General information for g11515.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Mitotic checkpoint serine/threonine-protein kinase BUB1.
Parent gene
- The parent gene g11515 is located on chr_1: 16751264..16752191 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11515 | g11515.t1 | TSS | g11515.t1 | 16745664 | 16745664 |
| chr_1 | g11515 | g11515.t1 | isoform | g11515.t1 | 16746271 | 16751264 |
| chr_1 | g11515 | g11515.t1 | exon | g11515.t1.exon1 | 16746271 | 16746383 |
| chr_1 | g11515 | g11515.t1 | cds | g11515.t1.CDS1 | 16746271 | 16746383 |
| chr_1 | g11515 | g11515.t1 | exon | g11515.t1.exon2 | 16746444 | 16746648 |
| chr_1 | g11515 | g11515.t1 | cds | g11515.t1.CDS2 | 16746444 | 16746648 |
| chr_1 | g11515 | g11515.t1 | exon | g11515.t1.exon3 | 16746721 | 16749840 |
| chr_1 | g11515 | g11515.t1 | cds | g11515.t1.CDS3 | 16746721 | 16749840 |
| chr_1 | g11515 | g11515.t1 | exon | g11515.t1.exon4 | 16749893 | 16750764 |
| chr_1 | g11515 | g11515.t1 | cds | g11515.t1.CDS4 | 16749893 | 16750764 |
| chr_1 | g11515 | g11515.t1 | exon | g11515.t1.exon5 | 16750823 | 16751264 |
| chr_1 | g11515 | g11515.t1 | cds | g11515.t1.CDS5 | 16750823 | 16751264 |
| chr_1 | g11515 | g11515.t1 | TTS | g11515.t1 | 16751367 | 16751367 |
Sequences
>g11515.t1 Gene=g11515 Length=4752
ATGGATTTAGATACTGCAAAAGAGAACATTTTACCATTAAAAAGTGGTCGTCGTGCAGAT
TTAATAGAATCAGCTTTGGGTAATGAAAAAGAGATGCTTGAATTAGAGAAACAACGATTT
GAAGAAGAAATAGCACATTATGATGGAAAAGATCCTTTAGATCCATGGTATAAGTATATT
TGCTGGATTGAACAAACTCATTTCAAAAGTGGCAATGATGGAGGCTTGCAAGAAGTAATT
CAAAAATGTATCGTCAAATTTGAAAACGAAAAAAGATATAAGCAAGATAGACGGTTAATA
AAACTTTTTATAAAATATATTGATACGAGAAGCAATCCCCAAGAGTTGTATCTTCAACTT
TATAATAATGGCATTGGAACATTGGTTGCTGATTTATACATCGCATGGAGTTATTATTTT
GATGTTATAAATGATTTTCAAAACGCCGATTCTATTTTTCAAAAAGGAATCGATGCAGGT
GCTCAACCATATGATGAATTAATTCAAGCGCATCAAGCTTTCAATGTTTCGATAACAAAA
AGAATTCTTTGCAAAGATGACAACATAAAAAAACAATTTCAAGAAACAATGGACGAAAGA
AGACATGCACTAACAAGATTGAATGCTCATAAAAATAAATATGTGGGAAGTATTAGAACT
GGCATTGCTGTAAAGCATGAAAATCCAGGAAAATTTATAGTAGATGAAAATCAAAGTTCA
TCAAATCATCAAAAAATAGTTGTTCATCAAGATCTAGAAGTAGCTTCAAATTTTGATTCA
ATGTCGCTTGTAGGTAAGAGTATTCTAGAAACAGCTAAGACAAACGAAAACATCCATGAG
CCTGGTCCTTGGACTAAAGCAAAAGTAAAGGTCAAAGGAAAGGGTGTTTTTACAACTGCA
CTTATAGGAGCTACATCGTCGAATTTCAATATTTTGGAAGATGATACTATTTATGAACCG
ATCTTATGTCCAAGAAAACTATATGAGAAAGGTATTTGTTTGCCACCTGGTTTTATTTCC
AAAAATAAACCACAAAAGTCTTGGGAAGTACCAATTGTTATTGAAGAATCACAAATACCA
AATTCTATTCCTAAATATGACAAATTTTTGACTTATCCAGCTAGAGGAGTTGAAATATCT
CTCGAAGAAATTCGAGGATATAAATGGTTCAAAAATCAAAATAAAACTTCATCTCTTACT
GTTCAATACGATTTTATTTTGACCAACAAATTTGAAAATGGATGTCGCATTATTCCTGGT
TTTACAAACAAAAACATTGAACAAAGTAAAATTAACTATCCTTTGACAAATGAAGTAACA
GATGGTAAAATTATGTGTAAAATAAATGAAATGATCACAGAAAGTAGTGAAATATCAACA
GAAGAATTGCTTGCTAGAAAATTCTATAGAGGAGAGATAAAATTAGTTACTGCTGATGAT
TTTGAGGAGATTGAAAATAATGATGACATGGACATTACTCAAATCGGAGATCGGCGTCAA
TCTTTTTATCCTCTTTCAAGAATGTCGATAATTCCAAGAAAATCTATATTAAGAAAATCT
GTTTTCCAACCGGCAATTGTAGAAAATCCAGAAGATGACACAACTAAAAATGATTATCGT
CATCGTGTACGATTTGAAAATCCTTTACAAGAGATGCGAGAAGAGGGAGAAAATGAAAAA
GCTGATGACTCAAACAATTCAATTACTAAAAAGCGTAAACTTAATGAAGATGTTGATCAG
GTTAATATTTCTGAGAAAAATTTAAATTCAGAAGATAAAAGCGATGGAGAAGAAACTCTT
AAAGCACAAATTCATCATTCTTCAGTTTTGAATACAATAAAACCTGAATTTATTAATGAT
AAAAATGAAAATTTTCCAAAGATTGATAGTAAATTGGAATTGTTTAAACTACCACCACCT
ATTGAGAAAAAAATTTTCAAACCATTTGATTTAACCGAAGACGATGCAAATGATACATGC
TCAACAGTACAATTTAATTTATTTATAAAGCAACAGTCTGTTAGTACTCCAATATCAAAG
AAATCTGTTCCAAAATTAGTTCCACTAGCTAATCAAGAATCTTCTACTTCACAAGCTAAT
GCTTTTACAATTTACACGGATGATCATAAAAACGAAGATGTTAGTGATAAAAATGAAATT
CAAGAGCAAGGTTCAGAAAATACCGGCGTATGTGCGTCAATAATGCAAACAAATGTATTA
CAACCAAAACTTTCAACAATTATGGAAGTTACTGAAACAACACAATCTACAAAATCAAAT
GTAAGCTCGATTAATGATGCTGAAAGTCAAGCTTTAAAATCACAATTAAAGAACACTCCG
CCTGAATCTGACAAACATTTTAATAATTTGAGTGTATTTCGTATACCTGAAGATCAAACA
GAAACAATTCCGTTTAATATATTAAAATTAGCACAAATTCCAAAAACACCTGGACGTTTT
CTATGTTTGGAAGAAAGTGATGTGAAACAAAAGCAGCAATTAAATGATGATAAACAACAA
CAATCATTTATGACAATACCAAAATTTGAATTCTCTTTCACAGGTGGTGGTGATATGTCA
ATCAATAATTTTTCTATTATGAAACCATCTCTAATCACACAAGAGAAAAAAGAACTTTCT
TATGTGGAAATCCCAGCAACGCAAGAATGCACTATTGAAAACGAAAAATCACATCTAACA
GCCCCGATAACACAAGAAATTTTAGAAAAGAAACAAAACTTGGAAATAGAAACAGCTACT
CCTCAACCAATATCAGAAAAAAACACAATTGAAAATGTTGAACAAAATAATGTTAAAGAA
CTAACCATTTCACATGTTGAGATATCTGCAACACAAACTGAATTATTGCATACTGAGCTC
CCAAACGCCCAAGATTCGAATAAATTGGTTGAGGAACAACAAGGTTTCAAATTTGATATT
TATGAAGATACAACAGCTTGTAATGCTATTCCGCTCGTTCGTATTCAACCGCAAAATATT
AAACTTGATGAAACAAATAATTTTCATGCCTCGAGAAAAGAAAATTATGTCGATAGTATG
CTCAAACGAAATGTTTCTGATGAATTTTTAAACCTCTGCTCAAAATCTCCAGCTTTAACT
AAAACAATTAATTGCCCAGATAAAGAAGTTAGTGATCTGCTCAAGTTTTCTGACATAAAA
ACAAATAAGACTCTTGAAAATAGTTTTCAAACACTTGCTATAGAACATGCTGTTAAAAGT
GCATCACCCACAATGAACATATTTGAAGAAGATCTTAACACACAGAAATTTAGCTCACTC
TTGACAACTGATAAGAATTCAACTATCATAGATTCGATTTCTCAAAAGCAGCAAATTAGT
GAATCAAAAAGTGCAAAAAAACCTCTGGACATTAGCATTGAAATGGACGAAACAGAAACT
TCACGACTTAATATAGAGAAATCTGATGCAGTTTTTAAAGTTCCACAAATTCCAATAAGC
AAACCAACAGTGGTACAAAATAATCAAAACAAATTTGAAATATTTGAGGATGAAGAAGAA
GAGGAAGATGATGATATGAGTAAAACGATCTATTTCGATGATGATGAAAAGAAAAGAGAG
GAAATCGTTGATGATGAGATAAATTCTTGGGAAGAAGAAGAGATGTGTAGTTTTATTTTA
AATTCTGAAAATCGCTATGAACATACAATGATTCAAAGCGATGTATCAAAGAAACTGCGC
GAAGCAATTATTATTTCAAATGGTAATCCTTTCTGTAAAAATGTTCGAAATTTAATACTG
GAACATGTTAATTTTGAAGAATACTTAGCTGAACATGTTCAAAATTGTATAATGCTAAAA
AATATTCCGCAACTAAAAATTGGCGTTTCATTGGAATGTGGCAAAGATAATTTCAAAGTA
ATGAAGTTCATTGCAAAGGGAACGTTTGGATCCGTCTATTGTATAAAAAATCAGAAAAGT
GGTGATCTTTTTGCTGCCAAACAAGAAAAGCCCGTAAATATGTATGAATATTTTATTTGT
CTCGAGTTAGCAGATCGATTAAGAGGAAAAAATATTCATATGCTTAAAGCATTTATGCAA
ATTCACTATGCAATCATTGCGAATAATGCAAGCGTCTTCATAACTGATCTTTCTCCTTAT
GGAACACTTATTGATGTTTGCAACAAAATTAAACATGTCACTGGTCGTAATGTTGATGAA
TACATTGGTATGATTTTCACTTCGCAACTGTTGTCCATCATTGATTTCCTACATTCATGT
CATATCATTCATGCTGATTTAAAACCTGATAATTTACTCTTAATGTCAAAAGTGGAAATT
GGTATGAAGGTGCCAGCTCTACAACTTATTGATTTTGGGTCTGCTATAGATATGGAGAAT
TACTCAAAAGATGACACATTCAATTATGTGGTTGAAACAGAAAATTTCACCTGTTGCGAA
ATGAAGGAAAACAGACCATGGACGTATCAAACTGATTTATTTGGAATTTGTGGTACAGCT
CATGTAATTTTATTTGGCAAATATATGACAGTTGAAAAAAAATTATTAAATTGGGAGCCA
ACGACAAAATTCCCTCGATATTTTCAAAAATCTTTATGGGATATATTTTTCAATACTTTA
TTAAACGTTCCTGATTGTAACTTTATGCCGAATCTTCAACATTTAAAGCAAAATTTTGAT
GTGGAGATTATGGAAAGACAAAAATATGTGAATGAAAAAATTGCAGTTTTTAATAATGCT
TTAATGAGTTAA
>g11515.t1 Gene=g11515 Length=1583
MDLDTAKENILPLKSGRRADLIESALGNEKEMLELEKQRFEEEIAHYDGKDPLDPWYKYI
CWIEQTHFKSGNDGGLQEVIQKCIVKFENEKRYKQDRRLIKLFIKYIDTRSNPQELYLQL
YNNGIGTLVADLYIAWSYYFDVINDFQNADSIFQKGIDAGAQPYDELIQAHQAFNVSITK
RILCKDDNIKKQFQETMDERRHALTRLNAHKNKYVGSIRTGIAVKHENPGKFIVDENQSS
SNHQKIVVHQDLEVASNFDSMSLVGKSILETAKTNENIHEPGPWTKAKVKVKGKGVFTTA
LIGATSSNFNILEDDTIYEPILCPRKLYEKGICLPPGFISKNKPQKSWEVPIVIEESQIP
NSIPKYDKFLTYPARGVEISLEEIRGYKWFKNQNKTSSLTVQYDFILTNKFENGCRIIPG
FTNKNIEQSKINYPLTNEVTDGKIMCKINEMITESSEISTEELLARKFYRGEIKLVTADD
FEEIENNDDMDITQIGDRRQSFYPLSRMSIIPRKSILRKSVFQPAIVENPEDDTTKNDYR
HRVRFENPLQEMREEGENEKADDSNNSITKKRKLNEDVDQVNISEKNLNSEDKSDGEETL
KAQIHHSSVLNTIKPEFINDKNENFPKIDSKLELFKLPPPIEKKIFKPFDLTEDDANDTC
STVQFNLFIKQQSVSTPISKKSVPKLVPLANQESSTSQANAFTIYTDDHKNEDVSDKNEI
QEQGSENTGVCASIMQTNVLQPKLSTIMEVTETTQSTKSNVSSINDAESQALKSQLKNTP
PESDKHFNNLSVFRIPEDQTETIPFNILKLAQIPKTPGRFLCLEESDVKQKQQLNDDKQQ
QSFMTIPKFEFSFTGGGDMSINNFSIMKPSLITQEKKELSYVEIPATQECTIENEKSHLT
APITQEILEKKQNLEIETATPQPISEKNTIENVEQNNVKELTISHVEISATQTELLHTEL
PNAQDSNKLVEEQQGFKFDIYEDTTACNAIPLVRIQPQNIKLDETNNFHASRKENYVDSM
LKRNVSDEFLNLCSKSPALTKTINCPDKEVSDLLKFSDIKTNKTLENSFQTLAIEHAVKS
ASPTMNIFEEDLNTQKFSSLLTTDKNSTIIDSISQKQQISESKSAKKPLDISIEMDETET
SRLNIEKSDAVFKVPQIPISKPTVVQNNQNKFEIFEDEEEEEDDDMSKTIYFDDDEKKRE
EIVDDEINSWEEEEMCSFILNSENRYEHTMIQSDVSKKLREAIIISNGNPFCKNVRNLIL
EHVNFEEYLAEHVQNCIMLKNIPQLKIGVSLECGKDNFKVMKFIAKGTFGSVYCIKNQKS
GDLFAAKQEKPVNMYEYFICLELADRLRGKNIHMLKAFMQIHYAIIANNASVFITDLSPY
GTLIDVCNKIKHVTGRNVDEYIGMIFTSQLLSIIDFLHSCHIIHADLKPDNLLLMSKVEI
GMKVPALQLIDFGSAIDMENYSKDDTFNYVVETENFTCCEMKENRPWTYQTDLFGICGTA
HVILFGKYMTVEKKLLNWEPTTKFPRYFQKSLWDIFFNTLLNVPDCNFMPNLQHLKQNFD
VEIMERQKYVNEKIAVFNNALMS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g11515.t1 | Coils | Coil | Coil | 23 | 50 | - |
| 11 | g11515.t1 | Coils | Coil | Coil | 571 | 591 | - |
| 8 | g11515.t1 | Gene3D | G3DSA:1.25.40.430 | - | 1 | 177 | 1.0E-47 |
| 9 | g11515.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1296 | 1582 | 6.9E-84 |
| 15 | g11515.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 546 | 596 | - |
| 3 | g11515.t1 | PANTHER | PTHR14030:SF4 | BUB1 KINASE, ISOFORM A-RELATED | 1 | 750 | 1.1E-132 |
| 5 | g11515.t1 | PANTHER | PTHR14030 | MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 | 1 | 750 | 1.1E-132 |
| 4 | g11515.t1 | PANTHER | PTHR14030:SF4 | BUB1 KINASE, ISOFORM A-RELATED | 825 | 1578 | 1.1E-132 |
| 6 | g11515.t1 | PANTHER | PTHR14030 | MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 | 825 | 1578 | 1.1E-132 |
| 1 | g11515.t1 | Pfam | PF08311 | Mad3/BUB1 homology region 1 | 36 | 157 | 1.8E-37 |
| 2 | g11515.t1 | Pfam | PF00069 | Protein kinase domain | 1299 | 1510 | 7.8E-20 |
| 12 | g11515.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 1422 | 1434 | - |
| 16 | g11515.t1 | ProSiteProfiles | PS51489 | BUB1 N-terminal domain profile. | 40 | 202 | 47.22 |
| 17 | g11515.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1298 | 1583 | 18.549 |
| 13 | g11515.t1 | SMART | SM00777 | mad3_bub1_i | 33 | 157 | 7.1E-48 |
| 14 | g11515.t1 | SMART | SM00220 | serkin_6 | 1298 | 1560 | 8.6E-10 |
| 7 | g11515.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1296 | 1560 | 2.32E-31 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0007094 | mitotic spindle assembly checkpoint signaling | BP |
KEGG
Orthology
- This transcript has been annotated with K02178 (checkpoint serine/threonine-protein kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
