Gene loci information

Transcript annotation

  • This transcript has been annotated as Glutathione synthetase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10478 g10478.t4 isoform g10478.t4 10098324 10101482
chr_1 g10478 g10478.t4 exon g10478.t4.exon1 10098324 10098521
chr_1 g10478 g10478.t4 TTS g10478.t4 10098330 10098330
chr_1 g10478 g10478.t4 cds g10478.t4.CDS1 10098510 10098521
chr_1 g10478 g10478.t4 exon g10478.t4.exon2 10098600 10098752
chr_1 g10478 g10478.t4 cds g10478.t4.CDS2 10098600 10098752
chr_1 g10478 g10478.t4 exon g10478.t4.exon3 10098806 10099504
chr_1 g10478 g10478.t4 cds g10478.t4.CDS3 10098806 10099504
chr_1 g10478 g10478.t4 exon g10478.t4.exon4 10099576 10099618
chr_1 g10478 g10478.t4 cds g10478.t4.CDS4 10099576 10099618
chr_1 g10478 g10478.t4 exon g10478.t4.exon5 10100110 10100237
chr_1 g10478 g10478.t4 cds g10478.t4.CDS5 10100110 10100237
chr_1 g10478 g10478.t4 exon g10478.t4.exon6 10101004 10101216
chr_1 g10478 g10478.t4 cds g10478.t4.CDS6 10101004 10101216
chr_1 g10478 g10478.t4 exon g10478.t4.exon7 10101270 10101330
chr_1 g10478 g10478.t4 cds g10478.t4.CDS7 10101270 10101330
chr_1 g10478 g10478.t4 exon g10478.t4.exon8 10101394 10101482
chr_1 g10478 g10478.t4 cds g10478.t4.CDS8 10101394 10101482
chr_1 g10478 g10478.t4 TSS g10478.t4 10101611 10101611

Sequences

>g10478.t4 Gene=g10478 Length=1584
ATGAGTTATCAAAAAGTTCTACCATCAGCCATTGATTTGCCATTAAATCAAAAAGAACTT
GGTGAAATTATTTCGAAGGCTAAAGATTGGGCATTGATGCATGGGGTCTGTATGCGTTCA
AAGACTAACTTTAGTGCTGATGCATTACAGTTTGCACCATTTGTATTAACTCCATCTTTG
TATCCAAGAAAAGAATTTGAAAAAGCTGTGAATCTCCAAACAGTGTTAAATGAGTTAATG
CATAAAGTTGCTCATGACACTGATTTTTTGAGAGAAACTCTCGCAAGTACTATCCAAGTA
GATGAATTTACCGCTAATTTGTTCAAGATTTACGAAACTGTTTTAAATGAAGGATTTACA
CAGATCCTTAATTTAGGGCTGTTCCGTTCTGATTATTTTAATAACGTTAATAATAAAATA
TTACAAGTGGAATTCAATACAATAGCTTCGTCGTTTGCCGGAATTTCTTCACAGTTTCAG
TCATTTCATAGTTACATATTGAACGAGCTTGGTCATGTCGATAAGACTAAAAATCTGCCA
GAGAATAATGCACTATCTGGATTGTGTTCAGCTATGGTTGAAGCTTGGAATATAATTGGA
AATCAAAAAGCTGCCATTCTATTTGTGATTGAAGATGTTACTTATAATATTTGTGATCAG
CGTTTTCATGAATTTGAAATACGTGAAAAGTATCCACATGTTAAAGTCATTCGTCGGACT
TTGACAGAAATTTTCAATACTGGTGTATTGGGATCTAATAAAGAACTAATTGTCGATGGC
ATTGTTGTAAGTGTTGTATATTTCCGTTCTGGTTATGAGCCAAATCAATATCCATCGAAA
AATGAATGGGATGCAAGACTTATGATTGAGCGATCAAATGCAATAAAGTGTCCTTCTATA
CATTATCATCTTGCCGGAACAAAAAAAGTGCAACAAGCTCTTGCTAAAAAAGGAGTTCTA
AATAGATTTCTTTCAAATGAGACAGATATACAAAAAGTTCAAGAGATTTTTACAGGACTT
TATTCTCTTGATAAAAAAGAAGGCGGAGATGAAGTTGTTGATATGGTACTTAAAAATCCT
GAGAGTTTTGTTATGAAACCGCAACGAGAAGGCGGTGGAAATAATATTTATGGAGTTGAT
ATTCCAGAAAAACTTACAGCTATGAGCGATGACGAACGATCAGCTTATATTGTTATGGAT
CGTATTTTTCCACCAATCTCTAAAAGTTATATGATAAGACCAGGTGGACAAATGCAACCT
GAAATTGTCGAAATGGTGAGTGAGCTTGGGATTTTTGGAGCAATAATTGGAACAAAAGAT
AAAATTTTATACAATCGTCAAGTCGGACACATGCTGAGAACAAAAGTGTCTAACGCTAAT
GAAGGTCTGCTGGTTTAGGTGCTCTTGACAGTCCATATCTCTTTGATTCAGAATAATAAT
AATAATAAATTTTATTGTATTTCTTTTATATGTAAAACATTTTTTAAACATTAATGACTG
CATACAGAAAAACATATATATTTTTAATATGGTGATGGTTGATAAATCAATAAATTTTAT
CAATTTAATAAAATCAAAAATACT

>g10478.t4 Gene=g10478 Length=465
MSYQKVLPSAIDLPLNQKELGEIISKAKDWALMHGVCMRSKTNFSADALQFAPFVLTPSL
YPRKEFEKAVNLQTVLNELMHKVAHDTDFLRETLASTIQVDEFTANLFKIYETVLNEGFT
QILNLGLFRSDYFNNVNNKILQVEFNTIASSFAGISSQFQSFHSYILNELGHVDKTKNLP
ENNALSGLCSAMVEAWNIIGNQKAAILFVIEDVTYNICDQRFHEFEIREKYPHVKVIRRT
LTEIFNTGVLGSNKELIVDGIVVSVVYFRSGYEPNQYPSKNEWDARLMIERSNAIKCPSI
HYHLAGTKKVQQALAKKGVLNRFLSNETDIQKVQEIFTGLYSLDKKEGGDEVVDMVLKNP
ESFVMKPQREGGGNNIYGVDIPEKLTAMSDDERSAYIVMDRIFPPISKSYMIRPGGQMQP
EIVEMVSELGIFGAIIGTKDKILYNRQVGHMLRTKVSNANEGLLV

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g10478.t4 Gene3D G3DSA:3.30.1490.80 - 20 420 0
7 g10478.t4 Gene3D G3DSA:3.30.470.20 - 60 462 0
8 g10478.t4 Gene3D G3DSA:1.10.1080.10 Glutathione Synthetase; Chain A 72 339 0
9 g10478.t4 Gene3D G3DSA:3.40.50.1760 - 182 306 0
11 g10478.t4 Gene3D G3DSA:3.30.1490.50 - 340 406 0
3 g10478.t4 PANTHER PTHR11130:SF0 GLUTATHIONE SYNTHETASE 11 462 0
4 g10478.t4 PANTHER PTHR11130 GLUTATHIONE SYNTHETASE 11 462 0
13 g10478.t4 PIRSF PIRSF001558 GSHase 2 464 0
1 g10478.t4 Pfam PF03917 Eukaryotic glutathione synthase, ATP binding domain 18 462 0
2 g10478.t4 Pfam PF03199 Eukaryotic glutathione synthase 205 305 0
6 g10478.t4 SUPERFAMILY SSF56059 Glutathione synthetase ATP-binding domain-like 12 462 0
5 g10478.t4 SUPERFAMILY SSF52440 PreATP-grasp domain 203 307 0
12 g10478.t4 TIGRFAM TIGR01986 glut_syn_euk: glutathione synthetase 21 462 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004363 glutathione synthase activity MF
GO:0006750 glutathione biosynthetic process BP
GO:0016874 ligase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values