Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cytochrome P450 6A1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1088 g1088.t2 TSS g1088.t2 8073659 8073659
chr_3 g1088 g1088.t2 isoform g1088.t2 8073698 8074405
chr_3 g1088 g1088.t2 exon g1088.t2.exon1 8073698 8074405
chr_3 g1088 g1088.t2 cds g1088.t2.CDS1 8073698 8074405
chr_3 g1088 g1088.t2 TTS g1088.t2 NA NA

Sequences

>g1088.t2 Gene=g1088 Length=708
ATGATTTTAGAAATTTTACTTTCAATATTTTTCATTTTTTTACTTTCTCTCTATCGGCAT
TTTAAGAGAAATTATGAATTTTGGAGAAATAGACAGATTCCATTCTTGCAGCCAACATTT
CCAGTTGGTAATATGATGGAAGCTGTCAAAACAAAACAACATTTTGGTTTCGTCATGGAA
GATATTTACCAACACATGAAAAAACGATCAGATGTTGGTGACTATTGCGGCATTTTTTTC
TTTCATCAACCTGTGTTGCTCGTTATGACACCAGAATTTGCTAAAACAGTTTTAGTACGT
GATTTTAATTATTTTGCCGATCGTGGAGTTTATTCAAATGAGGAAGTTGATACATTAAGT
GGAAATTTATTCTTTCTTGAAGGACCAAGATGGAGGTTTTTAAGGCAAAAATTAGCTCCA
ACATTTACTTCAGGAAAACTTAAAAGTATGTTTCCAATAATTCTTGATGTTGGACAGAAA
TTTGTAGAACATTTAAAACCTTATGGAGAGAAGAACATGGAAATTGAAATTTATGATTTA
TTAGCAAGATTTTTAACTGATATTATCAGTTCTTGTGCTTTTGGTTTTGACTCAAATTCA
CTTGAAAATCCAGACAATGAATTTAGAACAATTTCGCAAAAAGGATTAAATTTAAGTAAA
TTCAAAAGCCTAAAATGTTTCCTCTCTATGGTTTTTAGAGAAAAAGCA

>g1088.t2 Gene=g1088 Length=236
MILEILLSIFFIFLLSLYRHFKRNYEFWRNRQIPFLQPTFPVGNMMEAVKTKQHFGFVME
DIYQHMKKRSDVGDYCGIFFFHQPVLLVMTPEFAKTVLVRDFNYFADRGVYSNEEVDTLS
GNLFFLEGPRWRFLRQKLAPTFTSGKLKSMFPIILDVGQKFVEHLKPYGEKNMEIEIYDL
LARFLTDIISSCAFGFDSNSLENPDNEFRTISQKGLNLSKFKSLKCFLSMVFREKA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g1088.t2 Gene3D G3DSA:1.10.630.10 Cytochrome p450 16 234 4.8E-46
2 g1088.t2 PANTHER PTHR24292 CYTOCHROME P450 5 234 9.9E-70
3 g1088.t2 PANTHER PTHR24292:SF95 CYP6A16, ISOFORM B-RELATED 5 234 9.9E-70
5 g1088.t2 PRINTS PR00464 Group II E-class P450 signature 128 148 1.1E-8
4 g1088.t2 PRINTS PR00464 Group II E-class P450 signature 183 201 1.1E-8
1 g1088.t2 Pfam PF00067 Cytochrome P450 60 225 5.5E-26
9 g1088.t2 Phobius SIGNAL_PEPTIDE Signal peptide region 1 19 -
10 g1088.t2 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 4 -
11 g1088.t2 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 5 15 -
12 g1088.t2 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 16 19 -
8 g1088.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 20 236 -
6 g1088.t2 SUPERFAMILY SSF48264 Cytochrome P450 25 224 4.71E-35

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed