General information for g1115.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Phospholipase A2.
Parent gene
- The parent gene g1115 is located on chr_3: 8277800..8278616 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1115 | g1115.t1 | isoform | g1115.t1 | 8277800 | 8278616 |
| chr_3 | g1115 | g1115.t1 | exon | g1115.t1.exon1 | 8277800 | 8277983 |
| chr_3 | g1115 | g1115.t1 | cds | g1115.t1.CDS1 | 8277800 | 8277983 |
| chr_3 | g1115 | g1115.t1 | exon | g1115.t1.exon2 | 8278135 | 8278616 |
| chr_3 | g1115 | g1115.t1 | cds | g1115.t1.CDS2 | 8278135 | 8278616 |
| chr_3 | g1115 | g1115.t1 | TSS | g1115.t1 | NA | NA |
| chr_3 | g1115 | g1115.t1 | TTS | g1115.t1 | NA | NA |
Sequences
>g1115.t1 Gene=g1115 Length=666
ATGTTGCCAACAACTTTTCTGCTCTTTGCAATTCTTGTGCTGACTTGCCATGCAGCAAAC
GAGAGAAAGCAGTCGCTGAAAGCCGTCACAGCATTTGACGATGAAGGAAGTGCAAAACAT
TCGATTGAACGTAATCGTGCTGCACAAGAGCGAATAAATCTGAGCTTCCCTGGCACTAAA
TGGTGCGGACCGGGAAATACAGCAAGTGATTATGAAGATTTGGGAAATGACGAAGAAGTT
GATAAATGCTGCCGTGATCACGATCATTGTGACAATATTGCATCAGGCGAGGAAAAATAC
GGGTTGAAAAATACTGACTTTTTTACACGACTTCATTGTCAATGTGATAAGGAATTTAAA
CAATGCTTGAGAAGTGTCAATTCAAGACGTGGAAATTATATTGGAAATTTTTATTTCAAT
TTGAGAGATAGATGTTATAAGGAACAACATCCGATTGTTGGCTGTGATGAAATTCATACA
AAAATCTTTGTAAGACGTTGCATCAGATATATAATCGATTCAATGAAGCCGTCATCGTAT
CAATGGTTCGACCTCCCGTTCTTTGACGATGAGCACGATTTCGAATATATCCCAGAGCAC
CATGACGAGCTAGACGATTTTGTTGAACAAAATGGGAATTATTTAAAAGATGGAAATTTC
CTATAA
>g1115.t1 Gene=g1115 Length=221
MLPTTFLLFAILVLTCHAANERKQSLKAVTAFDDEGSAKHSIERNRAAQERINLSFPGTK
WCGPGNTASDYEDLGNDEEVDKCCRDHDHCDNIASGEEKYGLKNTDFFTRLHCQCDKEFK
QCLRSVNSRRGNYIGNFYFNLRDRCYKEQHPIVGCDEIHTKIFVRRCIRYIIDSMKPSSY
QWFDLPFFDDEHDFEYIPEHHDELDDFVEQNGNYLKDGNFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g1115.t1 | CDD | cd04704 | PLA2_bee_venom_like | 56 | 148 | 1.51044E-44 |
| 7 | g1115.t1 | Gene3D | G3DSA:1.20.90.10 | Phospholipase A2 | 54 | 188 | 1.3E-45 |
| 2 | g1115.t1 | PANTHER | PTHR12253:SF36 | FI07253P-RELATED | 35 | 191 | 6.0E-57 |
| 3 | g1115.t1 | PANTHER | PTHR12253 | RH14732P | 35 | 191 | 6.0E-57 |
| 1 | g1115.t1 | Pfam | PF05826 | Phospholipase A2 | 56 | 148 | 4.5E-34 |
| 9 | g1115.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 10 | g1115.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 11 | g1115.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 14 | - |
| 12 | g1115.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 8 | g1115.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 221 | - |
| 15 | g1115.t1 | ProSitePatterns | PS00118 | Phospholipase A2 histidine active site. | 83 | 90 | - |
| 4 | g1115.t1 | SUPERFAMILY | SSF48619 | Phospholipase A2, PLA2 | 56 | 187 | 7.08E-31 |
| 6 | g1115.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
| 14 | g1115.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
| 5 | g1115.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050482 | arachidonic acid secretion | BP |
| GO:0006644 | phospholipid metabolic process | BP |
| GO:0004623 | phospholipase A2 activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01047 (secretory phospholipase A2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed