General information for g10450.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Importin-4.
Parent gene
- The parent gene g10450 is located on chr_1: 9881588..9882368 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10450 | g10450.t1 | TSS | g10450.t1 | 9878794 | 9878794 |
| chr_1 | g10450 | g10450.t1 | isoform | g10450.t1 | 9878900 | 9882368 |
| chr_1 | g10450 | g10450.t1 | exon | g10450.t1.exon1 | 9878900 | 9878956 |
| chr_1 | g10450 | g10450.t1 | cds | g10450.t1.CDS1 | 9878900 | 9878956 |
| chr_1 | g10450 | g10450.t1 | exon | g10450.t1.exon2 | 9879051 | 9881588 |
| chr_1 | g10450 | g10450.t1 | cds | g10450.t1.CDS2 | 9879051 | 9881588 |
| chr_1 | g10450 | g10450.t1 | exon | g10450.t1.exon3 | 9881646 | 9881872 |
| chr_1 | g10450 | g10450.t1 | cds | g10450.t1.CDS3 | 9881646 | 9881872 |
| chr_1 | g10450 | g10450.t1 | exon | g10450.t1.exon4 | 9881942 | 9882368 |
| chr_1 | g10450 | g10450.t1 | cds | g10450.t1.CDS4 | 9881942 | 9882368 |
| chr_1 | g10450 | g10450.t1 | TTS | g10450.t1 | 9882623 | 9882623 |
Sequences
>g10450.t1 Gene=g10450 Length=3249
ATGGAGGCAATGGAAGCTATACTTAATAATCTTCTTAGTGCCGAAAATGTACAAAAGGCA
ACAGAAGAATTGAATGAAGTAATGAAGAGGCCGGAAGCAGTTTCAATGCTTTGTGAAGTT
ACATTTCGCAGTCAAAATGCTCAATTAAGACAATATGGTGCAGTTATTCTTAGAAAAAGA
TTATTAAAATTGAGAAATTGGAACATGATTCCGCCTGAGCAACAAGAAATAATTAAAAAT
GGTATTCTTCAAGCTATTCCTAATGAACCAGAAAAATTTGTTCGTACTAGCATTATTGGA
GTTCTTGGTGGTCTTGTGAATCATGAGTTTCCTAAGAAAAATGAATGGACTCAGTCTGTT
TTAAAATTCATTTTCGAAAGTACTCAATCAAGTGATGCACGTTTCAGTCAATTAGGAAGT
GAAACATTATCGATACTTACTGAAACAGCACCTGATCAATTTGTGCCACATTTGCAAACA
ATCGGTGCTTTTGCAAGCAGTGCTTTGATTGCAGCCGAACAAGCTAACGATATGGGAAAT
GTTGTAATTTTCAATATCATAATGGCTATGTCTCATTTAGTTCCTTTTATACTTGGCAAC
AATTCTGCCGAACATATTTACACAAGTGCAGTTCCTTATGTTGTCAAAACACTTTCGGCT
CTTGCAATTTGTAATGATGATGAAAAGTTTATTGAATGCTTTGATATTCTTGAAAATATG
GCAGATAATGTTCCCAAATTACTTACGAATCATGTCAAATTGTTGATTGAATTTTGTCTG
GAAGTGTCTCGTAATAAAGATATCGATGAAGCAATTCGTGTTAAATCAATAACATTTATC
GGTTGGCTTGTGAGAATTAAAAAGAAGGTGATTCTTAAGCAAAAATTGGTTGAGCCAATT
ATTGCTGTACTTTTTGAATTAATGGCATTACCTAGTGATGATTCCGGAGATGATACTGAA
GAATATTTTGGAAGTAATGAAGTTACAACGCCAATGAACTGTGCAACACAAACACTTGAT
GTGCTTGCATTGAATATTCCACCAAAAAATCTTATTCCACCTCTCTTACAATTACTTGAG
CCTGCTTTGCAATCATCGAATGATCCACTGCGAAAAAAAGCTGCTTATTTATCAATTGCT
