General information for g10303.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as cAMP-dependent protein kinase type I regulatory subunit.
Parent gene
- The parent gene g10303 is located on chr_1: 8816577..8820852 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10303 | g10303.t1 | TTS | g10303.t1 | 8795493 | 8795493 |
| chr_1 | g10303 | g10303.t1 | isoform | g10303.t1 | 8796422 | 8820228 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon1 | 8796422 | 8796553 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS1 | 8796422 | 8796553 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon2 | 8796657 | 8796746 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS2 | 8796657 | 8796746 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon3 | 8796821 | 8797102 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS3 | 8796821 | 8797102 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon4 | 8797160 | 8797252 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS4 | 8797160 | 8797252 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon5 | 8798202 | 8798402 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS5 | 8798202 | 8798402 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon6 | 8798490 | 8798651 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS6 | 8798490 | 8798651 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon7 | 8819858 | 8819958 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS7 | 8819858 | 8819958 |
| chr_1 | g10303 | g10303.t1 | exon | g10303.t1.exon8 | 8820165 | 8820228 |
| chr_1 | g10303 | g10303.t1 | cds | g10303.t1.CDS8 | 8820165 | 8820228 |
| chr_1 | g10303 | g10303.t1 | TSS | g10303.t1 | 8820644 | 8820644 |
Sequences
>g10303.t1 Gene=g10303 Length=1125
ATGAATGTATCAGTATCTGATGAGGATTGTTTAAGGGACTGCGAGCAGTATATATCGACA
CACAGTATTCAAAGAGTTTTAAAAGATGCAATTGTACAACTCTGTGTATTTCGACCCGAG
AATCCAGTGACATTTCTACGACAATACTTTCAAAAATTAGAACGAGAACAAGCCAAATTA
GATGCAAATAAACAAGCAACAAGTCCAGATGAATGTGAGGACTTATCACCAATGCCACCG
ACATCAGCACCACCATCCGTACGTCGACGGGGCGCATTCTCAGCTGAACCCGTAACTGAA
GAAGATGCCACTAGTTATGTTAAAAAAGTAGTACCAAAAGACTATAAAACAATGGCAGCA
CTCTCAAAAGCGATAGCCAAAAACGTACTTTTTGCGCATTTAGACGAAAACGAACGTTCA
GATATTTTTGATGCAATGTTTCCAGTGAATTGCCTGCCTGGCGAGTCTATCATTCAACAA
GGCGACGAGGGTGACAACTTTTATGTAATTGATCAAGGAGAAGTTGAGGTGTTTGTAAAT
TCAGAATTGGTTACAACAATTGGCGAGGGTGGAAGCTTTGGTGAGCTCGCGCTCATTTAT
GGAACACCTCGTGCTGCAACTGTACGTGCCAAAACTGATGTAAAATTATGGGGAATCGAT
CGCGATTCATATCGTAGGATTCTTATGGGCTCAACAATTCGTAAACGCAAGATGTATGAT
GAATTTTTGTCACGTGTCTCGATCTTAGAAAGTTTGGATAAATGGGAGAGACTTACAGTA
GCTGATGCTCTTGAGCCAGTCAGTTTTGAAGAGGGTGAGACGATTGTGAAGCAAGGTGAG
CCTGGAAACGATTTCTATATTATTGTTGAAGGTTGTGCTACCGTATTGCAACAAAGAGGA
GAGAATGAAGAGCCATCGGAAGTGGGAAGGCTCGGACCATCCGATTACTTTGGAGAGATT
GCTCTTCTTTTGGATAGACCTCGTGCAGCGACGGTTATCGCACGTACTCCATTAAAATGT
GTCAAACTTGATAGAGCTCGCTTTGAACGAGTATTGGGCTTATGTGCCGACATTCTCAAA
CGCAACATCACTCAATACAATAGCTTTGTATCATTATCAGTTTAG
>g10303.t1 Gene=g10303 Length=374
MNVSVSDEDCLRDCEQYISTHSIQRVLKDAIVQLCVFRPENPVTFLRQYFQKLEREQAKL
DANKQATSPDECEDLSPMPPTSAPPSVRRRGAFSAEPVTEEDATSYVKKVVPKDYKTMAA
LSKAIAKNVLFAHLDENERSDIFDAMFPVNCLPGESIIQQGDEGDNFYVIDQGEVEVFVN
SELVTTIGEGGSFGELALIYGTPRAATVRAKTDVKLWGIDRDSYRRILMGSTIRKRKMYD
EFLSRVSILESLDKWERLTVADALEPVSFEEGETIVKQGEPGNDFYIIVEGCATVLQQRG
ENEEPSEVGRLGPSDYFGEIALLLDRPRAATVIARTPLKCVKLDRARFERVLGLCADILK
RNITQYNSFVSLSV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g10303.t1 | CDD | cd12097 | DD_RI_PKA | 14 | 57 | 9.33783E-23 |
| 20 | g10303.t1 | CDD | cd00038 | CAP_ED | 130 | 239 | 4.86562E-33 |
| 19 | g10303.t1 | CDD | cd00038 | CAP_ED | 248 | 363 | 1.30927E-33 |
| 17 | g10303.t1 | Coils | Coil | Coil | 46 | 66 | - |
| 14 | g10303.t1 | Gene3D | G3DSA:1.20.890.10 | - | 12 | 68 | 2.5E-24 |
| 16 | g10303.t1 | Gene3D | G3DSA:2.60.120.10 | Jelly Rolls | 98 | 230 | 3.7E-53 |
| 15 | g10303.t1 | Gene3D | G3DSA:2.60.120.10 | Jelly Rolls | 231 | 371 | 2.2E-57 |
| 29 | g10303.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 60 | 96 | - |
| 4 | g10303.t1 | PANTHER | PTHR11635 | CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN | 7 | 374 | 2.2E-189 |
| 5 | g10303.t1 | PANTHER | PTHR11635:SF126 | CAMP-DEPENDENT PROTEIN KINASE TYPE I-BETA REGULATORY SUBUNIT | 7 | 374 | 2.2E-189 |
| 18 | g10303.t1 | PIRSF | PIRSF000548 | PK_regulatory | 3 | 374 | 3.5E-121 |
| 10 | g10303.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 151 | 165 | 5.1E-28 |
| 6 | g10303.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 166 | 180 | 5.1E-28 |
| 9 | g10303.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 315 | 324 | 5.1E-28 |
| 7 | g10303.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 327 | 338 | 5.1E-28 |
| 8 | g10303.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 348 | 360 | 5.1E-28 |
| 1 | g10303.t1 | Pfam | PF02197 | Regulatory subunit of type II PKA R-subunit | 21 | 58 | 7.8E-14 |
| 3 | g10303.t1 | Pfam | PF00027 | Cyclic nucleotide-binding domain | 150 | 229 | 1.9E-20 |
| 2 | g10303.t1 | Pfam | PF00027 | Cyclic nucleotide-binding domain | 267 | 352 | 3.1E-22 |
| 28 | g10303.t1 | ProSitePatterns | PS00888 | Cyclic nucleotide-binding domain signature 1. | 157 | 173 | - |
| 25 | g10303.t1 | ProSitePatterns | PS00889 | Cyclic nucleotide-binding domain signature 2. | 193 | 210 | - |
| 27 | g10303.t1 | ProSitePatterns | PS00888 | Cyclic nucleotide-binding domain signature 1. | 275 | 291 | - |
| 26 | g10303.t1 | ProSitePatterns | PS00889 | Cyclic nucleotide-binding domain signature 2. | 317 | 334 | - |
| 31 | g10303.t1 | ProSiteProfiles | PS50042 | cAMP/cGMP binding motif profile. | 130 | 245 | 45.329 |
| 30 | g10303.t1 | ProSiteProfiles | PS50042 | cAMP/cGMP binding motif profile. | 248 | 369 | 41.177 |
| 22 | g10303.t1 | SMART | SM00394 | akam_3 | 21 | 58 | 3.7E-13 |
| 23 | g10303.t1 | SMART | SM00100 | cnmp_10 | 130 | 246 | 8.2E-32 |
| 24 | g10303.t1 | SMART | SM00100 | cnmp_10 | 248 | 367 | 2.9E-35 |
| 13 | g10303.t1 | SUPERFAMILY | SSF47391 | Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit | 11 | 58 | 3.53E-17 |
| 12 | g10303.t1 | SUPERFAMILY | SSF51206 | cAMP-binding domain-like | 112 | 232 | 1.44E-34 |
| 11 | g10303.t1 | SUPERFAMILY | SSF51206 | cAMP-binding domain-like | 241 | 363 | 1.96E-32 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0001932 | regulation of protein phosphorylation | BP |
| GO:0008603 | cAMP-dependent protein kinase regulator activity | MF |
| GO:0005952 | cAMP-dependent protein kinase complex | CC |
KEGG
Orthology
- This transcript has been annotated with K04739 (cAMP-dependent protein kinase regulator)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
