General information for g10389.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Retinal guanylyl cyclase 2.
Parent gene
- The parent gene g10389 is located on chr_1: 9479326..9481847 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10389 | g10389.t1 | isoform | g10389.t1 | 9471241 | 9481847 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon1 | 9471241 | 9471325 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS1 | 9471241 | 9471325 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon2 | 9474971 | 9475084 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS2 | 9474971 | 9475084 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon3 | 9476310 | 9477183 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS3 | 9476310 | 9477183 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon4 | 9477920 | 9478674 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS4 | 9477920 | 9478674 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon5 | 9478767 | 9478959 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS5 | 9478767 | 9478959 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon6 | 9479231 | 9479326 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS6 | 9479231 | 9479326 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon7 | 9479418 | 9479594 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS7 | 9479418 | 9479594 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon8 | 9479670 | 9479746 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS8 | 9479670 | 9479746 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon9 | 9479803 | 9480093 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS9 | 9479803 | 9480093 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon10 | 9480199 | 9480238 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS10 | 9480199 | 9480238 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon11 | 9480314 | 9480352 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS11 | 9480314 | 9480352 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon12 | 9480503 | 9480694 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS12 | 9480503 | 9480694 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon13 | 9480819 | 9480830 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS13 | 9480819 | 9480830 |
| chr_1 | g10389 | g10389.t1 | exon | g10389.t1.exon14 | 9480893 | 9481847 |
| chr_1 | g10389 | g10389.t1 | cds | g10389.t1.CDS14 | 9480893 | 9481847 |
| chr_1 | g10389 | g10389.t1 | TSS | g10389.t1 | NA | NA |
| chr_1 | g10389 | g10389.t1 | TTS | g10389.t1 | NA | NA |
Sequences
>g10389.t1 Gene=g10389 Length=3900
ATGTTCAGAGCACAAGTTACAAAGTGTCATTTTGAGGCCAAAGAACGAAAGAAAAGAAGA
ATAATGACTGCAAACAAAACATCAAAGCCTAATTGGGGATTGCCATTAGCGATTGCAGCT
AATTTACACACACCATATCGAATAGAAATCGGAATTGAGAGAATTGAAAGGGCAGATATT
AAAGACAAAATCGGAAAAGTTCTTATAATCTGTTCACCACTCAATTCTCTTACAACTGAT
AAAATTGTGCGTAAATTTAAAAATTATCGTTACATCAAGCTGCTACTGATAGACATGATT
GGCAGTGTATTTAGCTTAAATTATAATCATGAATCAAATACGACAACTGACAAAAGTCGT
GACAATCAATTGGTGCTGACAATTCTTACTCAAAAATACAAGTACATCCTCAGTCGAGAA
TATCATCTCGAATTTAGTCGATATTTAAATTTAAAGAGCTCTCATCTCACATTGAATGCA
CATCATAATCATACTCGAGGCAATAACAACAGCGCAATCGTCTGTGATTCAATGCAACTC
CTACACAGTGTGAAACGTAGCGGTAATTATGGCAGAAACATGACATTATGTGAGCAAAAA
ATCAAAGAGCAATGTCACAATCTCAAAAGCAACGAGATCAAATTCCTTTGTGCAAACATA
ACGGAAGAAGGCGAGTACACGTTCAATAAGCAATTAGTGTTCAGTGAGCACTTCAATCTA
TTTGATTTCATCACGTCCATCGTGCGGAATGATGACGGTGATGTTGTTAGAAATGAAAGC
AATCGACATCATCAATCTGATTTTATTGTACTCGACTTCAAATCACTTCTAAATGCGACA
ACATTTGCTGCTGGTTGGCGACCTCTTATGATACTTGAACAAAATCATATTGACAAGAAC
GCCTTCATTCTGCATCATGCACTTTCAGAACGTGATGCCTTCGATGAAATGAAAATGGTA
GATTTATTAAAGTGGAAATGTGGCTCACTATGTTGGGGAATCATTGCCGCCATTTTTCTT
ATCATCATTGTCCTCATTCTTATCATCAGCCTCTTTGCTGGCATTGCAGCAAGAAACTAC
TTTCTTAGAAAACGATTGTCTAAAGGACCCAATAAAGTTGTTCTTGCACCAGCTGACTTT
GTATTTCCAATTGATTCGAGAAGGGTAGATGAAGGTATTGAAGCAATGCTTTGTTGCTGG
CTGCAGCAATTACAAGAATTTGGAGGACCAGAGGTTGATAAGCCAGATTTACTGAAAGGT
TCAATAGGTTCGCTTAAAAATCTTGGTTTACCTGGTGTTGCGTCATCGTCTGCTGGTGTA
TCAAGTGGAAAAGCTGGTTCAGGAACTGGCAGTTTAGTGAAACAAAATAATGCCAACATG
CTTGAATTTAAAGCACGCTATCAAGGCGATCTGGTTCAATTGAAAGAAATCCGAGTTCCA
TCCGGCAATTCCATCGAATTGCGAACAAAAGCAATGGACCATCTTATAATAGCGCACGGG
TTAAGGCACGAGAACATTAATCCCTTAATCGGATTTCTCAGTGATCCAAATCGAACGGCA
TTAGTATTCGAGCATTGCTCTCGTGGATCACTCCAAGATGTGCTCATCATGGATGAAATA
AAACTTGACTGGTCATTTAGACTGAGCTTACTAACAGATCTTGTTCGCGGTATGCGATAT
TTACATTCAGGGCCATTACGTGTTCATGGATCGTTGACATCGCGAAATTGTGTTGTTGAT
GCTCGATGGGTTTTAAAAATCACCGACTATGGCTTGCCAGCATTTTACGAGGCTCAAGGA
CTCACTCCACCACCAAAGTCTGCAAAAGAGCAACTGTGGACTGCTCCTGAGGCGCTGCGT
GGCGCGAAAAATTACCCCAAATGTGGCACCCAAGCAGCTGACGTTTTTTCATTTGGCATT
ATCATGCAAGAGGTTGTCGTGCGAGGCGAACCATATTGTATGCTTTCGTTATCACCCGAA
GAGATTATAGCTAAAATTAAAAAACCACCACCATTAATACGACCCTCAGTATCAAAGGGA
GCAGCACCGCCGGAAGCCATAAATATCATGCGTCAGTGCTGGACGGAAAATCCTGAAGCA
AGACCAGACTTTGAAACCATTGGGGAGAAATTCAAACTTTTAAACCACGGCAGGAAGGTG
AATTTTGTCGATACTATGTTTCAAATGCTCGAAAAATATTCAAACAACTTGGAAGAATTA
ATTAAAGAGCGAACGGAGCAATTAGATATGGAAAGAAAAAAGACTGAACAATTACTTAAC
AGAATGTTGCCAAGCTCTGTCGCTGAGAAACTCAAGATGGGACTCGCTGTTGATCCTGAA
ATATTTTCTGAAGTCACAATTTACTTTTCCGATATTGTGGGCTTTACGACAATTGCTGCT
CATTGTACGCCTGTACAAGTTGTCGACCTACTAAATGATTTATATACATGCTTCGATGCT
ACAATCATGAGCTATCCGAATGTTTACAAAGTGGAAACGATAGGCGATGCGTATATGGTG
GTAGGAGGATTGCCTAATCCGCGACCCGATCACGCAGAACAAATTGCCACGATGGCACTA
GAGCTGCTTCATCAGAGCGGAAACTTTAAAGTTAGACATTTACCCGGTGTGCCACTTCAA
TTGCGCATTGGTCTTCATACAGGTCCTTGCTGTGCTGGTGTTGTGGGATTGACTATGCCA
CGTTACTGTCTCTTTGGTGATACCGTAAATACTGCATCAAGAATGGAATCAACAGGCTCA
TCATGGCGCATTCACATGTCACAAACAACATGTGAGCTTTTAGATAAAGCAGGAAGCTAC
ATCATAGAGCCACGTGGACTGATAGAAATTAAGGGCAAGGGAAAGATGAATACATATTGG
CTACTTGGCAAAAAAGGTTTTGATAAACCATTACCAACTCCACCGCCGATAGGTGAATCT
CATGGTTTGGACGAGTTACTGAAGAGAAATTGTGAAATGGCTGCAGCTAAGGCAACATCA
GTTGATGTAGTGCGAATTGCAAATGCAAATTCAACTCAATCAAGTCATTCGCCATCAGTT
ACAGGAGGCGAATCATTGGATGTAAAAGTAGAAATCACACCAGCACCACCAACACCAATG
AGTGACATTCCAAATTCATTTTCTATTGAATCAACAAGCAGTCTAACAATCTGTTCTGAT
ATTTCATCAAAATCTGTACAATCAACATCACCAAATACTAGAAAATTGTCAGAACTACCG
CCTATGGAGAGTGGTGGCTTTTTAAATCCAATCGCAAATTTTAATAGAATGAATCCATCA
CCACCAAATACAGCATCAGCACGTCTATTCAAAAAACTTGAAGAAATGATTGATTTGTCA
CTGCCCTACAATCACTATCGCTGTCTTAGTCCCAGTGAAACAAACCTATCAAATCCACAC
AATAACGATAAAGCCACGCAGGTCGTGAGCCCAAATTCGAAGCAATTAGACTCGGGAAAG
CCTGGCTCGAGTCGTCTGTTGCGACGTCAATTTAGTCTTGATAAGGATGATCTTAACCAA
AAGCACAATCTTGAGACAATATCATCGCTCATTGAATCATCGAGGCCACAAAATTTCACA
CTTAAATCGCAATATCAACCACTCATATCATCATCAAGTCTGCCTGCATCATCAGCTGCT
GCCATCATGCAATGCAATAAATTACAAAAACATCAGTCAGCAAGTATGACTCAGGATTTA
GAGAAAATTGAAGAAATTAATGTGGACTCATCACCTAATAACTCAACTTCAACTATGAGC
ATCACGACGAGCAATAGCTTTAAGAGGAGTCGACATAATAACAGCTTTAGACATACAACT
TCAACGGCACCACCATCTGCATCCATCAGCAATGATCGAAATGACTTTAAGTTTCATTAA
>g10389.t1 Gene=g10389 Length=1299
MFRAQVTKCHFEAKERKKRRIMTANKTSKPNWGLPLAIAANLHTPYRIEIGIERIERADI
KDKIGKVLIICSPLNSLTTDKIVRKFKNYRYIKLLLIDMIGSVFSLNYNHESNTTTDKSR
DNQLVLTILTQKYKYILSREYHLEFSRYLNLKSSHLTLNAHHNHTRGNNNSAIVCDSMQL
LHSVKRSGNYGRNMTLCEQKIKEQCHNLKSNEIKFLCANITEEGEYTFNKQLVFSEHFNL
FDFITSIVRNDDGDVVRNESNRHHQSDFIVLDFKSLLNATTFAAGWRPLMILEQNHIDKN
AFILHHALSERDAFDEMKMVDLLKWKCGSLCWGIIAAIFLIIIVLILIISLFAGIAARNY
FLRKRLSKGPNKVVLAPADFVFPIDSRRVDEGIEAMLCCWLQQLQEFGGPEVDKPDLLKG
SIGSLKNLGLPGVASSSAGVSSGKAGSGTGSLVKQNNANMLEFKARYQGDLVQLKEIRVP
SGNSIELRTKAMDHLIIAHGLRHENINPLIGFLSDPNRTALVFEHCSRGSLQDVLIMDEI
KLDWSFRLSLLTDLVRGMRYLHSGPLRVHGSLTSRNCVVDARWVLKITDYGLPAFYEAQG
LTPPPKSAKEQLWTAPEALRGAKNYPKCGTQAADVFSFGIIMQEVVVRGEPYCMLSLSPE
EIIAKIKKPPPLIRPSVSKGAAPPEAINIMRQCWTENPEARPDFETIGEKFKLLNHGRKV
NFVDTMFQMLEKYSNNLEELIKERTEQLDMERKKTEQLLNRMLPSSVAEKLKMGLAVDPE
IFSEVTIYFSDIVGFTTIAAHCTPVQVVDLLNDLYTCFDATIMSYPNVYKVETIGDAYMV
VGGLPNPRPDHAEQIATMALELLHQSGNFKVRHLPGVPLQLRIGLHTGPCCAGVVGLTMP
RYCLFGDTVNTASRMESTGSSWRIHMSQTTCELLDKAGSYIIEPRGLIEIKGKGKMNTYW
LLGKKGFDKPLPTPPPIGESHGLDELLKRNCEMAAAKATSVDVVRIANANSTQSSHSPSV
TGGESLDVKVEITPAPPTPMSDIPNSFSIESTSSLTICSDISSKSVQSTSPNTRKLSELP
PMESGGFLNPIANFNRMNPSPPNTASARLFKKLEEMIDLSLPYNHYRCLSPSETNLSNPH
NNDKATQVVSPNSKQLDSGKPGSSRLLRRQFSLDKDDLNQKHNLETISSLIESSRPQNFT
LKSQYQPLISSSSLPASSAAAIMQCNKLQKHQSASMTQDLEKIEEINVDSSPNNSTSTMS
ITTSNSFKRSRHNNSFRHTTSTAPPSASISNDRNDFKFH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g10389.t1 | CDD | cd07302 | CHD | 784 | 961 | 3.47315E-69 |
| 9 | g10389.t1 | Coils | Coil | Coil | 723 | 754 | - |
| 8 | g10389.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 444 | 751 | 1.1E-52 |
| 7 | g10389.t1 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 777 | 965 | 3.0E-76 |
| 21 | g10389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1134 | 1160 | - |
| 22 | g10389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1134 | 1164 | - |
| 20 | g10389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1246 | 1299 | - |
| 19 | g10389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1247 | 1293 | - |
| 3 | g10389.t1 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 354 | 978 | 0.0 |
| 4 | g10389.t1 | PANTHER | PTHR11920:SF462 | GUANYLATE CYCLASE | 354 | 978 | 0.0 |
| 2 | g10389.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 464 | 710 | 4.6E-36 |
| 1 | g10389.t1 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 780 | 962 | 1.3E-63 |
| 11 | g10389.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 90 | - |
| 14 | g10389.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 91 | 108 | - |
| 12 | g10389.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 109 | 331 | - |
| 13 | g10389.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 332 | 357 | - |
| 10 | g10389.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 358 | 1299 | - |
| 18 | g10389.t1 | ProSitePatterns | PS00452 | Guanylate cyclase signature. | 893 | 916 | - |
| 24 | g10389.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 439 | 714 | 26.534 |
| 23 | g10389.t1 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 786 | 916 | 48.506 |
| 17 | g10389.t1 | SMART | SM00044 | cyc_6 | 750 | 944 | 1.9E-98 |
| 5 | g10389.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 468 | 755 | 3.37E-47 |
| 6 | g10389.t1 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 779 | 962 | 3.14E-64 |
| 16 | g10389.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 334 | 356 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016849 | phosphorus-oxygen lyase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
- This transcript has been annotated with K12322 (guanylate cyclase 2F)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed