General information for g10503.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Bumetanide-sensitive sodium-(potassium)-chloride cotransporter.
Parent gene
- The parent gene g10503 is located on chr_1: 10267173..10269166 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10503 | g10503.t3 | TSS | g10503.t3 | 10261751 | 10261751 |
| chr_1 | g10503 | g10503.t3 | isoform | g10503.t3 | 10261826 | 10266168 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon1 | 10261826 | 10261893 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon2 | 10262183 | 10262467 |
| chr_1 | g10503 | g10503.t3 | cds | g10503.t3.CDS1 | 10262298 | 10262467 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon3 | 10262937 | 10263033 |
| chr_1 | g10503 | g10503.t3 | cds | g10503.t3.CDS2 | 10262937 | 10263033 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon4 | 10263112 | 10264041 |
| chr_1 | g10503 | g10503.t3 | cds | g10503.t3.CDS3 | 10263112 | 10264041 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon5 | 10264099 | 10264485 |
| chr_1 | g10503 | g10503.t3 | cds | g10503.t3.CDS4 | 10264099 | 10264485 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon6 | 10264556 | 10265145 |
| chr_1 | g10503 | g10503.t3 | cds | g10503.t3.CDS5 | 10264556 | 10265145 |
| chr_1 | g10503 | g10503.t3 | exon | g10503.t3.exon7 | 10265933 | 10266168 |
| chr_1 | g10503 | g10503.t3 | cds | g10503.t3.CDS6 | 10265933 | 10266167 |
| chr_1 | g10503 | g10503.t3 | TTS | g10503.t3 | NA | NA |
Sequences
>g10503.t3 Gene=g10503 Length=2593
AGTAATTGAAATTCATTTCTAATAGATCAAATCGTGAATTAAATATCAAATAGTTTAATA
ATTAGTGTTTATTGAACTTTATCCGATCTGTATAAACTATTATTTTTATGGAATAAAATC
AAAATGTCATTAAAAGAAATTTAGTAAATTTTTTCAACGAGAATTTGTGAAAAATCAAGA
AGAATGGAAAGATTTAAAGTTACTCGTGTGAATGATGACTTGAATAGTTCTTCAAGTAAT
GGAAATGCTGAAGTTGCACTTAATATCAGTGAATTAAATATGCGTAAAAATTCACATACA
GTTCCAGTTATTTCGCCTGATCGTCGAAAATTTAGCTTTGCACAGTTAACACGTGAAGCT
CTTCCAAGACTCGATAATTACCGTATATCTAAAAGAGCACTCAAACGTCCAAGTTTAGGT
GAATTGCATGGAGAACCTCAGCAAAAAAAAGAACAAACTGCTGGTGATGATCAACCAGTA
AAAACACATGCCCATTTTAAACTTGGTTGGATTCAGGGTGTATTAATTCCTTGTTTATTG
AACATTTGGGGTGTGATGCTCTTCTTACGTTTATCTTGGGTCGTTGGACAATCTGGCGTT
ATTGAGTCACTTATTATCATTGCAATTTCTGCTATAGTTTGTCTGATTACTGTCTCATCA
TTATCAGCAATTAGTACAAATGGAGAAGTGAAAGGAGGCGGCATCTACTATATAATTTCA
AGATCACTCGGTCCTGAATTTGGAGCTTCCGTTGGAGTTGTTTTTGCATTTGCTAATGCA
GTTGCTGCTGCTATGAATACAATCGGATTTTGTGACTCATTGAATGATTTATTGAGAAAT
CATGGCTGGAAAATAATCGATGGTGGAGTGAATGACGTACGAATTATAGGAATGATTGCA
CTTGTTATTATGATTATCATTTGTGCTATAGGAATGGAATGGGAAGTTAAAGCACAAAAC
TTTCTCGTTGTGACTATCATTGCTGCAATTTTGGATTTCTTAATTGGTTCACTTTGGGGA
CCACGTTCAACTGAAGATATTGCAAAAGGGTTCACTGGTTTCTCAATGGAAGTTTTTAAG
AAAAATTTAAAGCCTGATTATCGATTTAGTGAAGGTCTTGATCAAACTTTCTTGACAGTT
TTTGCTATTTATTTTCCATCTGTTACTGGCATCCAAGCTGGTGCAAATATCTGTGGTGAT
CTCAAAGATCCTGCTTCTGCAATACCCAAAGGAACACTCCTTGCACTTTTGATATCTACA
ATTTCTTATGTATGCTTCGCTTTATTTGCTGGTGGTACTGCATTAAGAGACGGCTCAGGT
TTAGTTATGGATGTTGTAAATAAAACTTATCCTTATAATTTTGATTGTGTTGCAAATCAC
ACATGTCAATATGGACTACAAAATAGTTATTCAGTTATGCAACTGATGTCTGTGTGGGGA
GGTCTAATTTATGCAGGCTGCTGGGCAGCAACTCTTAGTACAGCTCTTACTAATCTTTTG
TCTGTTCCACGATTAGTTCAAGCTCTAGGCGTTGATCGCATTTATCCAGGTGTAATTTTC
TTTTCGAAACCATACGGAAAAACGGGTGAACCTTATCGAGGCTATGTCCTTGTCTTTTTT
GTATCGGCAACCTTCCTATTAATCGCTGAATTGAATCTCATTGCACCACTTATTTCAAAT
TTTTATCTTGCTGCTTATGCACTTATCAACTTCTGTACATTCCATGCTGCTTTTGTTCAG
CCTCTAGGATGGCGTCCAACATTTAGATATTACAATGCTTGGCTCAGTCTTTTCGGCTGT
ATCTTGTGTGTCGTTATAATGTTCTTATTATCATGGGTGTCTTCTTTGATTACAATAACA
ATCATCTTTGCTCTTTATTTGATTGTAATGTATCGAAAACCTGATGTTAATTGGGGAAGC
AGTACACAAGAACAAACTTATAAAACAGCTTTATCAGCAGCATTACGTTTACAGCGTGTC
AATGATCACATTAAAAATTATCATCCACAAATTCTTGTTTTATGTGGCAGTCCTCATACT
CGACCTCCTTTAATTGATTTAGCGCATTCCATTACAAAGCATCATTCATTACTTATTGTT
GGTGATATTGTTAACGGAAAGTTATCACATAGACGACGAAAAGAAAAATATGAAGATGCA
CAGAAGTGGTTGGAAGCAAGAAAGATCAAAGCATTTTACAATCTTATTGATAATGTTGTC
TTTGAAGATGGTGTGAGATCTTTAATTCAAGCTAGCGGTTTTGGCAAATTTCAACCAAAT
ATTGTAATGATGGGATATAAAGCTGATTGGAGGACATGCAACACAAGACAATTGGAAACA
TATTTCAACAGTTTACATACTGCATTTGAATGTCGAATGGCTGTTGCAATTTTGAGCCTT
CCGTCTGGGATAGATTTCTCTCAATATGTTCCAGAATATAAAGAACAAACTCCTGCAGTT
CATTTTGCATCCGAAATTACTAGCTCAAGAGATGAATTTGCTTCAAAGACATTGAAAATG
ATGCATATTGATTCAAATTTGAATTTAGAGGGTATGGTTTCAAGCACAACATCGATCACT
ACACCTCAAAGTG
>g10503.t3 Gene=g10503 Length=803
MERFKVTRVNDDLNSSSSNGNAEVALNISELNMRKNSHTVPVISPDRRKFSFAQLTREAL
PRLDNYRISKRALKRPSLGELHGEPQQKKEQTAGDDQPVKTHAHFKLGWIQGVLIPCLLN
IWGVMLFLRLSWVVGQSGVIESLIIIAISAIVCLITVSSLSAISTNGEVKGGGIYYIISR
SLGPEFGASVGVVFAFANAVAAAMNTIGFCDSLNDLLRNHGWKIIDGGVNDVRIIGMIAL
VIMIIICAIGMEWEVKAQNFLVVTIIAAILDFLIGSLWGPRSTEDIAKGFTGFSMEVFKK
NLKPDYRFSEGLDQTFLTVFAIYFPSVTGIQAGANICGDLKDPASAIPKGTLLALLISTI
SYVCFALFAGGTALRDGSGLVMDVVNKTYPYNFDCVANHTCQYGLQNSYSVMQLMSVWGG
LIYAGCWAATLSTALTNLLSVPRLVQALGVDRIYPGVIFFSKPYGKTGEPYRGYVLVFFV
SATFLLIAELNLIAPLISNFYLAAYALINFCTFHAAFVQPLGWRPTFRYYNAWLSLFGCI
LCVVIMFLLSWVSSLITITIIFALYLIVMYRKPDVNWGSSTQEQTYKTALSAALRLQRVN
DHIKNYHPQILVLCGSPHTRPPLIDLAHSITKHHSLLIVGDIVNGKLSHRRRKEKYEDAQ
KWLEARKIKAFYNLIDNVVFEDGVRSLIQASGFGKFQPNIVMMGYKADWRTCNTRQLETY
FNSLHTAFECRMAVAILSLPSGIDFSQYVPEYKEQTPAVHFASEITSSRDEFASKTLKMM
HIDSNLNLEGMVSSTTSITTPQS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 20 | g10503.t3 | Gene3D | G3DSA:1.20.1740.10 | - | 105 | 592 | 3.1E-28 |
| 3 | g10503.t3 | PANTHER | PTHR11827:SF48 | GH09711P | 44 | 763 | 0.0 |
| 4 | g10503.t3 | PANTHER | PTHR11827 | SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS | 44 | 763 | 0.0 |
| 6 | g10503.t3 | PRINTS | PR01207 | Na-K-Cl co-transporter family signature | 168 | 176 | 7.8E-6 |
| 5 | g10503.t3 | PRINTS | PR01207 | Na-K-Cl co-transporter family signature | 401 | 411 | 7.8E-6 |
| 7 | g10503.t3 | PRINTS | PR01207 | Na-K-Cl co-transporter family signature | 555 | 566 | 7.8E-6 |
| 1 | g10503.t3 | Pfam | PF00324 | Amino acid permease | 112 | 611 | 2.6E-109 |
| 2 | g10503.t3 | Pfam | PF03522 | Solute carrier family 12 | 620 | 772 | 1.4E-51 |
| 24 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 106 | - |
| 35 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 107 | 130 | - |
| 33 | g10503.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 131 | 141 | - |
| 44 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 142 | 165 | - |
| 22 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 166 | 185 | - |
| 39 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 186 | 209 | - |
| 31 | g10503.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 210 | 233 | - |
| 43 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 234 | 253 | - |
| 23 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 254 | 259 | - |
| 36 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 260 | 278 | - |
| 30 | g10503.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 279 | 315 | - |
| 40 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 316 | 338 | - |
| 21 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 339 | 349 | - |
| 38 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 350 | 374 | - |
| 29 | g10503.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 375 | 416 | - |
| 41 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 417 | 439 | - |
| 26 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 440 | 473 | - |
| 42 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 474 | 494 | - |
| 28 | g10503.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 495 | 499 | - |
| 45 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 500 | 518 | - |
| 25 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 519 | 529 | - |
| 34 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 530 | 548 | - |
| 32 | g10503.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 549 | 553 | - |
| 37 | g10503.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 554 | 570 | - |
| 27 | g10503.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 571 | 803 | - |
| 19 | g10503.t3 | SUPERFAMILY | SSF81665 | Calcium ATPase, transmembrane domain M | 94 | 568 | 6.41E-6 |
| 16 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 107 | 128 | - |
| 18 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 143 | 165 | - |
| 15 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 186 | 208 | - |
| 17 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 234 | 253 | - |
| 12 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 260 | 279 | - |
| 10 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 316 | 338 | - |
| 8 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 351 | 373 | - |
| 13 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 417 | 439 | - |
| 9 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 475 | 497 | - |
| 14 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 501 | 523 | - |
| 11 | g10503.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 536 | 567 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0055085 | transmembrane transport | BP |
| GO:0006811 | ion transport | BP |
| GO:0022857 | transmembrane transporter activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
