General information for g10334.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein disulfide-isomerase A3.
Parent gene
- The parent gene g10334 is located on chr_1: 8934649..8935590 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10334 | g10334.t21 | TTS | g10334.t21 | 8933125 | 8933125 |
| chr_1 | g10334 | g10334.t21 | isoform | g10334.t21 | 8933413 | 8935590 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon1 | 8933413 | 8933710 |
| chr_1 | g10334 | g10334.t21 | cds | g10334.t21.CDS1 | 8933413 | 8933710 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon2 | 8933766 | 8933942 |
| chr_1 | g10334 | g10334.t21 | cds | g10334.t21.CDS2 | 8933766 | 8933942 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon3 | 8934000 | 8934282 |
| chr_1 | g10334 | g10334.t21 | cds | g10334.t21.CDS3 | 8934000 | 8934016 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon4 | 8934464 | 8934696 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon5 | 8934759 | 8934836 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon6 | 8934917 | 8934967 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon7 | 8935028 | 8935109 |
| chr_1 | g10334 | g10334.t21 | exon | g10334.t21.exon8 | 8935459 | 8935590 |
| chr_1 | g10334 | g10334.t21 | TSS | g10334.t21 | 8935652 | 8935652 |
Sequences
>g10334.t21 Gene=g10334 Length=1334
ATGAAATTACTTGTTTTTCTGTCATTTTTGTTTGTTGCTGAGTTGGCTCTCTCGGCTGAG
AGTGATGTTCTTGAATTGACTGATTCTGATTTTGAAACTACAATCAAAGAATATCCAACA
GTGTTGGCAATGATTTTCCACGTATACGTATAGCTTAAACCAGAATATGCCAAAGCAGCA
GAATTGATGAGAACTGATGATCCTCCAATTACTCTTGTCAAAGTTGATTGTACAGAAGGC
GGCAAGGATACTTGCAGCAAATTCGGTGTTTCTGGATATCCCACTCTCAAAATTTTCAGA
AACGGTGAAAATCCTCAGGATTATAATGGTCCACGTGAAGCAAGTGGTATTGTGAAATAT
ATGAGAGCACAAGTTGGTCCATCATCAAAGGATCTTCTAACAGTTGAAGCATATGAAAAG
TTCTTGGGTGTTCAAGAGGGTGCCGTAGTTGGATTCTTTGAAAAAGAAACGGATTTGAAG
ACTGTTTTCTTAAAATATGCCGATTTGCAACGTGAGAAACTTCGCTTTGCACATTCAAGT
GATCCAGAAATTCTCAAGAAAGTTGGCGAAACGTATTTGGTGTACGTAGTCGTGATACTA
TTACTGATTTCAACAATCCATTGGTAACTGCATATTATGCTGTTGATTATATTAAAAATG
CAAAAGGCACAAACTACTGGCGCAATCGTGTACTTAAAGTTGCCAAAGAATGGGAAGGTA
AAATCAATTTTGCTATTGCTGCTAAAGATGAATTCCAACATGAATTGAATGAATATGGTT
ACGATTATGTCGGTGATAAACCAATAATTTTGGCTCGTGACGCTCAAAATCAAAAGTTCA
TCATGAAAGATGAGTTTTCTGTTGACAATCTTCAAGCATTTGTCAATGATCTTTATGAAG
GCAATCTGGAGCCTTACGTTAAATCAGAAGCAATTCCAGTCGATCAAGATGGCCCAGTTA
CAGTTGTAGTTGCAAAGAACTTTAATGAGGCTGTTCCAAAAGATAAGGATGTTTTAATCG
AATTCTACGCACCATGGTGTGGTCATTGCAAAAAATTAGCTCCAACATTCGATGAATTAG
GAACAAAATTGAAGGGTGAAGATGTTGTTATTGCCAAAATGGATGCTACTGCAAACGATG
TTCCTCCTCAATTTGATGTTCGCGGATTCCCGACACTCTTCTGGTTGCCTAAGGGCGCTC
ATGATAAACCAGTTAGATACGATGGCGGTCGTGAATTGAACGATTTTATCAGCTACATCA
GTAAACACGCTACAAATGAATTAAAAGGATACGATCGCTCTGGAAACGAAAAGAAACAAA
AGACAGAATTGTAA
>g10334.t21 Gene=g10334 Length=163
MKDEFSVDNLQAFVNDLYEGNLEPYVKSEAIPVDQDGPVTVVVAKNFNEAVPKDKDVLIE
FYAPWCGHCKKLAPTFDELGTKLKGEDVVIAKMDATANDVPPQFDVRGFPTLFWLPKGAH
DKPVRYDGGRELNDFISYISKHATNELKGYDRSGNEKKQKTEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g10334.t21 | CDD | cd02995 | PDI_a_PDI_a’_C | 38 | 139 | 4.79954E-53 |
| 9 | g10334.t21 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 33 | 2.3E-7 |
| 8 | g10334.t21 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 34 | 147 | 4.0E-37 |
| 2 | g10334.t21 | PANTHER | PTHR18929:SF60 | PROTEIN DISULFIDE-ISOMERASE | 1 | 147 | 5.6E-54 |
| 3 | g10334.t21 | PANTHER | PTHR18929 | PROTEIN DISULFIDE ISOMERASE | 1 | 147 | 5.6E-54 |
| 6 | g10334.t21 | PRINTS | PR00421 | Thioredoxin family signature | 57 | 65 | 5.8E-7 |
| 4 | g10334.t21 | PRINTS | PR00421 | Thioredoxin family signature | 65 | 74 | 5.8E-7 |
| 5 | g10334.t21 | PRINTS | PR00421 | Thioredoxin family signature | 105 | 116 | 5.8E-7 |
| 1 | g10334.t21 | Pfam | PF00085 | Thioredoxin | 39 | 140 | 1.2E-28 |
| 11 | g10334.t21 | ProSitePatterns | PS00194 | Thioredoxin family active site. | 58 | 76 | - |
| 13 | g10334.t21 | ProSiteProfiles | PS51352 | Thioredoxin domain profile. | 17 | 144 | 15.679 |
| 7 | g10334.t21 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 4 | 143 | 3.79E-34 |
| 12 | g10334.t21 | TIGRFAM | TIGR01126 | pdi_dom: protein disulfide-isomerase domain | 42 | 142 | 1.8E-35 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003756 | protein disulfide isomerase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
