General information for g10383.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative D(2) dopamine receptor A.
Parent gene
- The parent gene g10383 is located on chr_1: 9353734..9358953 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10383 | g10383.t1 | isoform | g10383.t1 | 9353734 | 9358953 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon1 | 9353734 | 9353917 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS1 | 9353734 | 9353917 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon2 | 9354028 | 9354140 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS2 | 9354028 | 9354140 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon3 | 9354213 | 9354323 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS3 | 9354213 | 9354323 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon4 | 9354518 | 9354821 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS4 | 9354518 | 9354821 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon5 | 9354959 | 9355126 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS5 | 9354959 | 9355126 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon6 | 9355916 | 9356114 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS6 | 9355916 | 9356114 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon7 | 9356180 | 9356404 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS7 | 9356180 | 9356404 |
| chr_1 | g10383 | g10383.t1 | exon | g10383.t1.exon8 | 9358482 | 9358953 |
| chr_1 | g10383 | g10383.t1 | cds | g10383.t1.CDS8 | 9358482 | 9358953 |
| chr_1 | g10383 | g10383.t1 | TSS | g10383.t1 | NA | NA |
| chr_1 | g10383 | g10383.t1 | TTS | g10383.t1 | NA | NA |
Sequences
>g10383.t1 Gene=g10383 Length=1776
ATGAATCAAATGTTAAATTGTTCCAACAATTTTGGAAATCACCACAGTAATCAGACATTC
GAATTGAGTTTCAACGCGAGTGTTGGAAATTATAGTGAAGATATTTTTATAAGTGAAAAT
TCGACACTCATTGAAGATGCAATTCAATGCATATTAAATTTCAATACAAGCACTATTGAA
ATCACAAATCATTTGGTGCAACAATTTAATTTAAATTTATCATCAATAAATGCTACTAAC
TTTGGCATCAATCAATCAGACGATGATAAGGAACCGCATTATGATTGGACTTTTCTTTTT
GCCATTTTCTTCATTATCGCCGGAGGTCTTGGCAATATTCTCGTTTGTTTGGCGGTAGCA
TTAGATAAGAAGCTTCAAAACGTAACAAATTATTTTCTACTTTCATTGGCTGTTGCTGAT
TTATTGGTTAGTCTTTTTGTGATGCCATTGGGAGCAGTTCCTGCATTTTTAGGGTTTTGG
CCTTTAGGATTTACATGGTGTAACATTTATGTGACATGTGATGTGCTAGCTTGTTCGGCG
AGCATTCTTCATATGTGCTTCATTAGTCTAGGCAGATATTTAGGAATAAGAAATCCGCTT
GGATCTCGACAGAGCTCAACAAAACGACTGACGGGCTTTAAAATTGCCCTCGTTTGGCTT
CTGGCAATGTTAATATCTTCCTCAATTACTGTACTAGGTATCATTGATGAGCATAACATA
ATGCCAAAGCCGAGACAATGTGTTATTAACAATCGAACATTCTTCATCTTTGGCTCCCTG
TTTGCATTTTACGTTCCTATGGTTTTAATGGTGATCACATATGCTCTCACAGTACAGTTA
TTGAAAAAGAAGGCTCGTTTTATTGTTGAGCATTCCGAAAGTGAAACGTTTCGAAGACTT
GGTGGTGGTAGGTATTCATCGAAACAGTCAAGCTCAAGTGATCATGAGCGGAATGCTCGC
AAAGAAGAGGCTCGAAATAATACAAACAAAAATATCTCGCATTCACAATCGCAAAATTCA
ATGAACTGGCGAACACATGGGAGCGGTGGAATTTTAAAGGAAAGATCAAATAATATGAAC
ACAAGCACTTCTCATCCAATGCTTAATTTTAGTAACAACAACAATAATAACAATGGCAGT
TCGAGAAATATATTTGCATCATCTGCACGTGGTCAAACGTATGATAAGAGCACACAAACA
CCCGAAAGCATTGAACGTGAAACGAGGCGACATAAGCTTCGATCATTGAAAATAAATTTC
AATAGTGTACCTACTCCATCATTGAATTTTAAATTGGGATTTTTAAATCAACGAAAACGC
ACAAATTTGTCAGCAAATGCTGTGGCTACTGAGCAGAAAGCAACCAAGGTTTTGGGACTT
GTGTTTTTTACATTTGTCCTATGTTGGAGTCCTTTTTTCATTCTGAATATTTTGTTTGCA
ACATGCCCTACATGTGAGGTACCTGATCATATAATTAATATTTGCTTGTGGTTGGGCTAC
GTGTCGTCTACAATCAATCCAATTATTTATACAATATTCAACAAAACTTTTCGAGCAGCA
TTCATACGTTTACTTAAATGTAAATGTCACAAATCCGGTCGTCCGTCTCGATATCGAAGT
GTTACAGATGCCAGAGGGACAATCAGTTTATGCGTTCCATCGGCTTTACCTTTGGCAATC
TCATTGCAAGGTGCTCCTCTATTGACGCCATCATCCGTTCAAACACCATTAAGTGAATTT
CGTACCGGCTTTCAGATTACTGATGAGGATTGCTAA
>g10383.t1 Gene=g10383 Length=591
MNQMLNCSNNFGNHHSNQTFELSFNASVGNYSEDIFISENSTLIEDAIQCILNFNTSTIE
ITNHLVQQFNLNLSSINATNFGINQSDDDKEPHYDWTFLFAIFFIIAGGLGNILVCLAVA
LDKKLQNVTNYFLLSLAVADLLVSLFVMPLGAVPAFLGFWPLGFTWCNIYVTCDVLACSA
SILHMCFISLGRYLGIRNPLGSRQSSTKRLTGFKIALVWLLAMLISSSITVLGIIDEHNI
MPKPRQCVINNRTFFIFGSLFAFYVPMVLMVITYALTVQLLKKKARFIVEHSESETFRRL
GGGRYSSKQSSSSDHERNARKEEARNNTNKNISHSQSQNSMNWRTHGSGGILKERSNNMN
TSTSHPMLNFSNNNNNNNGSSRNIFASSARGQTYDKSTQTPESIERETRRHKLRSLKINF
NSVPTPSLNFKLGFLNQRKRTNLSANAVATEQKATKVLGLVFFTFVLCWSPFFILNILFA
TCPTCEVPDHIINICLWLGYVSSTINPIIYTIFNKTFRAAFIRLLKCKCHKSGRPSRYRS
VTDARGTISLCVPSALPLAISLQGAPLLTPSSVQTPLSEFRTGFQITDEDC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g10383.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 106 | 525 | 3.6E-100 |
| 13 | g10383.t1 | Gene3D | G3DSA:1.10.530.40 | - | 286 | 445 | 3.6E-100 |
| 39 | g10383.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 299 | 343 | - |
| 40 | g10383.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 310 | 328 | - |
| 37 | g10383.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 329 | 343 | - |
| 38 | g10383.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 388 | 409 | - |
| 2 | g10383.t1 | PANTHER | PTHR24247:SF228 | 5-HYDROXYTRYPTAMINE (SEROTONIN) RECEPTOR 2A, ISOFORM B | 85 | 543 | 5.4E-189 |
| 3 | g10383.t1 | PANTHER | PTHR24247 | 5-HYDROXYTRYPTAMINE RECEPTOR | 85 | 543 | 5.4E-189 |
| 7 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 96 | 120 | 8.5E-43 |
| 8 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 129 | 150 | 8.5E-43 |
| 6 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 174 | 196 | 8.5E-43 |
| 4 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 210 | 231 | 8.5E-43 |
| 10 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 255 | 278 | 8.5E-43 |
| 9 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 454 | 478 | 8.5E-43 |
| 5 | g10383.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 492 | 518 | 8.5E-43 |
| 1 | g10383.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 111 | 510 | 1.8E-64 |
| 20 | g10383.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 95 | - |
| 28 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 119 | - |
| 14 | g10383.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 120 | 130 | - |
| 27 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 131 | 157 | - |
| 19 | g10383.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 158 | 168 | - |
| 26 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 169 | 194 | - |
| 16 | g10383.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 195 | 214 | - |
| 25 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 215 | 235 | - |
| 18 | g10383.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 236 | 254 | - |
| 24 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 255 | 276 | - |
| 15 | g10383.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 277 | 456 | - |
| 23 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 457 | 479 | - |
| 21 | g10383.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 480 | 490 | - |
| 22 | g10383.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 491 | 513 | - |
| 17 | g10383.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 514 | 591 | - |
| 41 | g10383.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 111 | 510 | 41.475 |
| 36 | g10383.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 105 | 525 | 6.3E-4 |
| 11 | g10383.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 76 | 540 | 1.01E-73 |
| 30 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 97 | 119 | - |
| 29 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 132 | 154 | - |
| 32 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 169 | 191 | - |
| 35 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 212 | 234 | - |
| 34 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 254 | 276 | - |
| 33 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 459 | 481 | - |
| 31 | g10383.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 491 | 513 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04157 (5-hydroxytryptamine receptor 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed