General information for g10282.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Membrane-bound transcription factor site-1 protease.
Parent gene
- The parent gene g10282 is located on chr_1: 8625106..8625937 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10282 | g10282.t3 | TSS | g10282.t3 | 8622477 | 8622477 |
| chr_1 | g10282 | g10282.t3 | isoform | g10282.t3 | 8622660 | 8624011 |
| chr_1 | g10282 | g10282.t3 | exon | g10282.t3.exon1 | 8622660 | 8622920 |
| chr_1 | g10282 | g10282.t3 | cds | g10282.t3.CDS1 | 8622660 | 8622920 |
| chr_1 | g10282 | g10282.t3 | exon | g10282.t3.exon2 | 8622983 | 8623502 |
| chr_1 | g10282 | g10282.t3 | cds | g10282.t3.CDS2 | 8622983 | 8623502 |
| chr_1 | g10282 | g10282.t3 | exon | g10282.t3.exon3 | 8623563 | 8624011 |
| chr_1 | g10282 | g10282.t3 | cds | g10282.t3.CDS3 | 8623563 | 8624011 |
| chr_1 | g10282 | g10282.t3 | TTS | g10282.t3 | NA | NA |
Sequences
>g10282.t3 Gene=g10282 Length=1230
ATGATGAAAAAGAGAAAAGATTTCGTAATTTTCTTTATCTTCGTAATAATTAATTTATTC
AATTGTAATACGACATTGGAAAATGAGAAAGATATTCAAGATGAAGGATTAAAATGCTGC
GAGAATGCAACAGATCAACGTTTAATTGTTGAATTTTCATCAAGTATTGTAGAACATGAA
TACATAATACACTTTAATAATTATTATAAGAAAGAAACACGAAGGAAATACATCATTTTA
GCTCTTGATAATAATTCCGAGATCAAAAATTTTACAATTGTTGAAAGACAAAATCCAGCA
AGCGACTACCCAAGTGACTTTGACGTTATTCATCTCTATGAAAAAACTCCACTAAAAGGA
CTTGATTTGCTTATTAAACATCCTCTTATAAAAAGTGTTAGTCCACAACGAATAGTACAT
AGAACGCTAAAATTTCTAAATAAAGATGATGAACAAGAAGAAGAAATAGTAGCAAATAAT
GAAAATGATGGTGATACAAACAAGGTGAATGATAATGAAGATGATATTATCAATTATATT
TCAAAGCATCTTAAACTTGATACGAGAACTGCCAATTTAAAAAATAAGCAAGGAGAATGT
TGTGTGGCTGAATTTCAAAATTTTAGACGAGGTCTATCATCATCAACTTCAATTGATACC
AGTCAACAGCAGCAGCAACAGTTACCGATAGAAAATAATGTGAATTCTCACTCTAATCGA
CGTTTATTAAGAGCAATTCCTAGGCAAATAACATCAATTTTGAAAGCAGACGCATTATGG
AGCATCGGCATCACTGGCAAAGGAGTGAAAGTTGCTGTTTTCGACACAGGTTTAAGTAAA
TCACATCCACATTTTAAGAAAATCAAAGAGAGGACTAATTGGACAAATGAAAAATCATTG
AGTGATGGGATCTCTCATGGAACTTTTGTCACAGGCATTATCGGTTCTTCAAAAGAATGT
TTAGGATTTGCACCAGATGCTGACATTCATATATATCGTGTATTTACAAGTAATCAAGTG
AGCTACACTTCTTGGTTTCTCGATGCATTCAATTATGCAATACTTAAAAAAATCAATGTC
ATTAATTTGAGCATTGGTGGTCCTGATTTTTTGGATCGTCCATTTGTTGATAAAGTACTT
GAGTTATCAGCAAATAAAGTTATAATGGTATCAGCAATAGGCAATGATGGACCATTATAT
GGAACTCTAAATAATCCCGGTGATCAAATG
>g10282.t3 Gene=g10282 Length=410
MMKKRKDFVIFFIFVIINLFNCNTTLENEKDIQDEGLKCCENATDQRLIVEFSSSIVEHE
YIIHFNNYYKKETRRKYIILALDNNSEIKNFTIVERQNPASDYPSDFDVIHLYEKTPLKG
LDLLIKHPLIKSVSPQRIVHRTLKFLNKDDEQEEEIVANNENDGDTNKVNDNEDDIINYI
SKHLKLDTRTANLKNKQGECCVAEFQNFRRGLSSSTSIDTSQQQQQQLPIENNVNSHSNR
RLLRAIPRQITSILKADALWSIGITGKGVKVAVFDTGLSKSHPHFKKIKERTNWTNEKSL
SDGISHGTFVTGIIGSSKECLGFAPDADIHIYRVFTSNQVSYTSWFLDAFNYAILKKINV
INLSIGGPDFLDRPFVDKVLELSANKVIMVSAIGNDGPLYGTLNNPGDQM
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10282.t3 | Gene3D | G3DSA:3.40.50.200 | - | 248 | 409 | 1.4E-38 |
| 2 | g10282.t3 | PANTHER | PTHR43806:SF7 | MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASE | 55 | 410 | 8.0E-110 |
| 3 | g10282.t3 | PANTHER | PTHR43806 | PEPTIDASE S8 | 55 | 410 | 8.0E-110 |
| 5 | g10282.t3 | PRINTS | PR00723 | Subtilisin serine protease family (S8) signature | 266 | 285 | 1.4E-7 |
| 4 | g10282.t3 | PRINTS | PR00723 | Subtilisin serine protease family (S8) signature | 302 | 315 | 1.4E-7 |
| 1 | g10282.t3 | Pfam | PF00082 | Subtilase family | 266 | 407 | 4.3E-21 |
| 10 | g10282.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 11 | g10282.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 7 | - |
| 12 | g10282.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 8 | 17 | - |
| 13 | g10282.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 22 | - |
| 9 | g10282.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 410 | - |
| 14 | g10282.t3 | ProSitePatterns | PS00137 | Serine proteases, subtilase family, histidine active site. | 306 | 316 | - |
| 15 | g10282.t3 | ProSiteProfiles | PS51892 | Serine proteases, subtilase domain profile. | 247 | 410 | 21.641 |
| 6 | g10282.t3 | SUPERFAMILY | SSF52743 | Subtilisin-like | 48 | 408 | 3.27E-39 |
| 7 | g10282.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008236 | serine-type peptidase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed