General information for g10321.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phospholipase B1, membrane-associated.
Parent gene
- The parent gene g10321 is located on chr_1: 8914576..8916260 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10321 | g10321.t1 | isoform | g10321.t1 | 8914576 | 8916260 |
| chr_1 | g10321 | g10321.t1 | exon | g10321.t1.exon1 | 8914576 | 8914987 |
| chr_1 | g10321 | g10321.t1 | cds | g10321.t1.CDS1 | 8914576 | 8914987 |
| chr_1 | g10321 | g10321.t1 | exon | g10321.t1.exon2 | 8915118 | 8915548 |
| chr_1 | g10321 | g10321.t1 | cds | g10321.t1.CDS2 | 8915118 | 8915548 |
| chr_1 | g10321 | g10321.t1 | exon | g10321.t1.exon3 | 8915862 | 8916260 |
| chr_1 | g10321 | g10321.t1 | cds | g10321.t1.CDS3 | 8915862 | 8916260 |
| chr_1 | g10321 | g10321.t1 | TSS | g10321.t1 | NA | NA |
| chr_1 | g10321 | g10321.t1 | TTS | g10321.t1 | NA | NA |
Sequences
>g10321.t1 Gene=g10321 Length=1242
ATGAAATTTAAAGATCTCGGTGTAATTCTAATTTTAGCGGTGTATTCTAAATTAATAAGT
GGTCAAACATCTTTTCTCGATAAAAAATTTGGACCAATTTTACGTTTTGGAAGACACACT
TTAGATACAATATTTAAACGAGATGGTTTTGAGAATAACTTGAAAATTAATGTGAAAAAT
AATAAAATTCAACGACAATTTAATAATAATGAGAAGTTTTTCTGTGAAACAGATGGATAT
GGCGCGCGTTCTAAAAGTGTGCCAAATAGCGTTCATAGACTTAGTCCAGGGGACATAGAT
ATTGTTGCAGCAATTGGAGACTCTCTGACTGCAAGTAACGGTGCATTTGCATTAGATGAA
CTGCAAGTTCTTAACAAAGGAAGAGGTGTTTCATGGTCCATTGGAGGCCAAGGTACTTGG
CGTGAATATTTGACATTGCCGAATATATTAAAAGAGTACAATCCAAATCTTTATGGCTAT
TCAATAAATACGTTTTCAAATTCTTTTGATAAATCGTCTAAATTCAATGTTGCTGAAGCA
GGAGCCATGATAGTAGATACAGTTCATCAGGCAAAAAATTTAGTAAAAAGAATGCGAAGT
GATAAAAATGTTGATATGAAAAATCATTGGAAATTAGTGACGTACATGATCGGAGGTAAT
GACTTTTGTCTAGATATATGTTATCATAATGATCAAAACCAAATAATCGAAAATGCTGCA
AATAACTTAATTTTGGCAATGAGAATACTTAAAAAGCACTTGCCGAGGACTCTAGTGAAT
GTCGTTCTCCCTCCAGACGTACAAATTCTATTAAGAATGAAAAACAGACCGAATGAATGC
AAGACTTTGCATTATTTTGAATGTCCGTGCTTATTCAGTTTGAATCACTTTAAAAATCGC
GATAGAACTATCAGCACAATACACATATGGAAAAAAACAATGGAACGAGTAGTTAAGACG
ACACAAGAATTTCACGATTCTGATGATTTTGAAGTGACAATTCATCCATTTCTTGATAAA
GGCGATGTTCCCAAATTTTCTAATGGTGATACCGATTTAAGCTATATGTCAAAAGATTGT
TTTCATTTAAGTCAAAAAGGCCATGCAACAGTTGCAAATGCTCTATGGAACTCAATGTTA
ACGCCAGAGCGTGCTAGGTTGCGATACTTTAAAAATGAATTTGAAGAATTCAAATGCCCA
ACTGCTACACAACCATTCTTACTGACTTCAAAAAACAGTTGA
>g10321.t1 Gene=g10321 Length=413
MKFKDLGVILILAVYSKLISGQTSFLDKKFGPILRFGRHTLDTIFKRDGFENNLKINVKN
NKIQRQFNNNEKFFCETDGYGARSKSVPNSVHRLSPGDIDIVAAIGDSLTASNGAFALDE
LQVLNKGRGVSWSIGGQGTWREYLTLPNILKEYNPNLYGYSINTFSNSFDKSSKFNVAEA
GAMIVDTVHQAKNLVKRMRSDKNVDMKNHWKLVTYMIGGNDFCLDICYHNDQNQIIENAA
NNLILAMRILKKHLPRTLVNVVLPPDVQILLRMKNRPNECKTLHYFECPCLFSLNHFKNR
DRTISTIHIWKKTMERVVKTTQEFHDSDDFEVTIHPFLDKGDVPKFSNGDTDLSYMSKDC
FHLSQKGHATVANALWNSMLTPERARLRYFKNEFEEFKCPTATQPFLLTSKNS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g10321.t1 | CDD | cd01824 | Phospholipase_B_like | 90 | 382 | 2.88107E-116 |
| 6 | g10321.t1 | Gene3D | G3DSA:3.40.50.1110 | - | 89 | 377 | 1.9E-11 |
| 2 | g10321.t1 | PANTHER | PTHR21325 | PHOSPHOLIPASE B, PLB1 | 58 | 413 | 4.0E-116 |
| 3 | g10321.t1 | PANTHER | PTHR21325:SF31 | PHOSPHOLIPASE B1, MEMBRANE-ASSOCIATED | 58 | 413 | 4.0E-116 |
| 1 | g10321.t1 | Pfam | PF00657 | GDSL-like Lipase/Acylhydrolase | 102 | 375 | 1.7E-14 |
| 8 | g10321.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 9 | g10321.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 10 | g10321.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 14 | - |
| 11 | g10321.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 21 | - |
| 7 | g10321.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 413 | - |
| 4 | g10321.t1 | SUPERFAMILY | SSF52266 | SGNH hydrolase | 102 | 380 | 2.33E-14 |
| 5 | g10321.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004620 | phospholipase activity | MF |
| GO:0016788 | hydrolase activity, acting on ester bonds | MF |
KEGG
Orthology
- This transcript has been annotated with K14621 (phospholipase B1, membrane-associated)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
