General information for g10549.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein lev-9.
Parent gene
- The parent gene g10549 is located on chr_1: 10444569..10450606 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10549 | g10549.t2 | TSS | g10549.t2 | 10439345 | 10439345 |
| chr_1 | g10549 | g10549.t2 | isoform | g10549.t2 | 10439529 | 10441317 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon1 | 10439529 | 10439586 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS1 | 10439529 | 10439586 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon2 | 10439812 | 10439931 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS2 | 10439812 | 10439931 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon3 | 10439992 | 10440183 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS3 | 10439992 | 10440183 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon4 | 10440243 | 10440577 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS4 | 10440243 | 10440577 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon5 | 10440644 | 10440677 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS5 | 10440644 | 10440677 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon6 | 10440740 | 10440868 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS6 | 10440740 | 10440868 |
| chr_1 | g10549 | g10549.t2 | exon | g10549.t2.exon7 | 10440954 | 10441317 |
| chr_1 | g10549 | g10549.t2 | cds | g10549.t2.CDS7 | 10440954 | 10440964 |
| chr_1 | g10549 | g10549.t2 | TTS | g10549.t2 | NA | NA |
Sequences
>g10549.t2 Gene=g10549 Length=1232
ATGAAAAATTTTCAACTTTTCTGGATCATCTTAGTTTCAGCCATTTTTTTTCAACGAGGC
TATTCAGGACCAACGACAGTAACAGAGGATCCAGATGATTGGGAAGAAGAAGATGATTCT
TCTGAAGCTGATGATGATGGTCGTGTTTATAAAAATCCTAGAAATTTTCCATCACCTGAC
TGTCCTAGAGACGAAGAACAAGCAACCCTCTTAGGGCAAAAGTGTCTAAGAAAATGTTCA
TCAGATGAAGATTGTAAAAGTAAGAAAAAGAAATGCTTATGTGACGGTGCTTGTGGAATG
TCATGTATCAAACCAGATAGAGAATGTCCTGAACTTGAACATCCAGCATTAGGCACTGTA
GTAATGTCAGGACGAACTTTTGGTTCGAGAGCGAGCTATACTTGTAATCACGGTTATCAT
GTCGTGGGATTGCAAACAAGAGTTTGTCAAGCTTATGGCTCCTGGTCTGGCAGTGAGCCA
GCATGCAAGCAAAATATTTATTGCTTAGCACCACCAACAATCGAGCATGCACGACATTCC
GCACTACCAGAACAAGCAACATTTGATTTAGATTCAACGGTTCAGTATCATTGCCATTTG
GGCTATGCAACCGCGGGTTTCCCTCGAGCTAAATGCTTAGCAATCGATGGACAAGCAAGC
TGGTATGGTCCTGATATTTCCTGTGAACCACGTTCATGTGGACAACCAGTCGATCCAGCG
AACTCAGGATGGCATGCGGGCGAATGCTATACATATGGATGTCGGATAACATATCATTGT
GGAGAAGGCTATGAATTAGTTGGAAAACAAGCTGCAGATTGTCAAGCAGATGGAGTTTGG
GCACCAAAAGAAGTTCCAACTTGTGTTTCTTTGACATAAACATGCTACCCCATTCCTTAA
TGTTTTACACACACACTCTACTCTCTCTTTCTATTTTTCTCTCTTAATAAAAACATTTAG
ATGCTTTATAGTCAAATCAGAAGAGATAAATATAAAATATATTCATATGGACATGAAACA
TTGATGTAGATTTGTTTGCTTTTATTGTATGTTTGCAATCAAGTTTATTAGCAATAAATT
AGTTATCGCAATTAAACCAACAAACCACTTTTTTTTCTTTTGCACTTTTTATTTTTATTA
CATGTCACTTTAATTTTTTAAATCATTTGCATGATATATACAAATTCTTTTACTACGATA
TATAAGAGCATTTTGTTTATTATTTTTTCAGT
>g10549.t2 Gene=g10549 Length=292
MKNFQLFWIILVSAIFFQRGYSGPTTVTEDPDDWEEEDDSSEADDDGRVYKNPRNFPSPD
CPRDEEQATLLGQKCLRKCSSDEDCKSKKKKCLCDGACGMSCIKPDRECPELEHPALGTV
VMSGRTFGSRASYTCNHGYHVVGLQTRVCQAYGSWSGSEPACKQNIYCLAPPTIEHARHS
ALPEQATFDLDSTVQYHCHLGYATAGFPRAKCLAIDGQASWYGPDISCEPRSCGQPVDPA
NSGWHAGECYTYGCRITYHCGEGYELVGKQAADCQADGVWAPKEVPTCVSLT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g10549.t2 | CDD | cd00033 | CCP | 109 | 163 | 2.47279E-11 |
| 21 | g10549.t2 | CDD | cd00033 | CCP | 168 | 229 | 1.36617E-5 |
| 20 | g10549.t2 | CDD | cd00033 | CCP | 233 | 289 | 2.5078E-7 |
| 12 | g10549.t2 | Gene3D | G3DSA:2.10.70.10 | Complement Module | 100 | 165 | 5.8E-15 |
| 11 | g10549.t2 | Gene3D | G3DSA:2.10.70.10 | Complement Module | 166 | 227 | 1.7E-11 |
| 13 | g10549.t2 | Gene3D | G3DSA:2.10.70.10 | Complement Module | 228 | 290 | 3.3E-13 |
| 26 | g10549.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 27 | 62 | - |
| 25 | g10549.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 29 | 43 | - |
| 5 | g10549.t2 | PANTHER | PTHR19325 | COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING | 25 | 292 | 2.1E-180 |
| 6 | g10549.t2 | PANTHER | PTHR19325:SF531 | HIG-ANCHORING SCAFFOLD PROTEIN, ISOFORM G | 25 | 292 | 2.1E-180 |
| 1 | g10549.t2 | Pfam | PF00095 | WAP-type (Whey Acidic Protein) ‘four-disulfide core’ | 60 | 105 | 1.4E-5 |
| 4 | g10549.t2 | Pfam | PF00084 | Sushi repeat (SCR repeat) | 109 | 162 | 6.8E-10 |
| 3 | g10549.t2 | Pfam | PF00084 | Sushi repeat (SCR repeat) | 168 | 225 | 5.6E-5 |
| 2 | g10549.t2 | Pfam | PF00084 | Sushi repeat (SCR repeat) | 233 | 288 | 2.3E-9 |
| 15 | g10549.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 16 | g10549.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 17 | g10549.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 17 | - |
| 18 | g10549.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 22 | - |
| 14 | g10549.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 292 | - |
| 27 | g10549.t2 | ProSiteProfiles | PS51390 | WAP-type ‘four-disulfide core’ domain profile. | 53 | 106 | 12.712 |
| 30 | g10549.t2 | ProSiteProfiles | PS50923 | Sushi/CCP/SCR domain profile. | 100 | 164 | 11.358 |
| 28 | g10549.t2 | ProSiteProfiles | PS50923 | Sushi/CCP/SCR domain profile. | 166 | 230 | 8.779 |
| 29 | g10549.t2 | ProSiteProfiles | PS50923 | Sushi/CCP/SCR domain profile. | 231 | 290 | 10.882 |
| 24 | g10549.t2 | SMART | SM00032 | CCP_2 | 109 | 162 | 3.5E-11 |
| 23 | g10549.t2 | SMART | SM00032 | CCP_2 | 168 | 228 | 8.2E-7 |
| 22 | g10549.t2 | SMART | SM00032 | CCP_2 | 233 | 288 | 1.5E-6 |
| 7 | g10549.t2 | SUPERFAMILY | SSF57535 | Complement control module/SCR domain | 109 | 163 | 4.17E-14 |
| 8 | g10549.t2 | SUPERFAMILY | SSF57535 | Complement control module/SCR domain | 166 | 241 | 2.64E-11 |
| 9 | g10549.t2 | SUPERFAMILY | SSF57535 | Complement control module/SCR domain | 228 | 290 | 5.14E-12 |
| 10 | g10549.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005576 | extracellular region | CC |
| GO:0030414 | peptidase inhibitor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
