General information for g10327.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative DNA repair protein Rev1.
Parent gene
- The parent gene g10327 is located on chr_1: 8924430..8925718 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10327 | g10327.t8 | isoform | g10327.t8 | 8924430 | 8925718 |
| chr_1 | g10327 | g10327.t8 | exon | g10327.t8.exon1 | 8924430 | 8925718 |
| chr_1 | g10327 | g10327.t8 | cds | g10327.t8.CDS1 | 8924483 | 8925718 |
| chr_1 | g10327 | g10327.t8 | TTS | g10327.t8 | 8925795 | 8925795 |
| chr_1 | g10327 | g10327.t8 | TSS | g10327.t8 | NA | NA |
Sequences
>g10327.t8 Gene=g10327 Length=1289
AAGCAAAACCCAATGGTCAATTTGAAGTTCTTTCACTTGATATATGTGAATATATGAAAG
ATAAACGCATTAGAGAATTACCTGGTGTTGGACATTCAACTGAACATACTCTTCAGCAAT
TAAAGTTAGAAACATGTGGTCAATTACAAGAAATGTCTCTAGTTCAACTTCAACATCATT
TTGGAAAGAAATTTGGTGAATCACTTCATATGATGGCAAAAGGAATTGATGATAAAAAGT
TGAATTATGAGCAGGTTCGTAAATCAGTTAGTGTTGATGTAAATTATGGAATTCGTTTCA
ATGATGATGACGAAGTTAAACCATTTTTGAAACAAATTTGTGAAGAATTGAGCAAAAGAT
TAAATGAAATTGAAAAGAAAGGAAAGTGTGTGACACTAAAAATTATGATGCGTGCTAAAA
ATGCATCAGCAGAATCAATCAAATTCTTAGGACATGGTGAAGTTGACAAAGTTAGCAAAT
CAGTTTCTTTACATTCATCAACAAGTGATCCATTTATAATATTTCAAAATAGTATAAGTA
TGCTGACGAGTCTCAACATACCCGCACATGATTTGAGAGGTATCGGAATTCAGATTTCAA
AATTGGATGATGATGACAACAAATCGAAAGGGAAGTTAGCAGAAATGTTTAAGAAAATGT
CTGAGAAGCAAGAAGTAAATTTTCAGCGAGTTAAATCAGAAACAAGTGTATCAAAGCCAC
AAAAAGAAGAAATTTCACCTCAAAAGAAAAGAATGAGAACTTTTAAAAAATCAAACTCAA
ATTCAAATTTAACTTCTGTTGCTGACATGTTTGCAGCTAAGAAAACATCATCAACCTCTA
AAAGAAAACAAATTGATCCAGATATTTTAGCAGAGCTTCCATCAGATATAGTTGAAGAAA
TTTTAAGAGATTATGACATGAATGATGAATATGAAGATAATGAAAGTAATTTTACAGAAC
AAACAGTTGAAGTTAAAGAAGAGAATGAAATATCAGATCATAATATTTTTATGAGAAATG
ATTGGCGTACAACAATAATTTCATGGATAGAAGATAATTTAGCTCCAGAACAATGTATGA
TAGAAACTCTGAGCAATGATTTGGTGCAATTAATTAAAACAAAGAATCTAGAGTTACCTT
TTTTAATAATGCGATTTTTACATAGACATGCTGAAAATAGCGAGAAAATTGAATGGCAAA
AAGCTTACAATACAATACATTTAAATATGCAAAATGAAATGAAAAATTGCTTTGATGGAA
AGCAACTTTCTACACCGAAAATTTTTTAA
>g10327.t8 Gene=g10327 Length=411
MKDKRIRELPGVGHSTEHTLQQLKLETCGQLQEMSLVQLQHHFGKKFGESLHMMAKGIDD
KKLNYEQVRKSVSVDVNYGIRFNDDDEVKPFLKQICEELSKRLNEIEKKGKCVTLKIMMR
AKNASAESIKFLGHGEVDKVSKSVSLHSSTSDPFIIFQNSISMLTSLNIPAHDLRGIGIQ
ISKLDDDDNKSKGKLAEMFKKMSEKQEVNFQRVKSETSVSKPQKEEISPQKKRMRTFKKS
NSNSNLTSVADMFAAKKTSSTSKRKQIDPDILAELPSDIVEEILRDYDMNDEYEDNESNF
TEQTVEVKEENEISDHNIFMRNDWRTTIISWIEDNLAPEQCMIETLSNDLVQLIKTKNLE
LPFLIMRFLHRHAENSEKIEWQKAYNTIHLNMQNEMKNCFDGKQLSTPKIF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g10327.t8 | Coils | Coil | Coil | 89 | 109 | - |
| 8 | g10327.t8 | Gene3D | G3DSA:1.10.150.20 | 5’ to 3’ exonuclease | 5 | 58 | 3.9E-16 |
| 9 | g10327.t8 | Gene3D | G3DSA:3.30.1490.100 | - | 68 | 192 | 9.7E-44 |
| 7 | g10327.t8 | Gene3D | G3DSA:1.20.58.1280 | - | 316 | 407 | 9.7E-14 |
| 5 | g10327.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 206 | 241 | - |
| 6 | g10327.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 218 | 233 | - |
| 2 | g10327.t8 | PANTHER | PTHR45990 | DNA REPAIR PROTEIN REV1 | 1 | 296 | 3.2E-72 |
| 1 | g10327.t8 | Pfam | PF11799 | impB/mucB/samB family C-terminal domain | 64 | 191 | 7.4E-15 |
| 3 | g10327.t8 | SUPERFAMILY | SSF56672 | DNA/RNA polymerases | 4 | 63 | 2.33E-11 |
| 4 | g10327.t8 | SUPERFAMILY | SSF100879 | Lesion bypass DNA polymerase (Y-family), little finger domain | 68 | 182 | 1.48E-14 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006281 | DNA repair | BP |
| GO:0003684 | damaged DNA binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
