Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 4.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11137 g11137.t18 TTS g11137.t18 14302528 14302528
chr_1 g11137 g11137.t18 isoform g11137.t18 14302645 14305628
chr_1 g11137 g11137.t18 exon g11137.t18.exon1 14302645 14304120
chr_1 g11137 g11137.t18 cds g11137.t18.CDS1 14302645 14304120
chr_1 g11137 g11137.t18 exon g11137.t18.exon2 14304184 14304392
chr_1 g11137 g11137.t18 cds g11137.t18.CDS2 14304184 14304392
chr_1 g11137 g11137.t18 exon g11137.t18.exon3 14304518 14304725
chr_1 g11137 g11137.t18 cds g11137.t18.CDS3 14304518 14304725
chr_1 g11137 g11137.t18 exon g11137.t18.exon4 14305475 14305628
chr_1 g11137 g11137.t18 TSS g11137.t18 14305607 14305607

Sequences

>g11137.t18 Gene=g11137 Length=2047
CCGAAAGTTGCCGAAGATTTCAGTTTGAGTCAAAAATTCAAGTCGATAAGTTGCATTCTA
ATTAAGAGCAGATCTCAAGTCCTTAAAACTATATTTTATAAGTGATTATAAACTTACATT
TTGATAAATTAACTAATTAAAACTACAAATAAAGATGGCCGCAAAAGCTCCAGCAGTTGG
TATTGATTTGGGTACAACTTACTCGTGTGTCGGAGTTTTCCAACACGGTAAAGTTGAAAT
TATTGCCAACGATCAAGGAAACCGTACGACTCCATCGTATGTCGCATTTACTGATACTGA
AAGATTAATTGGTGATGCTGCCAAGAATCAAGTTGCAATGAATCCAACAAACACCATTTT
TGATGCTAAACGTTTGATTGGTCGCAAATTCGATGACCCAGCAGTTCAATCAGACATGAA
GCACTGGCCATTCGAAGTTGTCAGTGTTGAGGGAAAGCCTAAAATCAAGGTTACTTATAA
GGATGAAGTAAAGACTTTCTTCCCTGAAGAAATTAGTTCAATGGTTTTGGTTAAAATGAA
GGAAACTGCTGAGGCCTATTTGGGCAAGACTGATTCTGGTACCATCTCAGGATTAAATGT
TTTGCGTATTATTAACGAACCAACAGCTGCTGCTATTGCCTATGGTCTCGATAAGAAGGC
TGCAGGTGAACGCAATGTTTTGATTTTCGATTTGGGCGGTGGTACATTTGATGTTTCAAT
TCTTTCAATTGATGATGGAATTTTTGAAGTAAAGTCGACAGCCGGTGACACTCATCTTGG
TGGTGAAGATTTTGATAATCGCCTCGTTAATCATTTTGCACAAGAGTTCAAACGTAAGCA
TAAAAAGGATTTGACTTCTAACAAGCGTGCACTTCGACGTCTTAGAACAGCTTGTGAGCG
TGCAAAGAGAACTTTGTCAAGCTCGACTCAAGCCAGTATTGAAATTGATTCATTGTTCGA
GGGTATTGATTTCTACACTTCAATTACTCGTGCACGTTTCGAAGAGTTGAATGCTGATCT
CTTCCGTTCAACAATGGAGCCAGTTGAAAAAGCTATCCGTGATGCTAAAATGGACAAAGG
AACAATTCATGATATCGTTCTTGTCGGTGGCTCAACTCGTATTCCAAAAGTTCAAAAATT
ACTTCAAGACTTCTTTAACGGCAAAGAGTTGAACAAATCAATTAATCCCGATGAGGCCGT
CGCTTATGGTGCTGCTGTTCAAGCTGCCATTTTGCACGGAGACAAGAGCGAAGAAGTTCA
AGACTTGCTTCTTTTGGACGTTACTCCATTGTCACTCGGTATTGAAACAGCTGGTGGTGT
CATGAGCGTTTTGATTAAACGTAATACAACAATTCCAACCAAGCAAACTCAAACTTTCAC
CACCTATTCAGATAATCAACCAGGAGTATTGATTCAAGTATTTGAAGGCGAACGTGCTAT
GACCAAGGATAACAATCTTCTCGGTAAATTCGAACTTGCCGGTATTCCTCCTGCTCCACG
CGGTGTCCCACAAATTGAAGTTACATTTGATATCGATGCAAACGGTATTTTGAATGTTAC
TGCTTTGGAAAAATCAACAAATAAGGAAAACAAGATCACAATTACAAACGACAAGGGACG
TTTGAGCAAAGAAGATATCGAACGTATGGTTAATGAAGCTGAGAAATACCGTTCAGAGGA
TGATGCACAAAAAGATCGCATTGCAGCCAAGAACGGTTTGGAATCATACTGCTTCAACAT
GAAGGCTACAATGGAAGATGATAAATTGAAGGATAAGATCTCTGAAAGTGACAAGAAGAC
CATTTTGGACAAATGCAATGATACTATTAAATGGCTCGATGCAAATCAATTGGGTGAAAA
GGAAGAATATGAACACCGTCAAAAAGAATTAGAAGGAATTTGCAATCCAATTATCACTAA
ATTATATCAATCTGCTGGTGGTGCTCCTGGTGGTATGCCTGGCTTCCCTGGAGCTGGTGC
AGCTGGCGGTGGCGGCGCAGCAGGAGCTGGTGCAGGATCAGGTCCAACAATTGAAGAAGT
CGACTAA

>g11137.t18 Gene=g11137 Length=630
MAAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQV
AMNPTNTIFDAKRLIGRKFDDPAVQSDMKHWPFEVVSVEGKPKIKVTYKDEVKTFFPEEI
SSMVLVKMKETAEAYLGKTDSGTISGLNVLRIINEPTAAAIAYGLDKKAAGERNVLIFDL
GGGTFDVSILSIDDGIFEVKSTAGDTHLGGEDFDNRLVNHFAQEFKRKHKKDLTSNKRAL
RRLRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKA
IRDAKMDKGTIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAIL
HGDKSEEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLI
QVFEGERAMTKDNNLLGKFELAGIPPAPRGVPQIEVTFDIDANGILNVTALEKSTNKENK
ITITNDKGRLSKEDIERMVNEAEKYRSEDDAQKDRIAAKNGLESYCFNMKATMEDDKLKD
KISESDKKTILDKCNDTIKWLDANQLGEKEEYEHRQKELEGICNPIITKLYQSAGGAPGG
MPGFPGAGAAGGGGAAGAGAGSGPTIEEVD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
25 g11137.t18 CDD cd10233 HSPA1-2_6-8-like_NBD 7 361 0.0
22 g11137.t18 Gene3D G3DSA:3.30.420.40 - 9 138 6.0E-48
23 g11137.t18 Gene3D G3DSA:3.30.30.30 - 42 116 6.0E-48
20 g11137.t18 Gene3D G3DSA:3.30.420.40 - 141 355 1.8E-94
21 g11137.t18 Gene3D G3DSA:3.30.420.40 - 168 340 1.8E-94
19 g11137.t18 Gene3D G3DSA:3.90.640.10 Actin; Chain A 209 292 1.8E-94
18 g11137.t18 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 360 489 4.8E-66
24 g11137.t18 Gene3D G3DSA:1.20.1270.10 - 490 593 2.5E-39
29 g11137.t18 MobiDBLite mobidb-lite consensus disorder prediction 610 630 -
2 g11137.t18 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 138 0.0
4 g11137.t18 PANTHER PTHR19375:SF382 HEAT SHOCK-RELATED 70 KDA PROTEIN 2 1 138 0.0
3 g11137.t18 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 140 598 0.0
5 g11137.t18 PANTHER PTHR19375:SF382 HEAT SHOCK-RELATED 70 KDA PROTEIN 2 140 598 0.0
8 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 6 19 9.0E-75
9 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 34 46 9.0E-75
11 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 56 64 9.0E-75
6 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 183 193 9.0E-75
7 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 311 327 9.0E-75
10 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 343 363 9.0E-75
12 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 370 389 9.0E-75
13 g11137.t18 PRINTS PR00301 70kDa heat shock protein signature 451 467 9.0E-75
1 g11137.t18 Pfam PF00012 Hsp70 protein 7 592 4.7E-235
26 g11137.t18 ProSitePatterns PS00297 Heat shock hsp70 proteins family signature 1. 10 17 -
28 g11137.t18 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 177 190 -
27 g11137.t18 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 314 328 -
17 g11137.t18 SUPERFAMILY SSF53067 Actin-like ATPase domain 7 167 1.53E-55
16 g11137.t18 SUPERFAMILY SSF53067 Actin-like ATPase domain 171 362 3.06E-70
15 g11137.t18 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 366 523 3.53E-64
14 g11137.t18 SUPERFAMILY SSF100934 Heat shock protein 70kD (HSP70), C-terminal subdomain 518 599 2.88E-32

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values