General information for g10327.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as DNA repair protein Rev1.
Parent gene
- The parent gene g10327 is located on chr_1: 8924430..8925718 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10327 | g10327.t1 | TSS | g10327.t1 | 8923107 | 8923107 |
| chr_1 | g10327 | g10327.t1 | isoform | g10327.t1 | 8923179 | 8925718 |
| chr_1 | g10327 | g10327.t1 | exon | g10327.t1.exon1 | 8923179 | 8923311 |
| chr_1 | g10327 | g10327.t1 | cds | g10327.t1.CDS1 | 8923179 | 8923311 |
| chr_1 | g10327 | g10327.t1 | exon | g10327.t1.exon2 | 8923374 | 8925718 |
| chr_1 | g10327 | g10327.t1 | cds | g10327.t1.CDS2 | 8923374 | 8925718 |
| chr_1 | g10327 | g10327.t1 | TTS | g10327.t1 | 8925795 | 8925795 |
Sequences
>g10327.t1 Gene=g10327 Length=2478
ATGAAAAGAAGGCCAGTTGATGAAGATAATGGATTTGCAGAGCATGGTGGTTATATGTCA
GCAAAAATCAATAAACTTGAAGAGCAATTTTCAATTTTACAAAAAGATATCAAAAAATCT
AATTTATTTGAAGGTGTATCAATATTTGTAAATGGTAGAACAAATCCATCAGCAGATGAA
TTAAAACGTGTAATGATGGAACATGGCGGTATTTTTCATCATTACCAAAGAAGTTTTACA
AGATTTGTAATTGCTAGTAATCTTGCTGATGTAAAAATAAGATCAAATATCACGAAGAAT
ATAATAAGACCTGAATATATAACAGATTGCTTGAAAGCAAACAAATTACTTGATTATTCA
AATTACTTGTTACACACAAACAAAAACTCTCATCAGCCTCAGATTAATTTCAAAAAGGTT
GAATCAAAAACTGAAGAACAGGAAGATGAATATTTTGATTTTAATTTGGATTCATTAAAT
CAGAAAATACAAAATGCACAAAAGGAAGAAAAAACGGGAACAGCGTATGACAAAAATTAT
CTTCAGGAATTTTTAAATAATTCACGTTTGCATTGTATTTCAACGATGGCCAGTAGTTTT
AAATTCTACATCACGGATTTGAGAAAAAAGCATAAGGGAAATTTTCCTGAAAGAGAAATT
CTTAAAACAAAATGTAGTGCAAAAAATTTTCAACCATATAAAAGTGTAATCATGCATATT
GACATGGATTGCTTTTTTGTTTCGGTGGGTTTGAGAAATAAACCACATTTAAAGGGAATA
CCAATTGCTGTTACACATTCAAGAGGTAAAAGTGATAGAGAAGATTTTATGTCATTCTCT
GAAATTGCTTCATGTAGCTATGAAGCACGAGAGAAAGGTTTAAGAAATGGGATGTTTGTG
GGTCAAGCTTTAAAATTTTGTCCTGAATTAAAAACCATTCCTTATGAGTTCGAAGAGTAT
CGTAAAACAGCTTATAAATTGTATGATACTATAGCGAAATATACTCTTGAAATTGAAGCA
GTTAGCTGTGATGAGCTTTATGCAGATTTGACAAGTTTGCTTAATGATTATAAAATCGAT
CTTATGCAGTTTATTTCCATGCTGAGAAATGAGATTTTTGATCAAACACTCTGTACATGT
TCTGTTGGTATCGGTGCAAATCGACTTCAAGCAAGAATGGCAACAAAAAAAGCAAAACCC
AATGGTCAATTTGAAGTTCTTTCACTTGATATATGTGAATATATGAAAGATAAACGCATT
AGAGAATTACCTGGTGTTGGACATTCAACTGAACATACTCTTCAGCAATTAAAGTTAGAA
ACATGTGGTCAATTACAAGAAATGTCTCTAGTTCAACTTCAACATCATTTTGGAAAGAAA
TTTGGTGAATCACTTCATATGATGGCAAAAGGAATTGATGATAAAAAGTTGAATTATGAG
CAGGTTCGTAAATCAGTTAGTGTTGATGTAAATTATGGAATTCGTTTCAATGATGATGAC
GAAGTTAAACCATTTTTGAAACAAATTTGTGAAGAATTGAGCAAAAGATTAAATGAAATT
GAAAAGAAAGGAAAGTGTGTGACACTAAAAATTATGATGCGTGCTAAAAATGCATCAGCA
GAATCAATCAAATTCTTAGGACATGGTGAAGTTGACAAAGTTAGCAAATCAGTTTCTTTA
CATTCATCAACAAGTGATCCATTTATAATATTTCAAAATAGTATAAGTATGCTGACGAGT
CTCAACATACCCGCACATGATTTGAGAGGTATCGGAATTCAGATTTCAAAATTGGATGAT
GATGACAACAAATCGAAAGGGAAGTTAGCAGAAATGTTTAAGAAAATGTCTGAGAAGCAA
GAAGTAAATTTTCAGCGAGTTAAATCAGAAACAAGTGTATCAAAGCCACAAAAAGAAGAA
ATTTCACCTCAAAAGAAAAGAATGAGAACTTTTAAAAAATCAAACTCAAATTCAAATTTA
ACTTCTGTTGCTGACATGTTTGCAGCTAAGAAAACATCATCAACCTCTAAAAGAAAACAA
ATTGATCCAGATATTTTAGCAGAGCTTCCATCAGATATAGTTGAAGAAATTTTAAGAGAT
TATGACATGAATGATGAATATGAAGATAATGAAAGTAATTTTACAGAACAAACAGTTGAA
GTTAAAGAAGAGAATGAAATATCAGATCATAATATTTTTATGAGAAATGATTGGCGTACA
ACAATAATTTCATGGATAGAAGATAATTTAGCTCCAGAACAATGTATGATAGAAACTCTG
AGCAATGATTTGGTGCAATTAATTAAAACAAAGAATCTAGAGTTACCTTTTTTAATAATG
CGATTTTTACATAGACATGCTGAAAATAGCGAGAAAATTGAATGGCAAAAAGCTTACAAT
ACAATACATTTAAATATGCAAAATGAAATGAAAAATTGCTTTGATGGAAAGCAACTTTCT
ACACCGAAAATTTTTTAA
>g10327.t1 Gene=g10327 Length=825
MKRRPVDEDNGFAEHGGYMSAKINKLEEQFSILQKDIKKSNLFEGVSIFVNGRTNPSADE
LKRVMMEHGGIFHHYQRSFTRFVIASNLADVKIRSNITKNIIRPEYITDCLKANKLLDYS
NYLLHTNKNSHQPQINFKKVESKTEEQEDEYFDFNLDSLNQKIQNAQKEEKTGTAYDKNY
LQEFLNNSRLHCISTMASSFKFYITDLRKKHKGNFPEREILKTKCSAKNFQPYKSVIMHI
DMDCFFVSVGLRNKPHLKGIPIAVTHSRGKSDREDFMSFSEIASCSYEAREKGLRNGMFV
GQALKFCPELKTIPYEFEEYRKTAYKLYDTIAKYTLEIEAVSCDELYADLTSLLNDYKID
LMQFISMLRNEIFDQTLCTCSVGIGANRLQARMATKKAKPNGQFEVLSLDICEYMKDKRI
RELPGVGHSTEHTLQQLKLETCGQLQEMSLVQLQHHFGKKFGESLHMMAKGIDDKKLNYE
QVRKSVSVDVNYGIRFNDDDEVKPFLKQICEELSKRLNEIEKKGKCVTLKIMMRAKNASA
ESIKFLGHGEVDKVSKSVSLHSSTSDPFIIFQNSISMLTSLNIPAHDLRGIGIQISKLDD
DDNKSKGKLAEMFKKMSEKQEVNFQRVKSETSVSKPQKEEISPQKKRMRTFKKSNSNSNL
TSVADMFAAKKTSSTSKRKQIDPDILAELPSDIVEEILRDYDMNDEYEDNESNFTEQTVE
VKEENEISDHNIFMRNDWRTTIISWIEDNLAPEQCMIETLSNDLVQLIKTKNLELPFLIM
RFLHRHAENSEKIEWQKAYNTIHLNMQNEMKNCFDGKQLSTPKIF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g10327.t1 | CDD | cd17719 | BRCT_Rev1 | 42 | 124 | 8.53287E-30 |
| 15 | g10327.t1 | Coils | Coil | Coil | 503 | 523 | - |
| 13 | g10327.t1 | Gene3D | G3DSA:3.40.50.10190 | - | 33 | 126 | 5.8E-17 |
| 9 | g10327.t1 | Gene3D | G3DSA:3.30.70.270 | - | 206 | 275 | 9.4E-11 |
| 11 | g10327.t1 | Gene3D | G3DSA:2.30.40.20 | - | 276 | 311 | 4.3E-21 |
| 10 | g10327.t1 | Gene3D | G3DSA:3.30.70.270 | - | 312 | 405 | 8.3E-27 |
| 12 | g10327.t1 | Gene3D | G3DSA:1.10.150.20 | 5’ to 3’ exonuclease | 419 | 472 | 9.8E-16 |
| 14 | g10327.t1 | Gene3D | G3DSA:3.30.1490.100 | - | 482 | 606 | 3.1E-43 |
| 8 | g10327.t1 | Gene3D | G3DSA:1.20.58.1280 | - | 730 | 821 | 2.8E-13 |
| 21 | g10327.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 620 | 655 | - |
| 22 | g10327.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 632 | 647 | - |
| 4 | g10327.t1 | PANTHER | PTHR45990 | DNA REPAIR PROTEIN REV1 | 7 | 710 | 3.5E-212 |
| 18 | g10327.t1 | PIRSF | PIRSF036573 | REV1 | 2 | 151 | 2.3E-31 |
| 17 | g10327.t1 | PIRSF | PIRSF036573 | REV1 | 154 | 734 | 7.4E-180 |
| 16 | g10327.t1 | PIRSF | PIRSF036573 | REV1 | 705 | 819 | 0.84 |
| 2 | g10327.t1 | Pfam | PF16589 | BRCT domain, a BRCA1 C-terminus domain | 39 | 123 | 4.4E-14 |
| 1 | g10327.t1 | Pfam | PF00817 | impB/mucB/samB family | 240 | 396 | 1.1E-39 |
| 3 | g10327.t1 | Pfam | PF11799 | impB/mucB/samB family C-terminal domain | 478 | 605 | 2.5E-14 |
| 23 | g10327.t1 | ProSiteProfiles | PS50172 | BRCT domain profile. | 38 | 124 | 17.54 |
| 24 | g10327.t1 | ProSiteProfiles | PS50173 | UmuC domain profile. | 237 | 427 | 50.347 |
| 20 | g10327.t1 | SMART | SM00292 | BRCT_7 | 40 | 114 | 5.4E-6 |
| 6 | g10327.t1 | SUPERFAMILY | SSF52113 | BRCT domain | 33 | 126 | 2.62E-17 |
| 5 | g10327.t1 | SUPERFAMILY | SSF56672 | DNA/RNA polymerases | 228 | 475 | 1.42E-71 |
| 7 | g10327.t1 | SUPERFAMILY | SSF100879 | Lesion bypass DNA polymerase (Y-family), little finger domain | 482 | 596 | 4.71E-14 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0042276 | error-prone translesion synthesis | BP |
| GO:0006281 | DNA repair | BP |
| GO:0003684 | damaged DNA binding | MF |
| GO:0016779 | nucleotidyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03515 (DNA repair protein REV1 [EC:2.7.7.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
