General information for g10304.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein extra-macrochaetae.
Parent gene
- The parent gene g10304 is located on chr_1: 8846496..8847281 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10304 | g10304.t4 | isoform | g10304.t4 | 8842624 | 8847281 |
| chr_1 | g10304 | g10304.t4 | exon | g10304.t4.exon1 | 8842624 | 8843579 |
| chr_1 | g10304 | g10304.t4 | TSS | g10304.t4 | 8842633 | 8842633 |
| chr_1 | g10304 | g10304.t4 | cds | g10304.t4.CDS1 | 8843136 | 8843579 |
| chr_1 | g10304 | g10304.t4 | exon | g10304.t4.exon2 | 8846477 | 8847281 |
| chr_1 | g10304 | g10304.t4 | cds | g10304.t4.CDS2 | 8846477 | 8846518 |
| chr_1 | g10304 | g10304.t4 | TTS | g10304.t4 | 8847286 | 8847286 |
Sequences
>g10304.t4 Gene=g10304 Length=1761
CAAATATTTCATTCTCGTTCAGTCTGCCGTCACGTTCATTTCGTTGCTCATTTTTTGAGT
TTAAATAATAATAAAAGAAAGAAAATTAATTTAACCTGAAGAGGATTTCAAGAGAAAGAG
AGAGAAAATAAGTTTAATTGAGAAAATAAAGAGTATAATCCCGAAAGAGATTTCGCGACA
CGCAAACAAAAGTGAAAAATAAAATAAATAGAAAAATAAATCAACGCATAGAAAAACAAA
AGAAAGAAATTGTGAAAGAAGGAAAAAAAGATTTAATTATTATTAACAAGTGGTTAATTT
CATCAGAGAAGAAACAATACGAAATTAGAAAGTTTATTGACTAAAAAGTTTATTTTTAGT
CACAGACAAATATAAAATAAAAAGTTCAAAAAGATTAAGTGAAAATAAAAATAAATCCGC
GTTTGGATTAAGCCGGATTAGCAACAACAAATTAATAATTTTTCTTGAAGTGTAAGGAAA
CATAAAAAGCCGTCAAAATTCAGCAAAGAAAGATGAAAGCAATCACAGCCGTCTGCTCAA
CTGGCGCTTCAGTACCATCAATTGCCAGTGGACGTGTTTCACGTCATCGCGATGGCGAAA
ATGCAGAAATTCAAATGTACATAAGCAAACTCAAAGATCTCGTACCCTTCATGCCAAAGA
ATCGAAAATTATCGAAATTAGAGGTCATCCATAACGTCATTCAGTACATTTGTGATTTAC
AAACGGCACTCGATACTCATCCAGCTGTCAATGAATTTGATGCAGCCGCTGTGCTTGCTG
GTCAAAATCAAATGATCGAACAAGTGAGTCCTCGACAACCACTTCATGTGAGACCTAGTC
CAAACACCATCTTACCTTCAAATAATCATAGCAACACCTCACCCTTATCACAGCAGCCGC
AACAACCACAACAAATTTTCACCACATCCAATGAATATTCATCAATAAATCATAATACGA
AAAACAATCGTGTTAAAGTCAATTTAATGCGCTTTTGATCTCAGAGGAGCCCACAAGAAA
AATAATATATATTGTTTTTGTTTTAAAACTGAAACTGCTGAGGATAAATGTTTATGAATA
ATTGCAACAAACGCATTTCTTCTCAACATCTCTGTATGCAATTTTAAACTATAAATATAC
ATAATAATTATAAATATTGAGAACTTACGAGTAAATGCAATTAAACACACATTGAATACA
TCTAGATAGAAGGATTCATTATCTACTGCAAAATGAATCGATGAAAAAAAGACTAAAAAA
GTTGACAGACAGAAGCAGCAGCATACAATAGTAAATTTTTTTCGTGTATAACATTAAATG
ATGAAAAAATCTTTTTTTATTTGATTAATTTAAATAATTAAAGTTCTGTGATCTGTAGAT
TATTATTTTATTTTTAAAAAAATAAATGGATTTAAAGAACATAAAGGTGAAAGATAAAGG
TGAAATGTACAAAAACAACTCACATGTTCAAGCTATGCCGCGAGAAACGCAATGAATAAT
AATAATTTTATTAATGATAAGTTTCAAAATTTCTTAAAAATTGTTTGAATTCTGTAGACT
TTTTGATATATTAAAAATTGCGATCAAAAATTATTTTATTTTACAAGTCATTAAGAAAAG
CATAAGTCGCAAAAGAAATCAACATACACGCATACATTTTTTCTTATCAAACAAATTGTT
TATGTTTTTTTTTAATAATTATGAATGAAACAAAAAAGCTAAAAAAAGTAAATAAAAAAT
GAACAAAAACTAGCTGTAAAA
>g10304.t4 Gene=g10304 Length=161
MKAITAVCSTGASVPSIASGRVSRHRDGENAEIQMYISKLKDLVPFMPKNRKLSKLEVIH
NVIQYICDLQTALDTHPAVNEFDAAAVLAGQNQMIEQVSPRQPLHVRPSPNTILPSNNHS
NTSPLSQQPQQPQQIFTTSNEYSSINHNTKNNRVKVNLMRF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g10304.t4 | CDD | cd19695 | bHLH_dnHLH_EMC_like | 27 | 77 | 5.38121E-23 |
| 5 | g10304.t4 | Gene3D | G3DSA:4.10.280.10 | HLH | 29 | 89 | 1.3E-16 |
| 12 | g10304.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 120 | 141 | - |
| 2 | g10304.t4 | PANTHER | PTHR11723:SF17 | PROTEIN EXTRA-MACROCHAETAE | 1 | 133 | 2.0E-34 |
| 3 | g10304.t4 | PANTHER | PTHR11723 | DNA-BINDING PROTEIN INHIBITOR | 1 | 133 | 2.0E-34 |
| 1 | g10304.t4 | Pfam | PF00010 | Helix-loop-helix DNA-binding domain | 33 | 70 | 5.0E-7 |
| 7 | g10304.t4 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 8 | g10304.t4 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 9 | g10304.t4 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 10 | g10304.t4 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 20 | - |
| 6 | g10304.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 161 | - |
| 13 | g10304.t4 | ProSiteProfiles | PS50888 | Myc-type, basic helix-loop-helix (bHLH) domain profile. | 17 | 69 | 9.102 |
| 4 | g10304.t4 | SUPERFAMILY | SSF47459 | HLH, helix-loop-helix DNA-binding domain | 32 | 81 | 1.31E-9 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0045892 | negative regulation of transcription, DNA-templated | BP |
| GO:0005634 | nucleus | CC |
| GO:0005737 | cytoplasm | CC |
| GO:0046983 | protein dimerization activity | MF |
KEGG
Orthology
- This transcript has been annotated with K17696 (DNA-binding protein inhibitor ID, other)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
