Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t13 TSS g10883.t13 12437137 12437137
chr_1 g10883 g10883.t13 isoform g10883.t13 12437248 12440188
chr_1 g10883 g10883.t13 exon g10883.t13.exon1 12437248 12437352
chr_1 g10883 g10883.t13 exon g10883.t13.exon2 12437637 12438215
chr_1 g10883 g10883.t13 cds g10883.t13.CDS1 12437704 12438215
chr_1 g10883 g10883.t13 exon g10883.t13.exon3 12438279 12439524
chr_1 g10883 g10883.t13 cds g10883.t13.CDS2 12438279 12439524
chr_1 g10883 g10883.t13 exon g10883.t13.exon4 12439595 12440188
chr_1 g10883 g10883.t13 cds g10883.t13.CDS3 12439595 12439672
chr_1 g10883 g10883.t13 TTS g10883.t13 12440183 12440183

Sequences

>g10883.t13 Gene=g10883 Length=2524
ATGTTGTCAGCAACAAAGCATTTATTGAAAAACACAAATATCACACAAACTCTAATGAAC
ACCAGAAATCTTTCGAGTTTGATGAAAAATGTGAGTGCTTTCTTGATCCGAAGGTGTAAA
AGGAGCAGTCATTGGTATTGACTTAGGAACAACAAATTCATGTGTGGCTGTTATGGAAGG
AAAACAGGCCAAGGTCATTGAAAACGCAGAAGGTGCAAGAACAACACCATCTCATGTTGC
ATTTACAAAAGACGGAGAACGTTTAGTCGGTATGCCAGCAAAGAGACAAGCTGTGACAAA
TTCTGCAAATACCTTTTATGCTACAAAACGTTTGATTGGACGCCGATTTGAAGATCCCGA
GATTAAGAAAGATATGAAGAACTTGTCATATAAAGTAGTCAAAGCTTCAAATGGTGATGC
ATGGGTTCAATCTTCAGATGGTAAAGTCTATTCGCCTTCACAAATTGGTGCTTTCGTTTT
AATGAAAATGAAGGAAACTGCAGAAGCATATCTTAATCAACCAGTTAAGAATGCAGTTGT
TACTGTTCCTGCATATTTTAATGATTCACAACGTCAAGCAACAAAAGATGCTGGTCAAAT
TTCTGGTCTCAATGTTTTGCGTGTTATTAACGAACCAACAGCTGCTGCTTTGGCTTATGG
CATGGACAAGAGTGAAGATAAAATAATTGCTGTTTATGATCTTGGCGGTGGAACATTCGA
TATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAAGTAAAATCAACAAATGGTGATAC
AATGTTGGGTGGTGAGGATTTCGACAACGCAATTGTCAATTTCTTAGTTGATGAATTCAA
GAAAGAGCAAGGTATTGATATTAGAAAGGATGCAATGGCAATGCAACGTCTTAAAGAAGC
AGCTGAAAAAGCAAAGTGTGAATTGTCATCATCAGTTCAAACTGACATCAATTTGCCATA
TCTTACAATGGACGCAAGTGGACCTAAACACATGAACCTCAAGATGACTCGTTCAAAATT
AGAAAATCTTGTAGGAGATCTCATTAAACGTACAATTGCACCTTGCCAGAAAGCTTTATC
CGATGCCGAAGTATCGAAATCGGATGTCGGTGAAGTTTTGTTAGTCGGTGGTATGACAAG
AATGCCCAAAGTTCAAACAACAGTTCAAGATATTTTCGGTAGACAACCATCACGTTCAGT
TAATCCAGATGAAGCTGTTGCAGTTGGTGCTGCAGTACAAGGTGGTGTTTTGGCTGGAGA
TGTAACTGATGTACTTTTACTTGATGTTACACCATTGTCACTTGGTATTGAAACACTTGG
CGGCGTCTTTACACGTTTGATCACAAGAAACACAACAATTCCAACAAAGAAATCACAAGT
ATTCTCAACAGCAGCTGATGGACAAACACAAGTCGAAATCAAAGTTCACCAGGGTGAACG
TGAAATGGCATCTGATAACAAATTACTTGGAGCTTTTACACTTGTCGGCATTCCACCTGC
TCCACGCGGTGTCCCACAAATCGAAGTTACATTTGATATTGATGCAAACGGTATTGTACA
TGTATCAGCTCGTGACAAGGGAACTGGCAAGGAACAACAAATTGTCATTCAATCATCAGG
TGGTCTCAGTAAAGATGAAATCGAAAACATGATAAAGAATGCCGAACAATATGCACAAGC
TGATAAGACAAAGAAGGATCGAATTGAGGCTATCAATCAGGCTGAGTCAATTGTGCACGA
TACAGAAACAAAAATGGAAGAATTCAAAGATCAATTACCAAAGGAGGAATGCGATAAATT
AAGAGAAGAAATTGCAAAAGTACGAACACTCCTTGCCGATAAAGAAAATGCAGATCCTGA
AGTAGTACGTAAAGAAACTGGAGCTCTACAACAATCATCACTGAAACTCTTCGAAATGGC
ATACAAAAAAATGGCATCAGAATGTGAGAGCTCAGGAAGCAAATCAAGTAGTGAAGAGGG
TGGTGAAAAGAAGGAAGATAAAAATTAAACAAGTTCAGACAACAACTTAAGAGGATAAAT
ATATTTTCCCAGTAATTTTAAAAAGCATTGCATTAAATAAAAATCAGTTTCTGAATTAGA
ATTAAAACAAATACAAAAAAATAGACTTTAAACAAATAAAAAAACTATTAAAGGATCCAA
AAAACAATTATTCAATGAATATCAATGTATTGCAAGGATATAGAGAAAAGAGATATGACA
TCGACAATAATTGGAAAATAATCCTACAAATTTTCATTATTGCACGAGGATTTCAATTAA
ATCGATCAATTTCTCTTCAATCTTAATTTAATATTACCCCAAATGTTTTGTGAACGAAAT
AAAATTCGTATCTACCTTTAGCTAATTAGTTATGATTTTAAGTTAAGGAAAGATTTTTAC
TAAAATTTTCTAAGAAAATATTTGAAAGAAAAATTGTAAAGAATGTGGCTAGAGATAGAT
GTGTTGAATATATAAATAATAATAAATACGAAAAATGAGAATTATAGAAAAATTTGAAAT
GAAA

>g10883.t13 Gene=g10883 Length=611
MEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLIGRRFE
DPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLNQPVKN
AVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVYDLGGG
TFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAMAMQRL
KEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRTIAPCQK
ALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSVNPDEAVAVGAAVQGGVL
AGDVTDVLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAADGQTQVEIKVHQ
GEREMASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVHVSARDKGTGKEQQIVIQ
SSGGLSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHDTETKMEEFKDQLPKEEC
DKLREEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMAYKKMASECESSGSKSSS
EEGGEKKEDKN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
25 g10883.t13 CDD cd11733 HSPA9-like_NBD 1 360 0.0
23 g10883.t13 Coils Coil Coil 237 257 -
22 g10883.t13 Coils Coil Coil 514 534 -
24 g10883.t13 Coils Coil Coil 540 560 -
19 g10883.t13 Gene3D G3DSA:3.30.420.40 - 2 355 3.6E-150
20 g10883.t13 Gene3D G3DSA:3.30.30.30 - 49 101 3.6E-150
18 g10883.t13 Gene3D G3DSA:3.30.420.40 - 167 340 3.6E-150
17 g10883.t13 Gene3D G3DSA:3.90.640.10 Actin; Chain A 206 293 3.6E-150
16 g10883.t13 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 356 484 1.5E-59
21 g10883.t13 Gene3D G3DSA:1.20.1270.10 - 485 588 2.2E-26
4 g10883.t13 Hamap MF_00332 Chaperone protein DnaK [dnaK]. 1 609 32.800129
28 g10883.t13 MobiDBLite mobidb-lite consensus disorder prediction 589 611 -
2 g10883.t13 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 570 3.9E-273
3 g10883.t13 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 1 570 3.9E-273
7 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 13 25 5.2E-72
12 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 36 44 5.2E-72
8 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 121 141 5.2E-72
11 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 180 190 5.2E-72
5 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 312 328 5.2E-72
10 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 343 363 5.2E-72
6 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 366 385 5.2E-72
9 g10883.t13 PRINTS PR00301 70kDa heat shock protein signature 447 463 5.2E-72
1 g10883.t13 Pfam PF00012 Hsp70 protein 1 584 4.3E-259
27 g10883.t13 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 174 187 -
26 g10883.t13 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 315 329 -
15 g10883.t13 SUPERFAMILY SSF53067 Actin-like ATPase domain 3 167 5.18E-52
14 g10883.t13 SUPERFAMILY SSF53067 Actin-like ATPase domain 169 360 4.73E-66
13 g10883.t13 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 362 518 7.72E-63
29 g10883.t13 TIGRFAM TIGR02350 prok_dnaK: chaperone protein DnaK 1 584 1.8E-270

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values