General information for g10983.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable multidrug resistance-associated protein lethal(2)03659.
Parent gene
- The parent gene g10983 is located on chr_1: 13161216..13161945 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10983 | g10983.t1 | isoform | g10983.t1 | 13158614 | 13161530 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon1 | 13158614 | 13158979 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS1 | 13158614 | 13158979 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon2 | 13159035 | 13159054 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS2 | 13159035 | 13159054 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon3 | 13159122 | 13159369 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS3 | 13159122 | 13159369 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon4 | 13160537 | 13160709 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS4 | 13160537 | 13160709 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon5 | 13160773 | 13160923 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS5 | 13160773 | 13160923 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon6 | 13160983 | 13161158 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS6 | 13160983 | 13161158 |
| chr_1 | g10983 | g10983.t1 | exon | g10983.t1.exon7 | 13161216 | 13161530 |
| chr_1 | g10983 | g10983.t1 | cds | g10983.t1.CDS7 | 13161216 | 13161530 |
| chr_1 | g10983 | g10983.t1 | TTS | g10983.t1 | 13161928 | 13161928 |
| chr_1 | g10983 | g10983.t1 | TSS | g10983.t1 | NA | NA |
Sequences
>g10983.t1 Gene=g10983 Length=1449
ATGCGATTTTTCGATTTAAATCCTAGTGGAAGAATTTTGAATAGGTTCAGTAAAGATTTG
GGAGCTATTGATGAATGGCTTGCAAAATGTTTCTTAGATGCAGTTCAAGTAACATTGATG
GGTGTGGGCTCAATTATTGTCACAGCAATTATCAATCCTCTATTTCTAATACCCATTGGA
ATTTTGTTCATGATCTTTAGTTATCTGCGAAGATTCTTTTTGAAGACGTCCAAAAATCTT
AAACGTCTTGAAGGAATAACAAAATCACCTGTTTATGTACATTTAGCTGCAACAATGAAT
GGTTTGTCAACGATTAGAGCTTTTAGTGCACAGGAAGTGTTAAGAAATGAATTTGACCAC
CATCAGAATCTTCACACTGGCGCTTGGTATATGTATATAAGTGTGAGTCAGGCTTTTGGA
TTTTGTCTTGATATATTATGCTGTGCATTTGTGTGCTGCGTTACCATGAGTTTTTTGCTA
TTTGATGCAAAGTCTGCGAGCGGTTCTTCAGTTGGTTTAGCGATTACACAGTCAATGGCA
CTTACTATGATGCTGCAATGGGGAATCCGTCAAAGTGCAGAAGTAATGAACCAAATGATG
GCAGTAGAAAGAGTATTAGAATACCGTGAATTAGAACCCGAACAAAAAGAAGTCAGAAAA
GCAGTTGACAAAACGTGGCCAAAAGAAGGCGAAATTCAATTCAAAAATGTCGTTTATCGT
TATGCCAAAGAAATGGAGCCTGTATTGAGAGGTCTTAATTTTAAAATTATGCCAGGCGAG
AAAATTGGCATTGTCGGAAGAACTGGGGCTGGCAAATCATCATTAATTGGTTCGCTTTTT
AGAATAGCGGAACTCGAGGGTGAAATTTTAATAGACAACATTGATACAAGTGCCTTAAGT
TTAGAGCAGTTGCGCTCAAGAATTTCAATTATACCACAAGATCCAGTTTTATTTTCAGGA
ACGATGAGAAGAAATCTAGATCCTTTTGAAGAATATCCTGACGAATTACTTTGGAAAGCA
TTAGAAGATGTAAAATTAAAAGACATACCAGCACTTAAGATTGGCGGTTTGCAATCTTTG
GTTGCATCTGGTGGTAGCAATTTTTCCGTCGGACAACGTCAACTTATTTGTTTGGCAAGA
GCAATTTTAAGAAACAACAAAATTTTAGTTCTTGATGAGGCTACAGCAAATGTAGACCCA
GAAACGGATGGTCTAATTCAAGAAACTATTAGAAAAAAGTTTAGTGACTGTACTACATTA
ACTATAGCTCACAGACTTAATACAATTATGGATTCTGATAAAATTTTAGTTATGGATTTT
GGAAAAGTTGCAGAATTTAATACACCTTATACATTACTTCAAAACAAAAATGGAATTTTC
CGAGGAATGATTTATGCAACTGGAAATGAATCCAAAAATTTGATTGCGATGATCAAGAAA
AATGCTTAG
>g10983.t1 Gene=g10983 Length=482
MRFFDLNPSGRILNRFSKDLGAIDEWLAKCFLDAVQVTLMGVGSIIVTAIINPLFLIPIG
ILFMIFSYLRRFFLKTSKNLKRLEGITKSPVYVHLAATMNGLSTIRAFSAQEVLRNEFDH
HQNLHTGAWYMYISVSQAFGFCLDILCCAFVCCVTMSFLLFDAKSASGSSVGLAITQSMA
LTMMLQWGIRQSAEVMNQMMAVERVLEYRELEPEQKEVRKAVDKTWPKEGEIQFKNVVYR
YAKEMEPVLRGLNFKIMPGEKIGIVGRTGAGKSSLIGSLFRIAELEGEILIDNIDTSALS
LEQLRSRISIIPQDPVLFSGTMRRNLDPFEEYPDELLWKALEDVKLKDIPALKIGGLQSL
VASGGSNFSVGQRQLICLARAILRNNKILVLDEATANVDPETDGLIQETIRKKFSDCTTL
TIAHRLNTIMDSDKILVMDFGKVAEFNTPYTLLQNKNGIFRGMIYATGNESKNLIAMIKK
NA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g10983.t1 | CDD | cd18580 | ABC_6TM_ABCC_D2 | 1 | 208 | 4.72702E-86 |
| 19 | g10983.t1 | CDD | cd03244 | ABCC_MRP_domain2 | 230 | 449 | 5.79816E-137 |
| 8 | g10983.t1 | Gene3D | G3DSA:1.20.1560.10 | - | 1 | 216 | 1.1E-26 |
| 7 | g10983.t1 | Gene3D | G3DSA:3.40.50.300 | - | 217 | 466 | 3.4E-83 |
| 3 | g10983.t1 | PANTHER | PTHR24223:SF360 | MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659-LIKE PROTEIN-RELATED | 1 | 478 | 9.5E-230 |
| 4 | g10983.t1 | PANTHER | PTHR24223 | ATP-BINDING CASSETTE SUB-FAMILY C | 1 | 478 | 9.5E-230 |
| 1 | g10983.t1 | Pfam | PF00664 | ABC transporter transmembrane region | 1 | 177 | 2.0E-22 |
| 2 | g10983.t1 | Pfam | PF00005 | ABC transporter | 249 | 396 | 2.3E-30 |
| 11 | g10983.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 44 | - |
| 17 | g10983.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 45 | 69 | - |
| 10 | g10983.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 70 | 89 | - |
| 14 | g10983.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 90 | 109 | - |
| 12 | g10983.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 110 | 128 | - |
| 15 | g10983.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 129 | 159 | - |
| 9 | g10983.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 160 | 170 | - |
| 16 | g10983.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 171 | 189 | - |
| 13 | g10983.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 190 | 482 | - |
| 22 | g10983.t1 | ProSitePatterns | PS00211 | ABC transporters family signature. | 368 | 382 | - |
| 24 | g10983.t1 | ProSiteProfiles | PS50929 | ABC transporter integral membrane type-1 fused domain profile. | 1 | 197 | 23.37 |
| 25 | g10983.t1 | ProSiteProfiles | PS50893 | ATP-binding cassette, ABC transporter-type domain profile. | 232 | 465 | 18.027 |
| 23 | g10983.t1 | SMART | SM00382 | AAA_5 | 258 | 442 | 4.7E-10 |
| 5 | g10983.t1 | SUPERFAMILY | SSF90123 | ABC transporter transmembrane region | 1 | 214 | 1.24E-38 |
| 6 | g10983.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 224 | 464 | 5.69E-69 |
| 20 | g10983.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 47 | 69 | - |
| 21 | g10983.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 139 | 161 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0055085 | transmembrane transport | BP |
| GO:0005524 | ATP binding | MF |
| GO:0042626 | ATPase-coupled transmembrane transporter activity | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
