General information for g11339.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Insulin-degrading enzyme.
Parent gene
- The parent gene g11339 is located on chr_1: 15727486..15735873 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11339 | g11339.t3 | TSS | g11339.t3 | 15723555 | 15723555 |
| chr_1 | g11339 | g11339.t3 | isoform | g11339.t3 | 15723622 | 15725384 |
| chr_1 | g11339 | g11339.t3 | exon | g11339.t3.exon1 | 15723622 | 15723674 |
| chr_1 | g11339 | g11339.t3 | cds | g11339.t3.CDS1 | 15723622 | 15723674 |
| chr_1 | g11339 | g11339.t3 | exon | g11339.t3.exon2 | 15723838 | 15724822 |
| chr_1 | g11339 | g11339.t3 | cds | g11339.t3.CDS2 | 15723838 | 15724822 |
| chr_1 | g11339 | g11339.t3 | exon | g11339.t3.exon3 | 15724893 | 15725384 |
| chr_1 | g11339 | g11339.t3 | cds | g11339.t3.CDS3 | 15724893 | 15725384 |
| chr_1 | g11339 | g11339.t3 | TTS | g11339.t3 | NA | NA |
Sequences
>g11339.t3 Gene=g11339 Length=1530
ATGTATAGTGTTCGGATTATTTCCTGTGCGCTTGTAGAGAGACATATTGTGAAAAAGAAA
AACTGGTTTGGATTAAGTAGGAGATTCTTATCATCATCTACATTATCAAATAATACTCTT
GTAATGAAAACAGCAGAAAAAGGAAAATCAAATATCTCTGGATCTATTTTATCAAGCGTA
CAAAATATGGAAATAATCAGATATGAGAATATTGTAAAAAGTGAAGAGGACAAGCGTTTG
TATAGAGGACTTAAACTAGAAAATGGTATCAAAGTGCTCCTTATATCTGATCCTGAAACT
GATAAAAGTGCTGCATGCATGTCGATTGAAATCGGGCATATGTCTGATCCAATATCATTA
CCGGGCTTAGCTCATTTCGTAGAACATATGCTCTTTTTAGGAACTAAAAAATATCCAAAT
GAAAATGAATATAGCAGTTTTTTGAGCAAAAATAGTGGCACTTCAAATGCTGCAACGTAT
CCAGATTTGACTAAATATTACTTTGATGTCATGCCTGAGAAACTTGAGGGCGCCATTGAT
CGATTTTCTCAATTTTTTACAAGTCCTTTATTCACTGAAAGTGCAACATTGAGAGAAATT
AATGCAGTTCATTCTGAGCACGAAAAAAACTTGGCAACAGATGTATGGCGAATTAGACAA
GTTCAAAAATCACTTGCTAATAAAAATCATCCTTATTCAAAATTCGGCACTGGTAATAAA
GATACACTTTTAGATATTCCAAAGAAAAATGGCGTGGACACTCGTACAGAACTTATTAAA
TTTCATGACAAATACTATTCATCAAACCTCATGACACTTGCAATTTTTGGAAAAGAATCT
CTTGATGAACTTGAAGTTTTAGCAACGAAATATTTCTCTAATATAGAGAATAAAAATGTT
CCACTTCCAAAATGGGGTGATGATGTCTATTTAGACGATCAAATAAGAACAAAACTTTAT
ATTGTACCTGTAAAAGATACAAGAACTTTGTCGATGTCTTTTCAAATTCCTGATATGGAC
GAATATTTTAGAAGTGGACCTGAACATTACATTTCTCATTTAGTAGGTCATGAAGGTAAA
GGTTCAATTTTATCCGAATTAAAGAAACGTGGATGGTGCAATAATTTGATAGGAGGAGCT
AGTTCCTCTGCAAAAGGTTTTGGGTTCTTTGAAATTACTGTAGATTTGACAGAGGAAGGA
TTTGAACATATTGACGACATTTGCAAACTTATATTTCAATATCTAAATTTATTAAGAAAA
GAGGGTCCACAACAATGGATTTTTGATGAATATCGAAAACTTAGTGAAATGCAATTTCGC
TTCAAAGATAAAGAAAACCCAATAAACCTTGTATCTAACATTGTTCACCAAATGATGATT
TATCCCCTTTCTGATGTTCTTTCCGCTAACTATCTTCTTACTGAATGGCGGCCTGATTTA
ATTAATGATGTCCTGACACGTTTGAATCCACAAAATTGTCGAATAACTATAGTCGGTCAA
AAAGTTAGTGATAAATGTAAAGAGGAAGAA
>g11339.t3 Gene=g11339 Length=510
MYSVRIISCALVERHIVKKKNWFGLSRRFLSSSTLSNNTLVMKTAEKGKSNISGSILSSV
QNMEIIRYENIVKSEEDKRLYRGLKLENGIKVLLISDPETDKSAACMSIEIGHMSDPISL
PGLAHFVEHMLFLGTKKYPNENEYSSFLSKNSGTSNAATYPDLTKYYFDVMPEKLEGAID
RFSQFFTSPLFTESATLREINAVHSEHEKNLATDVWRIRQVQKSLANKNHPYSKFGTGNK
DTLLDIPKKNGVDTRTELIKFHDKYYSSNLMTLAIFGKESLDELEVLATKYFSNIENKNV
PLPKWGDDVYLDDQIRTKLYIVPVKDTRTLSMSFQIPDMDEYFRSGPEHYISHLVGHEGK
GSILSELKKRGWCNNLIGGASSSAKGFGFFEITVDLTEEGFEHIDDICKLIFQYLNLLRK
EGPQQWIFDEYRKLSEMQFRFKDKENPINLVSNIVHQMMIYPLSDVLSANYLLTEWRPDL
INDVLTRLNPQNCRITIVGQKVSDKCKEEE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g11339.t3 | Gene3D | G3DSA:3.30.830.10 | Cytochrome Bc1 Complex; Chain A | 54 | 302 | 1.2E-93 |
| 10 | g11339.t3 | Gene3D | G3DSA:3.30.830.10 | Cytochrome Bc1 Complex; Chain A | 303 | 510 | 2.0E-80 |
| 4 | g11339.t3 | PANTHER | PTHR43690:SF18 | INSULIN-DEGRADING ENZYME-RELATED | 68 | 504 | 4.9E-192 |
| 5 | g11339.t3 | PANTHER | PTHR43690 | NARDILYSIN | 68 | 504 | 4.9E-192 |
| 1 | g11339.t3 | Pfam | PF00675 | Insulinase (Peptidase family M16) | 91 | 227 | 3.5E-37 |
| 3 | g11339.t3 | Pfam | PF05193 | Peptidase M16 inactive domain | 254 | 433 | 7.3E-25 |
| 2 | g11339.t3 | Pfam | PF16187 | Middle or third domain of peptidase_M16 | 439 | 509 | 1.3E-17 |
| 8 | g11339.t3 | ProSitePatterns | PS00143 | Insulinase family, zinc-binding region signature. | 112 | 135 | - |
| 6 | g11339.t3 | SUPERFAMILY | SSF63411 | LuxS/MPP-like metallohydrolase | 70 | 303 | 2.44E-60 |
| 7 | g11339.t3 | SUPERFAMILY | SSF63411 | LuxS/MPP-like metallohydrolase | 306 | 508 | 2.17E-70 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046872 | metal ion binding | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
