Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t17 isoform g10883.t17 12438172 12440188
chr_1 g10883 g10883.t17 exon g10883.t17.exon1 12438172 12438215
chr_1 g10883 g10883.t17 cds g10883.t17.CDS1 12438193 12438215
chr_1 g10883 g10883.t17 exon g10883.t17.exon2 12438279 12439528
chr_1 g10883 g10883.t17 cds g10883.t17.CDS2 12438279 12439528
chr_1 g10883 g10883.t17 exon g10883.t17.exon3 12439595 12440188
chr_1 g10883 g10883.t17 cds g10883.t17.CDS3 12439595 12439611
chr_1 g10883 g10883.t17 TTS g10883.t17 12440183 12440183
chr_1 g10883 g10883.t17 TSS g10883.t17 NA NA

Sequences

>g10883.t17 Gene=g10883 Length=1888
GCTGCTGCTTTGGCTTATGGCATGGACAAGAGTGAAGATAAAATAATTGCTGTTTATGAT
CTTGGCGGTGGAACATTCGATATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAAGTA
AAATCAACAAATGGTGATACAATGTTGGGTGGTGAGGATTTCGACAACGCAATTGTCAAT
TTCTTAGTTGATGAATTCAAGAAAGAGCAAGGTATTGATATTAGAAAGGATGCAATGGCA
ATGCAACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTGTGAATTGTCATCATCAGTTCAA
ACTGACATCAATTTGCCATATCTTACAATGGACGCAAGTGGACCTAAACACATGAACCTC
AAGATGACTCGTTCAAAATTAGAAAATCTTGTAGGAGATCTCATTAAACGTACAATTGCA
CCTTGCCAGAAAGCTTTATCCGATGCCGAAGTATCGAAATCGGATGTCGGTGAAGTTTTG
TTAGTCGGTGGTATGACAAGAATGCCCAAAGTTCAAACAACAGTTCAAGATATTTTCGGT
AGACAACCATCACGTTCAGTTAATCCAGATGAAGCTGTTGCAGTTGGTGCTGCAGTACAA
GGTGGTGTTTTGGCTGGAGATGTAACTGATGTACTTTTACTTGATGTTACACCATTGTCA
CTTGGTATTGAAACACTTGGCGGCGTCTTTACACGTTTGATCACAAGAAACACAACAATT
CCAACAAAGAAATCACAAGTATTCTCAACAGCAGCTGATGGACAAACACAAGTCGAAATC
AAAGTTCACCAGGGTGAACGTGAAATGGCATCTGATAACAAATTACTTGGAGCTTTTACA
CTTGTCGGCATTCCACCTGCTCCACGCGGTGTCCCACAAATCGAAGTTACATTTGATATT
GATGCAAACGGTATTGTACATGTATCAGCTCGTGACAAGGGAACTGGCAAGGAACAACAA
ATTGTCATTCAATCATCAGGTGGTCTCAGTAAAGATGAAATCGAAAACATGATAAAGAAT
GCCGAACAATATGCACAAGCTGATAAGACAAAGAAGGATCGAATTGAGGCTATCAATCAG
GCTGAGTCAATTGTGCACGATACAGAAACAAAAATGGAAGAATTCAAAGATCAATTACCA
AAGGAGGAATGCGATAAATTAAGAGAAGAAATTGCAAAAGTACGAACACTCCTTGCCGAT
AAAGAAAATGCAGATCCTGAAGTAGTACGTAAAGAAACTGGAGCTCTACAACAATCATCA
CTGAAACTCTTCGAAATGGCATACAAAAAAGTGAATGGCATCAGAATGTGAGAGCTCAGG
AAGCAAATCAAGTAGTGAAGAGGGTGGTGAAAAGAAGGAAGATAAAAATTAAACAAGTTC
AGACAACAACTTAAGAGGATAAATATATTTTCCCAGTAATTTTAAAAAGCATTGCATTAA
ATAAAAATCAGTTTCTGAATTAGAATTAAAACAAATACAAAAAAATAGACTTTAAACAAA
TAAAAAAACTATTAAAGGATCCAAAAAACAATTATTCAATGAATATCAATGTATTGCAAG
GATATAGAGAAAAGAGATATGACATCGACAATAATTGGAAAATAATCCTACAAATTTTCA
TTATTGCACGAGGATTTCAATTAAATCGATCAATTTCTCTTCAATCTTAATTTAATATTA
CCCCAAATGTTTTGTGAACGAAATAAAATTCGTATCTACCTTTAGCTAATTAGTTATGAT
TTTAAGTTAAGGAAAGATTTTTACTAAAATTTTCTAAGAAAATATTTGAAAGAAAAATTG
TAAAGAATGTGGCTAGAGATAGATGTGTTGAATATATAAATAATAATAAATACGAAAAAT
GAGAATTATAGAAAAATTTGAAATGAAA

>g10883.t17 Gene=g10883 Length=429
MDKSEDKIIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFK
KEQGIDIRKDAMAMQRLKEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKL
ENLVGDLIKRTIAPCQKALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSV
NPDEAVAVGAAVQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQV
FSTAADGQTQVEIKVHQGEREMASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVH
VSARDKGTGKEQQIVIQSSGGLSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHD
TETKMEEFKDQLPKEECDKLREEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMA
YKKVNGIRM

Protein features from InterProScan

Transcript Database ID Name Start End E.value
18 g10883.t17 Coils Coil Coil 74 94 -
17 g10883.t17 Coils Coil Coil 351 371 -
19 g10883.t17 Coils Coil Coil 377 397 -
14 g10883.t17 Gene3D G3DSA:3.90.640.10 Actin; Chain A 43 120 7.2E-34
15 g10883.t17 Gene3D G3DSA:3.30.420.40 - 121 177 8.0E-10
13 g10883.t17 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 193 321 8.1E-60
16 g10883.t17 Gene3D G3DSA:1.20.1270.10 - 322 425 1.2E-26
2 g10883.t17 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 6 398 2.9E-185
3 g10883.t17 PANTHER PTHR19375:SF409 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 6 398 2.9E-185
8 g10883.t17 PRINTS PR00301 70kDa heat shock protein signature 17 27 1.2E-48
5 g10883.t17 PRINTS PR00301 70kDa heat shock protein signature 149 165 1.2E-48
4 g10883.t17 PRINTS PR00301 70kDa heat shock protein signature 180 200 1.2E-48
7 g10883.t17 PRINTS PR00301 70kDa heat shock protein signature 203 222 1.2E-48
6 g10883.t17 PRINTS PR00301 70kDa heat shock protein signature 284 300 1.2E-48
1 g10883.t17 Pfam PF00012 Hsp70 protein 1 421 6.7E-174
12 g10883.t17 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 11 24 -
11 g10883.t17 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 152 166 -
10 g10883.t17 SUPERFAMILY SSF53067 Actin-like ATPase domain 6 197 2.2E-66
9 g10883.t17 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 199 355 3.92E-63

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed