Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 4.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11137 g11137.t20 TTS g11137.t20 14302528 14302528
chr_1 g11137 g11137.t20 isoform g11137.t20 14302645 14305628
chr_1 g11137 g11137.t20 exon g11137.t20.exon1 14302645 14304725
chr_1 g11137 g11137.t20 cds g11137.t20.CDS1 14302645 14304339
chr_1 g11137 g11137.t20 exon g11137.t20.exon2 14305475 14305628
chr_1 g11137 g11137.t20 TSS g11137.t20 14305607 14305607

Sequences

>g11137.t20 Gene=g11137 Length=2235
CCGAAAGTTGCCGAAGATTTCAGTTTGAGTCAAAAATTCAAGTCGATAAGTTGCATTCTA
ATTAAGAGCAGATCTCAAGTCCTTAAAACTATATTTTATAAGTGATTATAAACTTACATT
TTGATAAATTAACTAATTAAAACTACAAATAAAGATGGCCGCAAAAGCTCCAGCAGTTGG
TATTGATTTGGGTACAACTTACTCGTGTGTCGGAGTTTTCCAACACGGTAAAGTTGAAAT
TATTGCCAACGATCAAGGAAACCGTACGACTCCATCGTATGTCGCATTTACTGATACTGA
AAGATTAATTGGTGATGCTGCCAAGAATCAAGTTGCAATGAATCCAACAAACACCATTTT
TGGTAAGAAACATTTTTTATTTATCTATAATTCTTTGTGCTCAATGGCATTGATCTTTGC
TTAAGAGTTCATAATCTCAATTTTCTCTTTCGCTTATAATACAATATTCCTTTTTTACCA
TTTATAGATGCTAAACGTTTGATTGGTCGCAAATTCGATGACCCAGCAGTTCAATCAGAC
ATGAAGCACTGGCCATTCGAAGTTGTCAGTGTTGAGGGAAAGCCTAAAATCAAGGTTACT
TATAAGGATGAAGTAAAGACTTTCTTCCCTGAAGAAATTAGTTCAATGGTTTTGGTTAAA
ATGAAGGAAACTGCTGAGGCCTATTTGGGCAAGACTGTAACAAATGCAGTCATTACTGTT
CCAGCTTACTTCAATGACTCGCAACGTCAAGCAACAAAGGATTCTGGTACCATCTCAGGA
TTAAATGTTTTGCGTATTATTAACGAACCAACAGCTGCTGCTATTGCCTATGGTCTCGAT
AAGAAGGCTGCAGGTGAACGCAATGTTTTGATTTTCGATTTGGGCGGTGGTACATTTGAT
GTTTCAATTCTTTCAATTGATGATGGAATTTTTGAAGTAAAGTCGACAGCCGGTGACACT
CATCTTGGTGGTGAAGATTTTGATAATCGCCTCGTTAATCATTTTGCACAAGAGTTCAAA
CGTAAGCATAAAAAGGATTTGACTTCTAACAAGCGTGCACTTCGACGTCTTAGAACAGCT
TGTGAGCGTGCAAAGAGAACTTTGTCAAGCTCGACTCAAGCCAGTATTGAAATTGATTCA
TTGTTCGAGGGTATTGATTTCTACACTTCAATTACTCGTGCACGTTTCGAAGAGTTGAAT
GCTGATCTCTTCCGTTCAACAATGGAGCCAGTTGAAAAAGCTATCCGTGATGCTAAAATG
GACAAAGGAACAATTCATGATATCGTTCTTGTCGGTGGCTCAACTCGTATTCCAAAAGTT
CAAAAATTACTTCAAGACTTCTTTAACGGCAAAGAGTTGAACAAATCAATTAATCCCGAT
GAGGCCGTCGCTTATGGTGCTGCTGTTCAAGCTGCCATTTTGCACGGAGACAAGAGCGAA
GAAGTTCAAGACTTGCTTCTTTTGGACGTTACTCCATTGTCACTCGGTATTGAAACAGCT
GGTGGTGTCATGAGCGTTTTGATTAAACGTAATACAACAATTCCAACCAAGCAAACTCAA
ACTTTCACCACCTATTCAGATAATCAACCAGGAGTATTGATTCAAGTATTTGAAGGCGAA
CGTGCTATGACCAAGGATAACAATCTTCTCGGTAAATTCGAACTTGCCGGTATTCCTCCT
GCTCCACGCGGTGTCCCACAAATTGAAGTTACATTTGATATCGATGCAAACGGTATTTTG
AATGTTACTGCTTTGGAAAAATCAACAAATAAGGAAAACAAGATCACAATTACAAACGAC
AAGGGACGTTTGAGCAAAGAAGATATCGAACGTATGGTTAATGAAGCTGAGAAATACCGT
TCAGAGGATGATGCACAAAAAGATCGCATTGCAGCCAAGAACGGTTTGGAATCATACTGC
TTCAACATGAAGGCTACAATGGAAGATGATAAATTGAAGGATAAGATCTCTGAAAGTGAC
AAGAAGACCATTTTGGACAAATGCAATGATACTATTAAATGGCTCGATGCAAATCAATTG
GGTGAAAAGGAAGAATATGAACACCGTCAAAAAGAATTAGAAGGAATTTGCAATCCAATT
ATCACTAAATTATATCAATCTGCTGGTGGTGCTCCTGGTGGTATGCCTGGCTTCCCTGGA
GCTGGTGCAGCTGGCGGTGGCGGCGCAGCAGGAGCTGGTGCAGGATCAGGTCCAACAATT
GAAGAAGTCGACTAA

>g11137.t20 Gene=g11137 Length=564
MKHWPFEVVSVEGKPKIKVTYKDEVKTFFPEEISSMVLVKMKETAEAYLGKTVTNAVITV
PAYFNDSQRQATKDSGTISGLNVLRIINEPTAAAIAYGLDKKAAGERNVLIFDLGGGTFD
VSILSIDDGIFEVKSTAGDTHLGGEDFDNRLVNHFAQEFKRKHKKDLTSNKRALRRLRTA
CERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNADLFRSTMEPVEKAIRDAKM
DKGTIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILHGDKSE
EVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVFEGE
RAMTKDNNLLGKFELAGIPPAPRGVPQIEVTFDIDANGILNVTALEKSTNKENKITITND
KGRLSKEDIERMVNEAEKYRSEDDAQKDRIAAKNGLESYCFNMKATMEDDKLKDKISESD
KKTILDKCNDTIKWLDANQLGEKEEYEHRQKELEGICNPIITKLYQSAGGAPGGMPGFPG
AGAAGGGGAAGAGAGSGPTIEEVD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
20 g11137.t20 Gene3D G3DSA:3.30.420.40 - 1 137 8.0E-49
18 g11137.t20 Gene3D G3DSA:3.90.640.10 Actin; Chain A 143 215 1.4E-30
19 g11137.t20 Gene3D G3DSA:3.30.420.40 - 216 277 1.5E-16
17 g11137.t20 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 294 423 4.0E-66
21 g11137.t20 Gene3D G3DSA:1.20.1270.10 - 424 527 2.1E-39
16 g11137.t20 MobiDBLite mobidb-lite consensus disorder prediction 544 564 -
2 g11137.t20 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 532 0.0
3 g11137.t20 PANTHER PTHR19375:SF382 HEAT SHOCK-RELATED 70 KDA PROTEIN 2 1 532 0.0
8 g11137.t20 PRINTS PR00301 70kDa heat shock protein signature 56 76 2.8E-66
9 g11137.t20 PRINTS PR00301 70kDa heat shock protein signature 117 127 2.8E-66
5 g11137.t20 PRINTS PR00301 70kDa heat shock protein signature 245 261 2.8E-66
7 g11137.t20 PRINTS PR00301 70kDa heat shock protein signature 277 297 2.8E-66
4 g11137.t20 PRINTS PR00301 70kDa heat shock protein signature 304 323 2.8E-66
6 g11137.t20 PRINTS PR00301 70kDa heat shock protein signature 385 401 2.8E-66
1 g11137.t20 Pfam PF00012 Hsp70 protein 1 526 1.5E-216
15 g11137.t20 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 111 124 -
14 g11137.t20 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 248 262 -
13 g11137.t20 SUPERFAMILY SSF53067 Actin-like ATPase domain 1 101 9.71E-34
12 g11137.t20 SUPERFAMILY SSF53067 Actin-like ATPase domain 105 296 2.59E-70
11 g11137.t20 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 300 457 2.88E-64
10 g11137.t20 SUPERFAMILY SSF100934 Heat shock protein 70kD (HSP70), C-terminal subdomain 452 533 2.49E-32

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values