General information for g10478.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutathione synthetase.
Parent gene
- The parent gene g10478 is located on chr_1: 10098472..10101482 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10478 | g10478.t5 | TTS | g10478.t5 | 10098330 | 10098330 |
| chr_1 | g10478 | g10478.t5 | isoform | g10478.t5 | 10098472 | 10101482 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon1 | 10098472 | 10098530 |
| chr_1 | g10478 | g10478.t5 | cds | g10478.t5.CDS1 | 10098472 | 10098530 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon2 | 10098602 | 10098752 |
| chr_1 | g10478 | g10478.t5 | cds | g10478.t5.CDS2 | 10098602 | 10098752 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon3 | 10098806 | 10099497 |
| chr_1 | g10478 | g10478.t5 | cds | g10478.t5.CDS3 | 10098806 | 10099465 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon4 | 10099572 | 10099618 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon5 | 10100110 | 10100237 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon6 | 10100652 | 10100836 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon7 | 10101004 | 10101216 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon8 | 10101270 | 10101330 |
| chr_1 | g10478 | g10478.t5 | exon | g10478.t5.exon9 | 10101394 | 10101482 |
| chr_1 | g10478 | g10478.t5 | TSS | g10478.t5 | 10101611 | 10101611 |
Sequences
>g10478.t5 Gene=g10478 Length=1625
ATGAGTTATCAAAAAGTTCTACCATCAGCCATTGATTTGCCATTAAATCAAAAAGAACTT
GGTGAAATTATTTCGAAGGCTAAAGATTGGGCATTGATGCATGGGGTCTGTATGCGTTCA
AAGACTAACTTTAGTGCTGATGCATTACAGTTTGCACCATTTGTATTAACTCCATCTTTG
TATCCAAGAAAAGAATTTGAAAAAGCTGTGAATCTCCAAACAGTGTTAAATGAGTTAATG
CATAAAGTTGCTCATGACACTGATTTTTTGAGAGAAACTCTCGCAAGTACTATCCAAGTA
GATGAATTTACCGCTAATTTGTTCAAGATTTACGAAACTGTTTTAAATGAAGGATTTACA
CAGGAAATATCACTTGGTTTAATTCGATCGGATACAATGCTAGAGACAAAGAAAGGCTGT
ATAGAGAAGTGCAAAAATTTAAATCCAAATTGTCCTAAAAGATACTACTGTTGCGTTGCA
ATGGTTGAAATCAATACTATTGCTTCAGGTTTTGGCCATTTGGGACCAGCATCAAGAGCC
ATTCAAAGATCCTTAATTTAGGGCTGTTCCGTTCTGATTATTTTAATAACGTTAATAATA
AAATATTACAAGTGGAATTCAATACAATAGCTTCGTCGTTTGCCGGAATTTCTTCACAGT
TTCAGTCATTTCATAGTTACATATTGAACGAGCTTGGTCATGTCGATAAGACTAAAAATG
TGAAGAATAATGCACTATCTGGATTGTGTTCAGCTATGGTTGAAGCTTGGAATATAATTG
GAAATCAAAAAGCTGCCATTCTATTTGTGATTGAAGATGTTACTTATAATATTTGTGATC
AGCGTTTTCATGAATTTGAAATACGTGAAAAGTATCCACATGTTAAAGTCATTCGTCGGA
CTTTGACAGAAATTTTCAATACTGGTGTATTGGGATCTAATAAAGAACTAATTGTCGATG
GCATTGTTGTAAGTGTTGTATATTTCCGTTCTGGTTATGAGCCAAATCAATATCCATCGA
AAAATGAATGGGATGCAAGACTTATGATTGAGCGATCAAATGCAATAAAGTGTCCTTCTA
TACATTATCATCTTGCCGGAACAAAAAAAGTGCAACAAGCTCTTGCTAAAAAAGGAGTTC
TAAATAGATTTCTTTCAAATGAGACAGATATACAAAAAGTTCAAGAGATTTTTACAGGAC
TTTATTCTCTTGATAAAAAAGAAGGCGGAGATGAAGTTGTTGATATGGTACTTAAAAATC
CTGAGAGTTTTGTTATGAAACCGCAACGAGAAGGCGGTGGAAATAATATTTATGGAGTTG
ATATTCCAGAAAAACTTACAGCTATGAGCGATGACGAACGATCAGCTTATATTGTTATGG
ATCGTATTTTTCCACCAATCTCTAAAAGTTATATGATAAGACCAGGTGGACAAATGCAAC
CTGAAATTGTCGAAATGGTGAGTGAGCTTGGGATTTTTGGAGCAATAATTGGAACAAAAG
ATAAAATTTTATACAATCGTCAAGTCGGACACATGCTGAGAACAAAAGTGTCTAACGCTA
ATGAAGGTGGAGTAGCTGCTGGTTTAGGTGCTCTTGACAGTCCATATCTCTTTGATTCAG
AATAA
>g10478.t5 Gene=g10478 Length=289
MVEAWNIIGNQKAAILFVIEDVTYNICDQRFHEFEIREKYPHVKVIRRTLTEIFNTGVLG
SNKELIVDGIVVSVVYFRSGYEPNQYPSKNEWDARLMIERSNAIKCPSIHYHLAGTKKVQ
QALAKKGVLNRFLSNETDIQKVQEIFTGLYSLDKKEGGDEVVDMVLKNPESFVMKPQREG
GGNNIYGVDIPEKLTAMSDDERSAYIVMDRIFPPISKSYMIRPGGQMQPEIVEMVSELGI
FGAIIGTKDKILYNRQVGHMLRTKVSNANEGGVAAGLGALDSPYLFDSE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10478.t5 | Gene3D | G3DSA:3.40.50.1760 | - | 2 | 105 | 0e+00 |
| 7 | g10478.t5 | Gene3D | G3DSA:1.10.1080.10 | Glutathione Synthetase; Chain A | 106 | 148 | 1e-07 |
| 9 | g10478.t5 | Gene3D | G3DSA:3.30.1490.50 | - | 149 | 214 | 0e+00 |
| 6 | g10478.t5 | Gene3D | G3DSA:3.30.470.20 | - | 215 | 285 | 0e+00 |
| 3 | g10478.t5 | PANTHER | PTHR11130:SF0 | GLUTATHIONE SYNTHETASE | 1 | 285 | 0e+00 |
| 4 | g10478.t5 | PANTHER | PTHR11130 | GLUTATHIONE SYNTHETASE | 1 | 285 | 0e+00 |
| 2 | g10478.t5 | Pfam | PF03199 | Eukaryotic glutathione synthase | 14 | 114 | 0e+00 |
| 1 | g10478.t5 | Pfam | PF03917 | Eukaryotic glutathione synthase, ATP binding domain | 115 | 285 | 0e+00 |
| 5 | g10478.t5 | SUPERFAMILY | SSF56059 | Glutathione synthetase ATP-binding domain-like | 74 | 286 | 0e+00 |
| 10 | g10478.t5 | TIGRFAM | TIGR01986 | glut_syn_euk: glutathione synthetase | 10 | 285 | 0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004363 | glutathione synthase activity | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
| GO:0016874 | ligase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K21456 (glutathione synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
