General information for g11269.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin-2.
Parent gene
- The parent gene g11269 is located on chr_1: 15275568..15277541 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11269 | g11269.t20 | TSS | g11269.t20 | 15274498 | 15274498 |
| chr_1 | g11269 | g11269.t20 | isoform | g11269.t20 | 15274515 | 15277320 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon1 | 15274515 | 15274569 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS1 | 15274515 | 15274569 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon2 | 15274626 | 15274664 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS2 | 15274626 | 15274664 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon3 | 15275270 | 15275380 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS3 | 15275270 | 15275380 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon4 | 15275451 | 15275595 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS4 | 15275451 | 15275595 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon5 | 15275672 | 15275784 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS5 | 15275672 | 15275784 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon6 | 15276455 | 15276601 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS6 | 15276455 | 15276601 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon7 | 15276659 | 15276692 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS7 | 15276659 | 15276692 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon8 | 15276848 | 15276899 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS8 | 15276848 | 15276899 |
| chr_1 | g11269 | g11269.t20 | exon | g11269.t20.exon9 | 15277264 | 15277320 |
| chr_1 | g11269 | g11269.t20 | cds | g11269.t20.CDS9 | 15277264 | 15277320 |
| chr_1 | g11269 | g11269.t20 | TTS | g11269.t20 | 15277409 | 15277409 |
Sequences
>g11269.t20 Gene=g11269 Length=753
ATGATTCGTCAAGTATTTTTATTAGTGACATTGGCAGTTGCTGCCTTTGCAGGACCCCTT
TCTGATAGATTCTTGAATGGAAGAATTGTTGGTGCTCCAGCAGTTGATGGACAATTCCCA
TATCAAGTTTCTCTTCGTACATTCCCTGCACTGGGACATTTCTGTGGTGGATCAATTATT
CAAGAACATTGGGTTCTTTCAGCAGCTCATTGCACAATCAACCGCACACCTGCCAATACC
CGTGTCGTTGCTGGTACAGTTCTTCTTAATGGTGGCGCAAATCCTCAACTTCATGATGTC
GAGAGAATTGTCAATCATCCAAACTACGATTCAGCCTTAATTGCTCAAGACGTTTGCGTC
ATTCGAGTGACAAGTCCGTTCATATTCACGGCAAATGTTCGTGCTATTCCTTATGCCAGT
CACTTTACTGGTGGTGGTGTTGATGCCGTTGTTTCAGGATGGGGCGGCACAGCTGTCACT
GGTGGACCTGCACCAAACAACTTACAATGGATCAGAAAAACAACGCTTACAAATGCTGAT
TGCCGTGCTCGTATGGGTACTGCTAATGAACGATTTGTTATTGACAGTAAAATTTGCACA
TTCACACAAGCTGGACAAGGAATCTGTCAAGGAGACTCAGGCGGTCCATTAACTGCAGGT
GGATATGTCATTGGTATTGTTAGCTGGAATATTCCTGATGGATATGATCGTGTTTCATAC
TGGCACAGTTGGATTACTCAAAACATCAGTTAA
>g11269.t20 Gene=g11269 Length=250
MIRQVFLLVTLAVAAFAGPLSDRFLNGRIVGAPAVDGQFPYQVSLRTFPALGHFCGGSII
QEHWVLSAAHCTINRTPANTRVVAGTVLLNGGANPQLHDVERIVNHPNYDSALIAQDVCV
IRVTSPFIFTANVRAIPYASHFTGGGVDAVVSGWGGTAVTGGPAPNNLQWIRKTTLTNAD
CRARMGTANERFVIDSKICTFTQAGQGICQGDSGGPLTAGGYVIGIVSWNIPDGYDRVSY
WHSWITQNIS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g11269.t20 | CDD | cd00190 | Tryp_SPc | 29 | 248 | 2.2208E-61 |
| 9 | g11269.t20 | Gene3D | G3DSA:2.40.10.10 | - | 23 | 250 | 2.0E-50 |
| 2 | g11269.t20 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 1 | 249 | 2.3E-49 |
| 3 | g11269.t20 | PANTHER | PTHR24276:SF78 | AT20289P-RELATED | 1 | 249 | 2.3E-49 |
| 6 | g11269.t20 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 56 | 71 | 4.0E-12 |
| 4 | g11269.t20 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 113 | 127 | 4.0E-12 |
| 5 | g11269.t20 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 206 | 218 | 4.0E-12 |
| 1 | g11269.t20 | Pfam | PF00089 | Trypsin | 29 | 244 | 6.6E-45 |
| 11 | g11269.t20 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 12 | g11269.t20 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 13 | g11269.t20 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 13 | - |
| 14 | g11269.t20 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 17 | - |
| 10 | g11269.t20 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 250 | - |
| 17 | g11269.t20 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 66 | 71 | - |
| 18 | g11269.t20 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 207 | 218 | - |
| 19 | g11269.t20 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 29 | 250 | 26.509 |
| 16 | g11269.t20 | SMART | SM00020 | trypsin_2 | 28 | 245 | 5.8E-54 |
| 7 | g11269.t20 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 6 | 249 | 3.39E-60 |
| 8 | g11269.t20 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed