Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t16 TSS g10883.t16 12437137 12437137
chr_1 g10883 g10883.t16 isoform g10883.t16 12437248 12440188
chr_1 g10883 g10883.t16 exon g10883.t16.exon1 12437248 12437337
chr_1 g10883 g10883.t16 cds g10883.t16.CDS1 12437248 12437337
chr_1 g10883 g10883.t16 exon g10883.t16.exon2 12437431 12437468
chr_1 g10883 g10883.t16 cds g10883.t16.CDS2 12437431 12437468
chr_1 g10883 g10883.t16 exon g10883.t16.exon3 12437637 12438215
chr_1 g10883 g10883.t16 cds g10883.t16.CDS3 12437637 12438215
chr_1 g10883 g10883.t16 exon g10883.t16.exon4 12438279 12438864
chr_1 g10883 g10883.t16 cds g10883.t16.CDS4 12438279 12438864
chr_1 g10883 g10883.t16 exon g10883.t16.exon5 12439027 12439524
chr_1 g10883 g10883.t16 cds g10883.t16.CDS5 12439027 12439524
chr_1 g10883 g10883.t16 exon g10883.t16.exon6 12439595 12440188
chr_1 g10883 g10883.t16 cds g10883.t16.CDS6 12439595 12439672
chr_1 g10883 g10883.t16 TTS g10883.t16 12440183 12440183

Sequences

>g10883.t16 Gene=g10883 Length=2385
ATGTTGTCAGCAACAAAGCATTTATTGAAAAACACAAATATCACACAAACTCTAATGAAC
ACCAGAAATCTTTCGAGTTTGATGAAAAATAATTTCAGTCACTTTAACCGATTGCAATGT
AGATATAAATCCGAAGGTGTAAAAGGAGCAGTCATTGGTATTGACTTAGGAACAACAAAT
TCATGTGTGGCTGTTATGGAAGGAAAACAGGCCAAGGTCATTGAAAACGCAGAAGGTGCA
AGAACAACACCATCTCATGTTGCATTTACAAAAGACGGAGAACGTTTAGTCGGTATGCCA
GCAAAGAGACAAGCTGTGACAAATTCTGCAAATACCTTTTATGCTACAAAACGTTTGATT
GGACGCCGATTTGAAGATCCCGAGATTAAGAAAGATATGAAGAACTTGTCATATAAAGTA
GTCAAAGCTTCAAATGGTGATGCATGGGTTCAATCTTCAGATGGTAAAGTCTATTCGCCT
TCACAAATTGGTGCTTTCGTTTTAATGAAAATGAAGGAAACTGCAGAAGCATATCTTAAT
CAACCAGTTAAGAATGCAGTTGTTACTGTTCCTGCATATTTTAATGATTCACAACGTCAA
GCAACAAAAGATGCTGGTCAAATTTCTGGTCTCAATGTTTTGCGTGTTATTAACGAACCA
ACAGCTGCTGCTTTGGCTTATGGCATGGACAAGAGTGAAGATAAAATAATTGCTGTTTAT
GATCTTGGCGGTGGAACATTCGATATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAA
GTAAAATCAACAAATGGTGATACAATGTTGGGTGGTGAGGATTTCGACAACGCAATTGTC
AATTTCTTAGTTGATGAATTCAAGAAAGAGCAAGGTATTGATATTAGAAAGGATGCAATG
GCAATGCAACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTGTGAATTGTCATCATCAGTT
CAAACTGACATCAATTTGCCATATCTTACAATGGACGCAAGTGGACCTAAACACATGAAC
CTCAAGATGACTCGTTCAAAATTAGAAAATCTTGTAGGAGATCTCATTAAACGTACAATT
GCACCTTGCCAGAAAGCTTTATCCGATGCCGAAGTATCGAAATCGGATGTCGGTGAAGTT
TTGTTAGTCGGTGGTATGACAAGAATGCCCAAAGTTCAAACAACAGTTCAAGATATTTTC
GGTAGACAACCATCACGTTCAGTTAATCCAGATGAAGCTGTTGCAGTTGGTGCTGCAGTA
CAAGGTGGTGTTTTGGCTGGAGATGTAACTGATGGTGAACGTGAAATGGCATCTGATAAC
AAATTACTTGGAGCTTTTACACTTGTCGGCATTCCACCTGCTCCACGCGGTGTCCCACAA
ATCGAAGTTACATTTGATATTGATGCAAACGGTATTGTACATGTATCAGCTCGTGACAAG
GGAACTGGCAAGGAACAACAAATTGTCATTCAATCATCAGGTGGTCTCAGTAAAGATGAA
ATCGAAAACATGATAAAGAATGCCGAACAATATGCACAAGCTGATAAGACAAAGAAGGAT
CGAATTGAGGCTATCAATCAGGCTGAGTCAATTGTGCACGATACAGAAACAAAAATGGAA
GAATTCAAAGATCAATTACCAAAGGAGGAATGCGATAAATTAAGAGAAGAAATTGCAAAA
GTACGAACACTCCTTGCCGATAAAGAAAATGCAGATCCTGAAGTAGTACGTAAAGAAACT
GGAGCTCTACAACAATCATCACTGAAACTCTTCGAAATGGCATACAAAAAAATGGCATCA
GAATGTGAGAGCTCAGGAAGCAAATCAAGTAGTGAAGAGGGTGGTGAAAAGAAGGAAGAT
AAAAATTAAACAAGTTCAGACAACAACTTAAGAGGATAAATATATTTTCCCAGTAATTTT
AAAAAGCATTGCATTAAATAAAAATCAGTTTCTGAATTAGAATTAAAACAAATACAAAAA
AATAGACTTTAAACAAATAAAAAAACTATTAAAGGATCCAAAAAACAATTATTCAATGAA
TATCAATGTATTGCAAGGATATAGAGAAAAGAGATATGACATCGACAATAATTGGAAAAT
AATCCTACAAATTTTCATTATTGCACGAGGATTTCAATTAAATCGATCAATTTCTCTTCA
ATCTTAATTTAATATTACCCCAAATGTTTTGTGAACGAAATAAAATTCGTATCTACCTTT
AGCTAATTAGTTATGATTTTAAGTTAAGGAAAGATTTTTACTAAAATTTTCTAAGAAAAT
ATTTGAAAGAAAAATTGTAAAGAATGTGGCTAGAGATAGATGTGTTGAATATATAAATAA
TAATAAATACGAAAAATGAGAATTATAGAAAAATTTGAAATGAAA

>g10883.t16 Gene=g10883 Length=622
MLSATKHLLKNTNITQTLMNTRNLSSLMKNNFSHFNRLQCRYKSEGVKGAVIGIDLGTTN
SCVAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLI
GRRFEDPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLN
QPVKNAVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVY
DLGGGTFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAM
AMQRLKEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRTI
APCQKALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSVNPDEAVAVGAAV
QGGVLAGDVTDGEREMASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVHVSARDK
GTGKEQQIVIQSSGGLSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHDTETKME
EFKDQLPKEECDKLREEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMAYKKMAS
ECESSGSKSSSEEGGEKKEDKN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
25 g10883.t16 CDD cd11733 HSPA9-like_NBD 48 425 0.0
22 g10883.t16 Coils Coil Coil 302 322 -
24 g10883.t16 Coils Coil Coil 525 545 -
23 g10883.t16 Coils Coil Coil 551 571 -
18 g10883.t16 Gene3D G3DSA:3.30.420.40 - 52 420 6.8E-161
20 g10883.t16 Gene3D G3DSA:3.30.30.30 - 114 166 6.8E-161
19 g10883.t16 Gene3D G3DSA:3.30.420.40 - 232 405 6.8E-161
17 g10883.t16 Gene3D G3DSA:3.90.640.10 Actin; Chain A 271 358 6.8E-161
16 g10883.t16 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 424 495 4.9E-27
21 g10883.t16 Gene3D G3DSA:1.20.1270.10 - 496 599 2.2E-26
29 g10883.t16 MobiDBLite mobidb-lite consensus disorder prediction 600 622 -
3 g10883.t16 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 38 430 9.6E-242
5 g10883.t16 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 38 430 9.6E-242
2 g10883.t16 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 429 580 9.6E-242
4 g10883.t16 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 429 580 9.6E-242
10 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 50 63 1.2E-55
7 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 78 90 1.2E-55
8 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 101 109 1.2E-55
12 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 186 206 1.2E-55
9 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 245 255 1.2E-55
6 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 377 393 1.2E-55
11 g10883.t16 PRINTS PR00301 70kDa heat shock protein signature 408 428 1.2E-55
1 g10883.t16 Pfam PF00012 Hsp70 protein 51 430 6.3E-190
26 g10883.t16 ProSitePatterns PS00297 Heat shock hsp70 proteins family signature 1. 54 61 -
28 g10883.t16 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 239 252 -
27 g10883.t16 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 380 394 -
14 g10883.t16 SUPERFAMILY SSF53067 Actin-like ATPase domain 51 232 1.96E-63
15 g10883.t16 SUPERFAMILY SSF53067 Actin-like ATPase domain 234 425 4.63E-66
13 g10883.t16 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 428 529 5.76E-35

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values