General information for g10503.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Bumetanide-sensitive sodium-(potassium)-chloride cotransporter.
Parent gene
- The parent gene g10503 is located on chr_1: 10267173..10269166 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10503 | g10503.t5 | isoform | g10503.t5 | 10266379 | 10267173 |
| chr_1 | g10503 | g10503.t5 | exon | g10503.t5.exon1 | 10266379 | 10266664 |
| chr_1 | g10503 | g10503.t5 | cds | g10503.t5.CDS1 | 10266483 | 10266664 |
| chr_1 | g10503 | g10503.t5 | exon | g10503.t5.exon2 | 10266722 | 10267001 |
| chr_1 | g10503 | g10503.t5 | cds | g10503.t5.CDS2 | 10266722 | 10267001 |
| chr_1 | g10503 | g10503.t5 | exon | g10503.t5.exon3 | 10267060 | 10267173 |
| chr_1 | g10503 | g10503.t5 | cds | g10503.t5.CDS3 | 10267060 | 10267173 |
| chr_1 | g10503 | g10503.t5 | TTS | g10503.t5 | 10267294 | 10267294 |
| chr_1 | g10503 | g10503.t5 | TSS | g10503.t5 | NA | NA |
Sequences
>g10503.t5 Gene=g10503 Length=680
CTCCTTTACCGAATCTTCAAAAATTACAAAGTCAAATATCAACAAACGAAATAAATTATG
CAACACAAGGAGGATCGAAAGTTCCTAAAGATGTGCTCGATGCAATGAATGTTTTCAATG
TCAAACAAGAGAAGGGAACAATCGATGTATGGTGGCTTTACGATGATGGCGGTTTAACAA
TGCTTTTACCTTACATCATCTCAACACGTTCTAATTGGGCAAATTGTAAAATTCGAGTTT
TTGCACTCTCAAGTAGACAGCATGAATTAGAAGTTGAAGAAAGAAATATGGCAAATTTAC
TTTCAAAATTACGTATTGACTATTCATCTCTTATAATGTTGCAAGGAATAACTGAAAAAC
CTAAAGATGAGACAATTAAAATGCATCTTAGAATGCTCGATGGCTTCTTAGAGAAAGAAA
ATCAGCATAATGAAGGATCAGTTTCTATGACAGAATTTAAGCAACTTGAAGACAAAACAA
ATCGTCATTTAAAATTGAGAGAAATGCTAAAAGAACACTCGAAGAATGCTAATCTAATTG
TCATGTCTTTACCTATGCCAAGACAAGGTTCTGTCTCAGCACCTCTTTATATGTCTTGGC
TCGACATTCTCACAACAGATATGCCACCCATTTTACTTGTCAGAGGAAATCAAAGTTCTG
TATTAACTTTCTATTCATAA
>g10503.t5 Gene=g10503 Length=191
MNVFNVKQEKGTIDVWWLYDDGGLTMLLPYIISTRSNWANCKIRVFALSSRQHELEVEER
NMANLLSKLRIDYSSLIMLQGITEKPKDETIKMHLRMLDGFLEKENQHNEGSVSMTEFKQ
LEDKTNRHLKLREMLKEHSKNANLIVMSLPMPRQGSVSAPLYMSWLDILTTDMPPILLVR
GNQSSVLTFYS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g10503.t5 | Coils | Coil | Coil | 121 | 141 | - |
| 2 | g10503.t5 | PANTHER | PTHR11827:SF48 | GH09711P | 1 | 190 | 4.6E-88 |
| 3 | g10503.t5 | PANTHER | PTHR11827 | SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS | 1 | 190 | 4.6E-88 |
| 1 | g10503.t5 | Pfam | PF03522 | Solute carrier family 12 | 1 | 191 | 1.5E-94 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006811 | ion transport | BP |
| GO:0022857 | transmembrane transporter activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed