Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative fatty acyl-CoA reductase CG8306.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11424 g11424.t24 TSS g11424.t24 16153130 16153130
chr_1 g11424 g11424.t24 isoform g11424.t24 16153892 16155422
chr_1 g11424 g11424.t24 exon g11424.t24.exon1 16153892 16154479
chr_1 g11424 g11424.t24 cds g11424.t24.CDS1 16153900 16154479
chr_1 g11424 g11424.t24 exon g11424.t24.exon2 16154537 16154963
chr_1 g11424 g11424.t24 cds g11424.t24.CDS2 16154537 16154963
chr_1 g11424 g11424.t24 exon g11424.t24.exon3 16155026 16155422
chr_1 g11424 g11424.t24 cds g11424.t24.CDS3 16155026 16155422
chr_1 g11424 g11424.t24 TTS g11424.t24 16155626 16155626

Sequences

>g11424.t24 Gene=g11424 Length=1412
AGATTTACATGCTGATTCGCTCAAAGAAGGGCAAATCTATTCAAGAACGTTTACAAGACA
TTACAAAAAATTCTGTTTTCTCATTGTTACTTGAAGAACGGTCGCAAGATGTATTTAATA
AATTGATTCCTATTGAAGGAGATGTTGGCGAAGAAAACCTTGCATTATCAGCCAGTGACC
GTCAGTTGCTAATAGATAACGTCAATATTGTTATCCATTCTGCAGCAACTCTTGATTTTC
AGGCATCATTAAAACCAACTGTTTTAATTAATCTTTTGGGAACTCGACAAATAATGCAGT
TATGCTCTCAAATGAAAAGTTTGGTGGCAATGACACATGTTTCGAGTGCTTATGTTAATT
CATTTTTGCTTGAAACTGAAGAAATACTGTATCCAGCTCCAGAAGATCCTGAAAAGGTCA
TTGAGCTTGTTAAAAATAAAACGGAAAGCGAGTTGGAAACTCTTACTCCTGGTTTGATAA
AGTCGCATCCAAATACATATACCTTTACGAAGCATTTAGCCGAGCATGAAGTTAATAAAT
ATGCGCAAAAATTTCCTTGCGCAATTGTTCGACCATCTATGATTACAGCGAGTTGGATGG
AACCTAGAAAAGGTTGGACAATTAGTAAAAATGGTCCACAAGGATTTTTGATGGGATCAT
CTAAAGGTGTTATTCGTCGTCTTCCCGTCAATACAAAGCTAATTTACGATTACATCCCTG
TTGACACTGTTGTCAATGAAATTTTGGTTGCTGCTTATCATGTCGCTCAAAAGAAATCTG
GAGAAGTTTCGATTTTCCACTGCACTACTTCCACCTACAATCCTTTCAAATGGGACAAAG
TTGCTGATAAAACTAACGAGCTTTTACACATGTATCCACTTAAATCAGCCGTTTGGTATC
CGCATTTAAAATTTGTTTCAAGCTTGATGCTTTTTAAAATTTCTGCAATTTTTGTTCACT
TTTTACCAGCATACATTCTTGATTTCATTACACGTTTATCTGGAGGCCGACCAATTTTGG
TTCGCCTGCATACCAATGTTTGGGAATCTTTGAAACTTCTTGAGAAATTCATCTTTACTG
AATGGAAATTTCATAACAAAAACACTTTAATGCTACACAAAACTATGTCTCCGACAGATC
AGCAAACATTCAACATTGATGTCGGCAAGCTCGAATGGGAAGATTATTTCATTAATCTTG
CTCAAGGTGTACGTCAGTATCTAAATAACGAAACATTAAAAACACTCCCAGCTGCAAAAA
AGAAGGATAAAATTTTGTTGATTTTACACATTTTTTTACAAGTTGGAATTCATTGTGCTA
TGTGGAAATTATTTTCATGCATTTTTGGTGTCTCAATGATGAAATGTATTTTTGCACTTC
CAATTTCTTATTTCGTACTAAACATATTATAA

>g11424.t24 Gene=g11424 Length=467
MLIRSKKGKSIQERLQDITKNSVFSLLLEERSQDVFNKLIPIEGDVGEENLALSASDRQL
LIDNVNIVIHSAATLDFQASLKPTVLINLLGTRQIMQLCSQMKSLVAMTHVSSAYVNSFL
LETEEILYPAPEDPEKVIELVKNKTESELETLTPGLIKSHPNTYTFTKHLAEHEVNKYAQ
KFPCAIVRPSMITASWMEPRKGWTISKNGPQGFLMGSSKGVIRRLPVNTKLIYDYIPVDT
VVNEILVAAYHVAQKKSGEVSIFHCTTSTYNPFKWDKVADKTNELLHMYPLKSAVWYPHL
KFVSSLMLFKISAIFVHFLPAYILDFITRLSGGRPILVRLHTNVWESLKLLEKFIFTEWK
FHNKNTLMLHKTMSPTDQQTFNIDVGKLEWEDYFINLAQGVRQYLNNETLKTLPAAKKKD
KILLILHIFLQVGIHCAMWKLFSCIFGVSMMKCIFALPISYFVLNIL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
17 g11424.t24 CDD cd05236 FAR-N_SDR_e 2 290 2.52937E-111
18 g11424.t24 CDD cd09071 FAR_C 315 405 3.38282E-33
9 g11424.t24 Gene3D G3DSA:3.40.50.720 - 1 308 4.9E-58
3 g11424.t24 PANTHER PTHR11011:SF27 FATTY ACYL-COA REDUCTASE CG8306-RELATED 1 451 3.8E-221
4 g11424.t24 PANTHER PTHR11011 MALE STERILITY PROTEIN 2-RELATED 1 451 3.8E-221
1 g11424.t24 Pfam PF07993 Male sterility protein 1 244 9.5E-57
2 g11424.t24 Pfam PF03015 Male sterility protein 317 408 1.2E-29
13 g11424.t24 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 301 -
15 g11424.t24 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 302 324 -
11 g11424.t24 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 325 421 -
16 g11424.t24 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 422 442 -
12 g11424.t24 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 443 447 -
14 g11424.t24 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 448 466 -
10 g11424.t24 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 467 467 -
8 g11424.t24 SUPERFAMILY SSF51735 NAD(P)-binding Rossmann-fold domains 4 293 1.32E-19
5 g11424.t24 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 302 324 -
7 g11424.t24 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 422 439 -
6 g11424.t24 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 444 466 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0080019 fatty-acyl-CoA reductase (alcohol-forming) activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values