GTTATCGCTGAAGGATGTAGTTCTGCTATTTGCAAAAAATACTTGAGACCTCTTTTGGAT
TGCATTAAAACTGGCATCACTGATCAAAATCCAGTTATTCGAAATAGTGCATTGTTTGCT
TTGGGACAATTTTCGGAACATCTGCAGCCTGAAATTTCGCAATTTTCTGAAGAAGTGCTT
CCTATTCTTTTTGATTATTTACAAATGCTTTCAAATCAAATTCGCTCTGGTCAAAAAGAG
CCACAACATATCGATCGAGTGTTTTATGCTGTTGAAACCTTTTGTGAAAATCTTGAAGAT
GCTCTTGTGCCTCATTTACCTATTCTTATGGAACGCTTGTTTGATTGCATGTCTCCAACG
AACTCTGTGCATTTACGTGAGCTTGCATTGAGTTGTATATCAGCTACAGCGAATGCTGCT
AAATCAAATTTGCTACCCTACTTTCCGCGTTTAATTGAAGGTTTAAAAATGTATTTAGTA
AAGACCGAAGATGAAGATATTTGTACATTAAGACCTGCAGCAATTGATACATTGGCAGCA
TTAGTGAGGACAATCGGTAAAGAAAACTTTTTACCACTCGCTATTGATACGATGAACCTT
GGTCTCGCATTAATTGAAGATAAAAATGATCCTGATTTGAAGAGAAGTTGTTACAATTTG
TTTGCAGCAATGGCATCAATCCTTAATGAACAAATCGGATCAGCTTTAGAAAGAATTGTA
ACATCAATGATTGATAGTGTGAAATCAACTGAAAGCATCGTTCTGTCAAGTGGTAAAGAC
TCGGATGATATTGAAGAACAAGAAAATGATGACGATGGCGAATTTGATATTGAAAATTCT
GATGGTGAAGAAGAAGATGAGGATGAAATTTTGGGAGTAGAAAATGCATACATGGAAGAA
AAAGAAGAAGCAATCATTGCATTGAAAGAACTTGCAGAACATACTGGTCCTGCTTTTGCT
CCTTACATTAAAGTTTCATTTGAAGAAATTTATAAATTACTCCCTTATCCTAATGAAGAC
ATTCGTCAATCATCTGTTGAGGCTTTGTGCCAATTCGTTATAGCTTTACATAAATTGCAC
AATGTTCAAGGAGTTAAACATACATTGTCAATCCTTATACCAAAATTAAGCGAAATAATT
CATGTAGATGAAGAAAGAATTGTTGTTATGGCAGCTCTAGAAAGTTTTAATCAAATTTTA
GAAGAATTGGGTAAAGCAGCAGTTGAAGTTGATGGATATAAAGATGCAATTTTTTCATGC
ATTTATGATGTTTTAAATAATAAAGTTCAATGTCAATTCGACGAGCCAGAAGAAGATGAT
GAAGATGAAAGTGAATATGATATTGCAATATTAGAATCAGCTGGTGAAATACTTCCTAAA
TTTGGCAAAACAATGAGCAATCAAGAATTTTACGTCTATTTTGAGAGAGTCTCAGAGTTC
TTTGCTGGAAAAATTCAAAAATCAAAGAGTAGAGATGAACTTTCTCAATCACAACGTGCT
ATGGCAGTAGGAATAGTTTCAGAATGTTTTGAGCCACTAGGTGAATTTTCAGCAAGATAT
TTCGACGCATTGATTCCTCTCTTTCTCGAACTGATAAATGATCAGAGCGATGATGAAGTG
AGAAATAATGCAGTGTACGCGATTGGGGAATTAAGCAAACACAGTGGACAAGCTAGTTTT
AAAGCATATCCACAAATTTTAGCAGCATTATCAAATTTAGTTTCAAAAGAAAGTCATGGT
GGAATTCTCGATAATATTTGTGGTACACTCGCTCGATTGATTATTACAAATAGCACATTG
GTTCCTTTAAAAGATGTTCTCCCAGTTTTTATATCTTATCTTCCACTTCGACAAGACTAT
GTTGAAAATGAGCACGTATTTAAATCACTCGAATTATTATATCGACAAGGAAATGAAGTT
TTGTTGCAATTCCTTGAGCGCGTGATTCTTACAGCATTAACTGTTTTACAAAAAAATCAA
TATAACAACGATGCAGTACGAGAGCACATTTTTCAATTTGTTAAACAAGTTAGGATAGAC
TTTCCGGAAAAATTTAATAATGCTATCAATGTTGATGCAGAAATTTCAAGTTTTGTACAA
ACATTGTAA
>g10450.t1 Gene=g10450 Length=1082
MEAMEAILNNLLSAENVQKATEELNEVMKRPEAVSMLCEVTFRSQNAQLRQYGAVILRKR
LLKLRNWNMIPPEQQEIIKNGILQAIPNEPEKFVRTSIIGVLGGLVNHEFPKKNEWTQSV
LKFIFESTQSSDARFSQLGSETLSILTETAPDQFVPHLQTIGAFASSALIAAEQANDMGN
VVIFNIIMAMSHLVPFILGNNSAEHIYTSAVPYVVKTLSALAICNDDEKFIECFDILENM
ADNVPKLLTNHVKLLIEFCLEVSRNKDIDEAIRVKSITFIGWLVRIKKKVILKQKLVEPI
IAVLFELMALPSDDSGDDTEEYFGSNEVTTPMNCATQTLDVLALNIPPKNLIPPLLQLLE
PALQSSNDPLRKKAAYLSIAVIAEGCSSAICKKYLRPLLDCIKTGITDQNPVIRNSALFA
LGQFSEHLQPEISQFSEEVLPILFDYLQMLSNQIRSGQKEPQHIDRVFYAVETFCENLED
ALVPHLPILMERLFDCMSPTNSVHLRELALSCISATANAAKSNLLPYFPRLIEGLKMYLV
KTEDEDICTLRPAAIDTLAALVRTIGKENFLPLAIDTMNLGLALIEDKNDPDLKRSCYNL
FAAMASILNEQIGSALERIVTSMIDSVKSTESIVLSSGKDSDDIEEQENDDDGEFDIENS
DGEEEDEDEILGVENAYMEEKEEAIIALKELAEHTGPAFAPYIKVSFEEIYKLLPYPNED
IRQSSVEALCQFVIALHKLHNVQGVKHTLSILIPKLSEIIHVDEERIVVMAALESFNQIL
EELGKAAVEVDGYKDAIFSCIYDVLNNKVQCQFDEPEEDDEDESEYDIAILESAGEILPK
FGKTMSNQEFYVYFERVSEFFAGKIQKSKSRDELSQSQRAMAVGIVSECFEPLGEFSARY
FDALIPLFLELINDQSDDEVRNNAVYAIGELSKHSGQASFKAYPQILAALSNLVSKESHG
GILDNICGTLARLIITNSTLVPLKDVLPVFISYLPLRQDYVENEHVFKSLELLYRQGNEV
LLQFLERVILTALTVLQKNQYNNDAVREHIFQFVKQVRIDFPEKFNNAINVDAEISSFVQ
TL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10450.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 635 | 668 | - |
| 7 | g10450.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 643 | 668 | - |
| 3 | g10450.t1 | PANTHER | PTHR10527:SF6 | IMPORTIN-4 | 7 | 1057 | 9.7E-193 |
| 4 | g10450.t1 | PANTHER | PTHR10527 | IMPORTIN BETA | 7 | 1057 | 9.7E-193 |
| 2 | g10450.t1 | Pfam | PF03810 | Importin-beta N-terminal domain | 20 | 86 | 1.4E-7 |
| 1 | g10450.t1 | Pfam | PF02985 | HEAT repeat | 904 | 934 | 1.4E-4 |
| 9 | g10450.t1 | ProSiteProfiles | PS50166 | Importin-beta N-terminal domain profile. | 20 | 88 | 10.595 |
| 10 | g10450.t1 | ProSiteProfiles | PS50077 | HEAT repeat profile. | 904 | 943 | 11.838 |
| 6 | g10450.t1 | SUPERFAMILY | SSF48371 | ARM repeat | 5 | 831 | 2.56E-99 |
| 5 | g10450.t1 | SUPERFAMILY | SSF48371 | ARM repeat | 749 | 1067 | 4.82E-21 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031267 | small GTPase binding | MF |
| GO:0006886 | intracellular protein transport | BP |
| GO:0005515 | protein binding | MF |
| GO:0006606 | protein import into nucleus | BP |
KEGG
Orthology
- This transcript has been annotated with K20221 (importin-4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
