General information for g10825.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Exostosin-2.
Parent gene
- The parent gene g10825 is located on chr_1: 12153467..12155696 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10825 | g10825.t1 | TTS | g10825.t1 | 12150033 | 12150033 |
| chr_1 | g10825 | g10825.t1 | isoform | g10825.t1 | 12150122 | 12155696 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon1 | 12150122 | 12150481 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS1 | 12150122 | 12150481 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon2 | 12150538 | 12150734 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS2 | 12150538 | 12150734 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon3 | 12150795 | 12150947 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS3 | 12150795 | 12150947 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon4 | 12151030 | 12151848 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS4 | 12151030 | 12151848 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon5 | 12153467 | 12155022 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS5 | 12153467 | 12155022 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon6 | 12155078 | 12155158 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS6 | 12155078 | 12155158 |
| chr_1 | g10825 | g10825.t1 | exon | g10825.t1.exon7 | 12155221 | 12155696 |
| chr_1 | g10825 | g10825.t1 | cds | g10825.t1.CDS7 | 12155221 | 12155696 |
| chr_1 | g10825 | g10825.t1 | TSS | g10825.t1 | 12155782 | 12155782 |
Sequences
>g10825.t1 Gene=g10825 Length=3642
ATGAAAGATAAAAATAGTGCCATAAAAGTTTTCTTAAGTGACAAAGTTGGAATATCTGGT
GGACTTGTTTTAGGATTTATAGCTTTAGTTTTTATTTTTAATTCACAATTGAAGCATCCT
GAAGTTTTCAATTTAAACACTTATAATCATGTTCGCTTCGATAAACTATCAGAATTGAGT
CGTACACGTAATCCTAACTGCACAATATTCGATTGTTTCAATGTATATCGATGTGGAAGT
CATCAGAACAAAATTAGTATTTATATTTATCCTCTTACGGAATATTCTGATGACAACAAA
AAATCAAAAACATTTATAACAAAAGAATTTTATCAAATATTAAAGGCAATAATCAAAAGT
CCTTATTATACTTCAAATCCGCAAGAGGCTTGTTTATTCGTACCAAGTATAGATTTATTG
AACCAAAATCTTATTGATAAGAGTTTAGTTGAAAAAGCACTTGCATCATTAAATTATTGG
AATAATGGACGCAATCATATCTTGTTTAACATGCTCCCTGGAGAAGCTCCCAATTATTCT
ACAGTTTTTGATGTGAAATCAGGAGATGCGATAATTGCTGGAGCAGGGTTTAATACACAA
ACATATCGATATGGATTTGATTTTTCAATTCCGTTCTATAGTCCATTGTTGGACAATTAT
GTGAAGAAGACAGGTGATAAAACAACCAAGAACTATTTTCTAATTTCACCACAACTCAAC
ATGTATGACTATCATCGACGACTTATGCAAGAAATTGTATTAGAAGACTCTAATAATAAT
ATTCTTTTGCTTCAAAAATGTACTCAAGAGATTTTTGAAGATAAAATCTCATCTAATCTA
GAACTCATTGACCATGACATGCGATGCTCATTTCCTGCTTTAACAGCATTAGAGTATCCT
GAAATTTTAGAGCAAGGAATATTTTGTCTTGTTATTAGAGGCGTTAGACTTGCTCAACCA
TCTCTTCTCGAATCATTAGCAACAGGCTGTATTCCTGTTATTGTAGCCGACAACATTATA
CTGCCATTTTCTGAAATTATCGATTGGACATTAGCTTCAATTTCTGTACGTGAGGCTGAT
TTGCATTCAATTTCATCAGTATTAAAAGCAGTTTCACAGACTCGTATTGAAGAGTTACAA
CGACAAGGGCGCTTTTTATATGAAAAATATTTTAAAAATATTGAAACAATTGTTCATTCG
ATGTTGGAAGAATTAAATGATAGAGTATTTCCACATTTATCTCTTGACTACAATAATTGG
AATATAGAAAATCTGCCAAATTCTGCTCAAAATCCACTATTTTTGCCATTAATGGGATCA
AAATCACTTGGTTTCACTGCCGTCATTCTTACATATGACCGCGTTGAGAGTCTCTTTACA
CTTATTCAAAAATTGTCATTTGTACCATCTCTGCAAAAAATACTCGTCATTTGGAATAAT
CAGAAAAAGCAACCACCACCGATGTCATCATTTCCAAAAATAACTAAACCAATAAAAATT
ATTCAGACCAAGGCAAATAAATTGTCAAATCGCTTTTATCCATATCCTGAAATAGAAACA
GAAGCCGTGCTAACAATTGATGATGATATTACTATGCTAACTAGCGATGAACTCGATTTT
GGTTTTGAAGTATGGCGTGAATTTCCTGATCGCATTGTTGGATTTCCATCACGAACACAT
GTATTTGAAAACAATGAGTATAAATATGAATCAGAATGGACGAATTCATTATCAATGGTT
TTAACTGGTGTTGCATTTCATCACAAATATTGGAATTATGCTTATACAACTTCAATGCCT
GGTAATATCAAAGAATATGTTGATGAACATATGAATTGTGAAGATATTGCTATGAATTTT
TTGGTCTCAAATAGGACTGGAAAAGGTCCAATAAAAGTAACACCACGTAAAAAATTTAAA
TGTCATTCGAGACAATGTACAAATGCTGAAATGCTCTCAAGTGATCAAGGACATATGATC
GAGAGAAGTGAATGCATTAATTATTTTACAAAAATATATGGTGTAATGCCTCTAAAAACA
GTTGAATATCGAGCCGATCCTGTTCTTTTCCTCGATAATTTCCCAGAAAAATTAAAACGA
TTTAATGATGTAGAGAATCCCACACTTTCTTCAATTGGTATCAATCATGTTGAAGGTGGT
TGGCCAAAAGATATCAATCGTTTAGATGAAGAGCAAACAGCTCGTTATCGTAAGAAACAA
GAAAAGGATGAATCGTATGTGACCCAAATGAAAGGTCTTATAAAGTCATGCGAAAATGCT
ATTTATCAGAATAATGCTGTTAATTTATATGAAACATATTTCGAAGATATGGAGCAGATA
GATTTAAAAGAGGAATATTCTTCGAAAACTTTGAACATGTTTCGTGATAGAACTAATAGA
AATATTAGAAAAATAGCATGGAATCCAGATGATTCAACAAAATTTACAACAGCACATTGT
GGCAATCAGACATACTTTGATTATTTGAATGATGAGTCTAATACGATGCATATTTGGGAT
ATTGAGTATACTAAGAAGCCTATAAGAACTTTTGATAACCATGCGCAATCATTATGCTTC
GAATATAATCCAAAAGAACATTCGACCTTAGTCACTGGAATGATCACAGGGCAAATAGCT
ATATATGATACAAGACAGAATACTCAATTTCCACAAACAGTTTCATATCGAGAAAATTCA
CATAAAGACCAAGTTAATGCTGTTACATTTTATTGCTCAAAATCAAATATGGAGTTTTTT
AGTGGTAGCTCAAGCGGAGAAATTTATTGGTGGGATTCGAGAATGATTGAAAATGGACCT
ATTGAAGTTCTCGTATTGCAGCCAGAAGTAATGCAAGATAGTATAGCAAAAAAGGAAGAG
AAGGCTTTTGGTGTGGTAGCATTAGAGTATGAGAGCACTATTCCAACAAAATATATTGTA
GGAACAGATCATGGAGTGGTTTATATTTGCAATAAGCGTTTTAAGACACCGGCTGATAGA
ATTTATTCTAAAGTTCAATGCTATAATGGTGTAGTATCAGCAGTTCAAAGAAATCCTTCA
TTTTTGAAATTTTTTTTGAGTGTTGGTGATTTTCAAGCGAAACTTTGGTGTGAAGAGTTG
AAGGAGCAACCAATTTTTTGGACTAAAGAATATTCTTCTGAACTCACATTTGGTTGTTGG
AATGCTATTAGATGTTCGAGCTTTTATTTATGCAGAATGGATGGAGTTTTTGATGCATGG
GATATTATTCATAGAAGCGACAGGCCTGTTTTATCAACTAAAATTTCAGATCACACGCTG
TTAACATGTTCACCTCATAAGGAAGGAAAATTAATCCTTGTTGGCACAAGTGGTGGTGAT
ATACATTTATTACAGCTATCAGAGAACTTAGCCACAACAACAGTTAATGATAGACCACAT
ATGGCAGTCGTCCTCGATAGAGAGACACGAAGAGAAAAGCTTCTTGAAAATAAACAACGT
GAGGTGAAGTTAAAAGAAAAGGAGAAAGAGCGAGAACAAATTCGAATGAAGTTAGGCTTG
CCAGATCCTGAAGAAGTAGAGATGGCGTTAGCTAACGATTTGGCTAAACAAGCAGAAGTA
AATTTTCATTTACAGATTGAGCAGTTGAAATTACATTGTTAA
>g10825.t1 Gene=g10825 Length=1213
MKDKNSAIKVFLSDKVGISGGLVLGFIALVFIFNSQLKHPEVFNLNTYNHVRFDKLSELS
RTRNPNCTIFDCFNVYRCGSHQNKISIYIYPLTEYSDDNKKSKTFITKEFYQILKAIIKS
PYYTSNPQEACLFVPSIDLLNQNLIDKSLVEKALASLNYWNNGRNHILFNMLPGEAPNYS
TVFDVKSGDAIIAGAGFNTQTYRYGFDFSIPFYSPLLDNYVKKTGDKTTKNYFLISPQLN
MYDYHRRLMQEIVLEDSNNNILLLQKCTQEIFEDKISSNLELIDHDMRCSFPALTALEYP
EILEQGIFCLVIRGVRLAQPSLLESLATGCIPVIVADNIILPFSEIIDWTLASISVREAD
LHSISSVLKAVSQTRIEELQRQGRFLYEKYFKNIETIVHSMLEELNDRVFPHLSLDYNNW
NIENLPNSAQNPLFLPLMGSKSLGFTAVILTYDRVESLFTLIQKLSFVPSLQKILVIWNN
QKKQPPPMSSFPKITKPIKIIQTKANKLSNRFYPYPEIETEAVLTIDDDITMLTSDELDF
GFEVWREFPDRIVGFPSRTHVFENNEYKYESEWTNSLSMVLTGVAFHHKYWNYAYTTSMP
GNIKEYVDEHMNCEDIAMNFLVSNRTGKGPIKVTPRKKFKCHSRQCTNAEMLSSDQGHMI
ERSECINYFTKIYGVMPLKTVEYRADPVLFLDNFPEKLKRFNDVENPTLSSIGINHVEGG
WPKDINRLDEEQTARYRKKQEKDESYVTQMKGLIKSCENAIYQNNAVNLYETYFEDMEQI
DLKEEYSSKTLNMFRDRTNRNIRKIAWNPDDSTKFTTAHCGNQTYFDYLNDESNTMHIWD
IEYTKKPIRTFDNHAQSLCFEYNPKEHSTLVTGMITGQIAIYDTRQNTQFPQTVSYRENS
HKDQVNAVTFYCSKSNMEFFSGSSSGEIYWWDSRMIENGPIEVLVLQPEVMQDSIAKKEE
KAFGVVALEYESTIPTKYIVGTDHGVVYICNKRFKTPADRIYSKVQCYNGVVSAVQRNPS
FLKFFLSVGDFQAKLWCEELKEQPIFWTKEYSSELTFGCWNAIRCSSFYLCRMDGVFDAW
DIIHRSDRPVLSTKISDHTLLTCSPHKEGKLILVGTSGGDIHLLQLSENLATTTVNDRPH
MAVVLDRETRREKLLENKQREVKLKEKEKEREQIRMKLGLPDPEEVEMALANDLAKQAEV
NFHLQIEQLKLHC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g10825.t1 | Coils | Coil | Coil | 1151 | 1177 | - |
| 8 | g10825.t1 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 442 | 696 | 1.4E-89 |
| 7 | g10825.t1 | Gene3D | G3DSA:2.130.10.10 | - | 778 | 1168 | 1.7E-18 |
| 3 | g10825.t1 | PANTHER | PTHR11062:SF128 | EXOSTOSIN-2 | 23 | 654 | 6.0E-146 |
| 4 | g10825.t1 | PANTHER | PTHR11062 | EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED | 23 | 654 | 6.0E-146 |
| 2 | g10825.t1 | Pfam | PF03016 | Exostosin family | 82 | 370 | 4.2E-45 |
| 1 | g10825.t1 | Pfam | PF09258 | Glycosyl transferase family 64 domain | 445 | 690 | 2.9E-88 |
| 10 | g10825.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 12 | g10825.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 33 | - |
| 11 | g10825.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 34 | 1213 | - |
| 17 | g10825.t1 | SMART | SM00320 | WD40_4 | 787 | 840 | 270.0 |
| 16 | g10825.t1 | SMART | SM00320 | WD40_4 | 844 | 883 | 5.5 |
| 15 | g10825.t1 | SMART | SM00320 | WD40_4 | 891 | 932 | 1.6 |
| 14 | g10825.t1 | SMART | SM00320 | WD40_4 | 998 | 1037 | 170.0 |
| 18 | g10825.t1 | SMART | SM00320 | WD40_4 | 1088 | 1125 | 210.0 |
| 6 | g10825.t1 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 445 | 691 | 8.44E-26 |
| 5 | g10825.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 798 | 1127 | 2.88E-28 |
| 13 | g10825.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 16 | 33 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006486 | protein glycosylation | BP |
| GO:0016757 | glycosyltransferase activity | MF |
| GO:0005515 | protein binding | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0015020 | glucuronosyltransferase activity | MF |
| GO:0015012 | heparan sulfate proteoglycan biosynthetic process | BP |
| GO:0006024 | glycosaminoglycan biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K02367 (glucuronyl/N-acetylglucosaminyl transferase EXT2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
