHA
CLFTISTLIVLCTISIFCLVAVSIDRYWAILHPLAYSRNVRTKTAIGIISLCWLFGLIIG
FLPLFGWHNDSGVEECYFTEKMDYNYLVFLYITTIITPSIILAVFYGLIYRVILNQVNKT
SSIRVSTRNNTLTTTTSSLNTQNETQMLRLLNSAAQKREVKATQNLSIIVVFFVICWIPL
YTINCINAFCSECMVNPTVLYFAIILSHVNSGANPLFYAYHLKDFRGALSRLFGYRNQPE
HYRPSLISQQQQRISEHYSMRRNFEPRIYVDSPVWKRQQQQIQLESIKSFPKTHDNDSGN
ISASEIPQNDKNDSVNGSEKPTSINTYARQPKIFILSDSVTCEPQNSSLYKHIVKRNRNH
ENEDSI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 39 | g10251.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 409 | 444 | - |
| 2 | g10251.t1 | PANTHER | PTHR24246 | OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR | 34 | 352 | 2.1E-75 |
| 3 | g10251.t1 | PANTHER | PTHR24246:SF50 | ADENOSINE RECEPTOR, ISOFORM A | 34 | 352 | 2.1E-75 |
| 11 | g10251.t1 | PRINTS | PR00424 | Adenosine receptor signature | 51 | 60 | 2.5E-8 |
| 8 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 52 | 76 | 3.0E-38 |
| 7 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 85 | 106 | 3.0E-38 |
| 12 | g10251.t1 | PRINTS | PR00424 | Adenosine receptor signature | 120 | 132 | 2.5E-8 |
| 9 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 128 | 150 | 3.0E-38 |
| 10 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 164 | 185 | 3.0E-38 |
| 14 | g10251.t1 | PRINTS | PR00424 | Adenosine receptor signature | 179 | 189 | 2.5E-8 |
| 5 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 207 | 230 | 3.0E-38 |
| 15 | g10251.t1 | PRINTS | PR00424 | Adenosine receptor signature | 273 | 286 | 2.5E-8 |
| 4 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 282 | 306 | 3.0E-38 |
| 6 | g10251.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 320 | 346 | 3.0E-38 |
| 13 | g10251.t1 | PRINTS | PR00424 | Adenosine receptor signature | 320 | 330 | 2.5E-8 |
| 1 | g10251.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 67 | 338 | 1.6E-53 |
| 21 | g10251.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 50 | - |
| 28 | g10251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 51 | 75 | - |
| 18 | g10251.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 76 | 86 | - |
| 27 | g10251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 110 | - |
| 23 | g10251.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 111 | 121 | - |
| 26 | g10251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 144 | - |
| 19 | g10251.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 145 | 164 | - |
| 29 | g10251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 186 | - |
| 20 | g10251.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 187 | 205 | - |
| 25 | g10251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 206 | 229 | - |
| 17 | g10251.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 230 | 285 | - |
| 24 | g10251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 286 | 303 | - |
| 22 | g10251.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 304 | 486 | - |
| 37 | g10251.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 134 | 150 | - |
| 40 | g10251.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 67 | 338 | 41.977 |
| 38 | g10251.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 61 | 353 | 1.1E-5 |
| 16 | g10251.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 28 | 374 | 2.75E-63 |
| 34 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 53 | 75 | - |
| 30 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 109 | - |
| 32 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 144 | - |
| 36 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 187 | - |
| 33 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 207 | 229 | - |
| 31 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 286 | 303 | - |
| 35 | g10251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 318 | 340 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0001609 | G protein-coupled adenosine receptor activity | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0001973 | G protein-coupled adenosine receptor signaling pathway | BP |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04266 (adenosine receptor A2a)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed