General information for g10286.t27
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Uridine-cytidine kinase.
Parent gene
- The parent gene g10286 is located on chr_1: 8652184..8652916 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10286 | g10286.t27 | isoform | g10286.t27 | 8648440 | 8652352 |
| chr_1 | g10286 | g10286.t27 | exon | g10286.t27.exon1 | 8648440 | 8648998 |
| chr_1 | g10286 | g10286.t27 | TTS | g10286.t27 | 8648460 | 8648460 |
| chr_1 | g10286 | g10286.t27 | exon | g10286.t27.exon2 | 8650218 | 8650248 |
| chr_1 | g10286 | g10286.t27 | exon | g10286.t27.exon3 | 8651489 | 8651618 |
| chr_1 | g10286 | g10286.t27 | exon | g10286.t27.exon4 | 8651745 | 8652352 |
| chr_1 | g10286 | g10286.t27 | cds | g10286.t27.CDS1 | 8651753 | 8652238 |
| chr_1 | g10286 | g10286.t27 | TSS | g10286.t27 | 8652415 | 8652415 |
Sequences
>g10286.t27 Gene=g10286 Length=1328
ATGGAAATTGAACCTAAAAAACCTTTCCTAATAGGTAAGTATTATTATATTAACTGATTG
ATGTAAAATTTGTAAGAATTGATTTTGATTTCAAATGAAAATTCTAAATTTTTTATGAAT
GAAAATTTTTTTGTTGTGCCACATTTTTTCTTATTCAAAATCTTTATTTGCCATCTCTCA
TTTTTCTCAGGAGTTGCAGGAGGTACCGCAAGTGGCAAAAGTTCAGTATGTAAGCGAATT
ATTGAAAAATTGGGACAAGCTGACATAAATGAACGACAACGTCAAGTAGTAAGCATTAGC
CAGGATTCCTTTTATCGTGAACTTACAGAAGAAGAGAAAATTAAGGCGCAAAAGGGGCAG
TTTAATTTTGATCATCCTCAATCTTTTGACGAAGAGTTAATGTACACAACAATGAAGCAA
ATTTTATCAGGCGAAACAGTTGTATTGCACTCATATGATTACAAGACAAACTCTAAATCT
ACTGAAAAGATTACAATTTATCCAGCTGATGTTGTACTTTTTGAAGGAATTTTGGTCTTT
TATTTTCCGTCTATTCGCGATCTCTTTCATATGAAGTTGTTTGTTGATACAGATTCGTAA
GTATATGAACTTGCAAGAAGAGTACCTCGTGACATAAAAGAAAGAGGAAGAGATCTTGAT
CAGGTTTTAAATCAGTATATGATGTTTGTTAAGCCAGCTTTTGAAGAGTTTTGTTCACCA
ACAAAAAAATATGCTGATGTTATTATCCCACGAGGGGTCGACAATTTGGTTGCAATAAGC
CTCATTGTGCATCACATCAACGAAATTTTACACCCGACTACTAATTCTACCACTACGAAT
GGAACTAATGGTCATTACACAAATGGATCCTTGTATTTATAATACAATCGATGCTATCAT
ATAATTATTCCATCACATGAATAACAAACATGTTTGTTTATCGATTTTTTTCAATCAGTG
AAAAACTTTCCATACATTAAATTTGCACAAAATTTGTGTATTGCATTCCTTTATTTAGAC
TCGTATGAAGATTAAACAAAATTGGATTACACATTCCTTGAAAAAAATTACAAAAAAATT
GTATACTGGCATGCTAAAAAATGTCTCATCTCTTTTTATACCTCGTAATAATCACTTCCA
CAATTCTATATGTAAAAAAATAGTCTATATCTAAAAGATCATTGTAAAATAAATGTGAAT
TTTTTGTATCAATTTTTATATGATATAACAATTCAGAGCCTTTAAAATGATAAATTTATG
GAGAAAGATATGAAATGAAATAAAAACAAACCATATATCTTAATAATCATGAAAAATCTT
CATTTACT
>g10286.t27 Gene=g10286 Length=161
MNENFFVVPHFFLFKIFICHLSFFSGVAGGTASGKSSVCKRIIEKLGQADINERQRQVVS
ISQDSFYRELTEEEKIKAQKGQFNFDHPQSFDEELMYTTMKQILSGETVVLHSYDYKTNS
KSTEKITIYPADVVLFEGILVFYFPSIRDLFHMKLFVDTDS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10286.t27 | Gene3D | G3DSA:3.40.50.300 | - | 22 | 161 | 5.5E-42 |
| 2 | g10286.t27 | PANTHER | PTHR10285 | URIDINE KINASE | 23 | 161 | 7.8E-57 |
| 3 | g10286.t27 | PANTHER | PTHR10285:SF66 | URIDINE-CYTIDINE KINASE 1 | 23 | 161 | 7.8E-57 |
| 1 | g10286.t27 | Pfam | PF00485 | Phosphoribulokinase / Uridine kinase family | 26 | 160 | 2.1E-23 |
| 10 | g10286.t27 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 11 | - |
| 13 | g10286.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 32 | - |
| 9 | g10286.t27 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 33 | 125 | - |
| 12 | g10286.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 126 | 144 | - |
| 11 | g10286.t27 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 145 | 161 | - |
| 6 | g10286.t27 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 23 | 160 | 1.07E-19 |
| 7 | g10286.t27 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 29 | - |
| 5 | g10286.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 10 | 32 | - |
| 4 | g10286.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 126 | 148 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0019206 | nucleoside kinase activity | MF |
| GO:0016301 | kinase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
