General information for g11269.t49
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin-2.
Parent gene
- The parent gene g11269 is located on chr_1: 15275568..15277541 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11269 | g11269.t49 | TSS | g11269.t49 | 15274498 | 15274498 |
| chr_1 | g11269 | g11269.t49 | isoform | g11269.t49 | 15274515 | 15277320 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon1 | 15274515 | 15274569 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS1 | 15274515 | 15274569 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon2 | 15275270 | 15275380 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS2 | 15275270 | 15275380 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon3 | 15275451 | 15275595 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS3 | 15275451 | 15275595 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon4 | 15275672 | 15275784 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS4 | 15275672 | 15275784 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon5 | 15276455 | 15276601 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS5 | 15276455 | 15276601 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon6 | 15276659 | 15276692 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS6 | 15276659 | 15276692 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon7 | 15276862 | 15276903 |
| chr_1 | g11269 | g11269.t49 | cds | g11269.t49.CDS7 | 15276862 | 15276886 |
| chr_1 | g11269 | g11269.t49 | exon | g11269.t49.exon8 | 15277264 | 15277320 |
| chr_1 | g11269 | g11269.t49 | TTS | g11269.t49 | 15277409 | 15277409 |
Sequences
>g11269.t49 Gene=g11269 Length=704
ATGATTCGTCAAGTATTTTTATTAGTGACATTGGCAGTTGCTGCCTTTGCAGGACCTCCA
GCAGTTGATGGACAATTCCCATATCAAGTTTCTCTTCGTACATTCCCTGCACTGGGACAT
TTCTGTGGTGGATCAATTATTCAAGAACATTGGGTTCTTTCAGCAGCTCATTGCACAATC
AACCGCACACCTGCCAATACCCGTGTCGTTGCTGGTACAGTTCTTCTTAATGGTGGCGCA
AATCCTCAACTTCATGATGTCGAGAGAATTGTCAATCATCCAAACTACGATTCAGCCTTA
ATTGCTCAAGACGTTTGCGTCATTCGAGTGACAAGTCCGTTCATATTCACGGCAAATGTT
CGTGCTATTCCTTATGCCAGTCACTTTACTGGTGGTGGTGTTGATGCCGTTGTTTCAGGA
TGGGGCGGCACAGCTGTCACTGGTGGACCTGCACCAAACAACTTACAATGGATCAGAAAA
ACAACGCTTACAAATGCTGATTGCCGTGCTCGTATGGGTACTGCTAATGAACGATTTGTT
ATTGACAGTAAAATTTGCACATTCACACAAGCTGGACAAGGAATCTGTCAAGGAGACTCA
GGCGGGTGGATATGTCATTGGTATTGTTAGCTGGAATATTCCTGTAAGATGGATATGATC
GTGTTTCATACTGGCACAGTTGGATTACTCAAAACATCAGTTAA
>g11269.t49 Gene=g11269 Length=209
MIRQVFLLVTLAVAAFAGPPAVDGQFPYQVSLRTFPALGHFCGGSIIQEHWVLSAAHCTI
NRTPANTRVVAGTVLLNGGANPQLHDVERIVNHPNYDSALIAQDVCVIRVTSPFIFTANV
RAIPYASHFTGGGVDAVVSGWGGTAVTGGPAPNNLQWIRKTTLTNADCRARMGTANERFV
IDSKICTFTQAGQGICQGDSGGWICHWYC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g11269.t49 | CDD | cd00190 | Tryp_SPc | 18 | 202 | 2.60029E-50 |
| 10 | g11269.t49 | Gene3D | G3DSA:2.40.10.10 | - | 18 | 202 | 6.3E-43 |
| 9 | g11269.t49 | Gene3D | G3DSA:2.40.10.10 | - | 27 | 122 | 6.3E-43 |
| 2 | g11269.t49 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 18 | 202 | 4.4E-37 |
| 3 | g11269.t49 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 43 | 58 | 5.5E-10 |
| 5 | g11269.t49 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 100 | 114 | 5.5E-10 |
| 4 | g11269.t49 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 193 | 205 | 5.5E-10 |
| 1 | g11269.t49 | Pfam | PF00089 | Trypsin | 18 | 202 | 5.6E-38 |
| 12 | g11269.t49 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 24 | - |
| 13 | g11269.t49 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 14 | g11269.t49 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 15 | g11269.t49 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 24 | - |
| 11 | g11269.t49 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 25 | 209 | - |
| 20 | g11269.t49 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 53 | 58 | - |
| 21 | g11269.t49 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 15 | 209 | 20.539 |
| 19 | g11269.t49 | SMART | SM00020 | trypsin_2 | 14 | 204 | 8.2E-29 |
| 6 | g11269.t49 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 8 | 202 | 3.03E-47 |
| 8 | g11269.t49 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 17 | g11269.t49 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 7 | g11269.t49 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 17 | - |
| 18 | g11269.t49 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed