General information for g10961.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as cAMP-dependent protein kinase catalytic subunit 1.
Parent gene
- The parent gene g10961 is located on chr_1: 13039070..13039810 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10961 | g10961.t1 | TTS | g10961.t1 | 13037394 | 13037394 |
| chr_1 | g10961 | g10961.t1 | isoform | g10961.t1 | 13037718 | 13039070 |
| chr_1 | g10961 | g10961.t1 | exon | g10961.t1.exon1 | 13037718 | 13038150 |
| chr_1 | g10961 | g10961.t1 | cds | g10961.t1.CDS1 | 13037718 | 13038150 |
| chr_1 | g10961 | g10961.t1 | exon | g10961.t1.exon2 | 13038442 | 13039070 |
| chr_1 | g10961 | g10961.t1 | cds | g10961.t1.CDS2 | 13038442 | 13039070 |
| chr_1 | g10961 | g10961.t1 | TSS | g10961.t1 | 13039336 | 13039336 |
Sequences
>g10961.t1 Gene=g10961 Length=1062
ATGGGAAACAACGCAACATCAGCTAATCGAAAAGTTGATGCCGAGACATCGGTCAAAGAA
TTTCTTGAACAAGCAAAAGAGGACTTTGAAGACAAGTGGAAACGAAATCCTACTAATACA
GCCTCACTTGATGACTTTGATCGTATAAAGACACTCGGTACGGGTAGTTTTGGTCGCGTA
ATGATAGTTCAACATAAACCAACTAAGGATTATTTCGCGATGAAAATATTGGATAAACAA
AAAGTGGTCAAGCTGAAGCAAGTCGAACATACACTTAATGAAAAAAGGATTTTACAAGCA
ATTTCATTTCCATTTTTGGTCAGCTTGAAGTATCACTTCAAAGATAATTCTAATTTATAT
ATGGTACTGGAGTACGTTCCAGGAGGAGAAATGTTTTCACATTTACGCAAAGTCGGACGC
TTCTCCGAACCACACTCTCGATTTTACGCTGCCCAAATTGTTCTTGCATTTGAATATCTA
CACTATTTAGATTTAATATACAGAGATTTGAAGCCAGAAAATTTATTAATCGATTCACAA
GGTTACTTGAAAGTAACCGATTTTGGATTTGCCAAGAGAGTCAAAGGTCGTACATGGACA
CTTTGTGGAACACCAGAATATCTTGCACCAGAAATAATTCTTAGCAAGGGTTATAACAAA
GCTGTGGATTGGTGGGCTCTTGGTGTTCTTGTATATGAAATGGCGGCTGGTTATCCGCCA
TTCTTCGCTGACCAACCTATTCAAATTTATGAGAAAATTGTCTCCGGAAAAGTTAGATTT
CCATCGCACTTCGGCTCAGACTTGAAAGATTTACTCAAATATTTGCTTCAAGTAGATCTT
ACAAAACGCTACGGCAACTTGAAAGCTGGTGTTAATGACATAAAGAATCACAAATGGTTC
GCATCAACCGATTGGATTGCCATTTTCCAAAAGAAGATCGAGGCTCCATTTATTCCTCGC
TGCAAAGGACCCGGTGATACAAGTAATTTCGATGATTACGAAGAGGAGGCACTTCGAATT
TCAAGCACGGAAAAATGTGCGAAAGAATTTGCAGAATTTTAA
>g10961.t1 Gene=g10961 Length=353
MGNNATSANRKVDAETSVKEFLEQAKEDFEDKWKRNPTNTASLDDFDRIKTLGTGSFGRV
MIVQHKPTKDYFAMKILDKQKVVKLKQVEHTLNEKRILQAISFPFLVSLKYHFKDNSNLY
MVLEYVPGGEMFSHLRKVGRFSEPHSRFYAAQIVLAFEYLHYLDLIYRDLKPENLLIDSQ
GYLKVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPP
FFADQPIQIYEKIVSGKVRFPSHFGSDLKDLLKYLLQVDLTKRYGNLKAGVNDIKNHKWF
ASTDWIAIFQKKIEAPFIPRCKGPGDTSNFDDYEEEALRISSTEKCAKEFAEF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10961.t1 | CDD | cd14209 | STKc_PKA | 44 | 333 | 0.0 |
| 7 | g10961.t1 | Coils | Coil | Coil | 12 | 32 | - |
| 5 | g10961.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 43 | 332 | 7.4E-130 |
| 6 | g10961.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 130 | 325 | 7.4E-130 |
| 2 | g10961.t1 | PANTHER | PTHR24353 | CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE | 10 | 353 | 5.6E-216 |
| 3 | g10961.t1 | PANTHER | PTHR24353:SF116 | CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT BETA | 10 | 353 | 5.6E-216 |
| 1 | g10961.t1 | Pfam | PF00069 | Protein kinase domain | 47 | 300 | 9.1E-70 |
| 10 | g10961.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 52 | 75 | - |
| 9 | g10961.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 165 | 177 | - |
| 14 | g10961.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 46 | 300 | 48.767 |
| 13 | g10961.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 301 | 353 | 12.503 |
| 12 | g10961.t1 | SMART | SM00220 | serkin_6 | 46 | 300 | 5.8E-104 |
| 11 | g10961.t1 | SMART | SM00133 | pkinase_C_6 | 301 | 353 | 2.8E-13 |
| 4 | g10961.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 33 | 338 | 4.72E-96 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04345 (protein kinase A)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
