Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 4.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11137 g11137.t6 TTS g11137.t6 14302528 14302528
chr_1 g11137 g11137.t6 isoform g11137.t6 14302645 14303917
chr_1 g11137 g11137.t6 exon g11137.t6.exon1 14302645 14302676
chr_1 g11137 g11137.t6 cds g11137.t6.CDS1 14302645 14302676
chr_1 g11137 g11137.t6 exon g11137.t6.exon2 14302740 14303917
chr_1 g11137 g11137.t6 cds g11137.t6.CDS2 14302740 14303658
chr_1 g11137 g11137.t6 TSS g11137.t6 NA NA

Sequences

>g11137.t6 Gene=g11137 Length=1210
TCTTGGTGGTGAAGATTTTGATAATCGCCTCGTTAATCATTTTGCACAAGAGTTCAAACG
TAAGCATAAAAAGGATTTGACTTCTAACAAGCGTGCACTTCGACGTCTTAGAACAGCTTG
TGAGCGTGCAAAGAGAACTTTGTCAAGCTCGACTCAAGCCAGTATTGAAATTGATTCATT
GTTCGAGGGTATTGATTTCTACACTTCAATTACTCGTGCACGTTTCGAAGAGTTGAATGC
TGATCTCTTCCGTTCAACAATGGAGCCAGTTGAAAAAGCTATCCGTGATGCTAAAATGGA
CAAAGGAACAATTCATGATATCGTTCTTGTCGGTGGCTCAACTCGTATTCCAAAAGTTCA
AAAATTACTTCAAGACTTCTTTAACGGCAAAGAGTTGAACAAATCAATTAATCCCGATGA
GGCCGTCGCTTATGGTGCTGCTGTTCAAGCTGCCATTTTGCACGGAGACAAGAGCGAAGA
AGTTCAAGACTTGCTTCTTTTGGACGTTACTCCATTGTCACTCGGTATTGAAACAGCTGG
TGGTGTCATGAGCGTTTTGATTAAACGTAATACAACAATTCCAACCAAGCAAACTCAAAC
TTTCACCACCTATTCAGATAATCAACCAGGAGTATTGATTCAAGTATTTGAAGGCGAACG
TGCTATGACCAAGGATAACAATCTTCTCGGTAAATTCGAACTTGCCGGTATTCCTCCTGC
TCCACGCGGTGTCCCACAAATTGAAGTTACATTTGATATCGATGCAAACGGTATTTTGAA
TGTTACTGCTTTGGAAAAATCAACAAATAAGGAAAACAAGATCACAATTACAAACGACAA
GGGACGTTTGAGCAAAGAAGATATCGAACGTATGGTTAATGAAGCTGAGAAATACCGTTC
AGAGGATGATGCACAAAAAGATCGCATTGCAGCCAAGAACGGTTTGGAATCATACTGCTT
CAACATGAAGGCTACAATGGAAGATGATAAATTGAAGGATAAGATCTCTGAAAGTGACAA
GAAGACCATTTTGGACAAATGCAATGATACTATTAAATGGCTCGATGCAAATCAATTGGG
TGAAAAGGAAGAATATGAACACCGTCAAAAAGAATTAGAAGGAATTTGCAATCCAATTAT
CACTAAATTATATCAATCTGCTGGTGGTGCTCCTGGTGGATCAGGTCCAACAATTGAAGA
AGTCGACTAA

>g11137.t6 Gene=g11137 Length=316
MEPVEKAIRDAKMDKGTIHDIVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGA
AVQAAILHGDKSEEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSD
NQPGVLIQVFEGERAMTKDNNLLGKFELAGIPPAPRGVPQIEVTFDIDANGILNVTALEK
STNKENKITITNDKGRLSKEDIERMVNEAEKYRSEDDAQKDRIAAKNGLESYCFNMKATM
EDDKLKDKISESDKKTILDKCNDTIKWLDANQLGEKEEYEHRQKELEGICNPIITKLYQS
AGGAPGGSGPTIEEVD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g11137.t6 Gene3D G3DSA:3.30.420.40 - 1 52 1.3E-25
14 g11137.t6 Gene3D G3DSA:3.30.420.40 - 53 65 1.3E-25
12 g11137.t6 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 67 196 1.3E-66
15 g11137.t6 Gene3D G3DSA:1.20.1270.10 - 197 300 7.5E-40
2 g11137.t6 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 304 4.8E-177
3 g11137.t6 PANTHER PTHR19375:SF382 HEAT SHOCK-RELATED 70 KDA PROTEIN 2 1 304 4.8E-177
4 g11137.t6 PRINTS PR00301 70kDa heat shock protein signature 18 34 9.0E-47
5 g11137.t6 PRINTS PR00301 70kDa heat shock protein signature 50 70 9.0E-47
6 g11137.t6 PRINTS PR00301 70kDa heat shock protein signature 77 96 9.0E-47
7 g11137.t6 PRINTS PR00301 70kDa heat shock protein signature 158 174 9.0E-47
1 g11137.t6 Pfam PF00012 Hsp70 protein 1 299 9.9E-107
11 g11137.t6 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 21 35 -
10 g11137.t6 SUPERFAMILY SSF53067 Actin-like ATPase domain 2 69 4.43E-21
9 g11137.t6 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 73 230 7.98E-65
8 g11137.t6 SUPERFAMILY SSF100934 Heat shock protein 70kD (HSP70), C-terminal subdomain 225 306 9.42E-33

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values