General information for g11529.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ecdysone 20-monooxygenase.
Parent gene
- The parent gene g11529 is located on chr_1: 16878545..16886278 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11529 | g11529.t3 | isoform | g11529.t3 | 16873952 | 16878545 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon1 | 16873952 | 16874086 |
| chr_1 | g11529 | g11529.t3 | TSS | g11529.t3 | 16873955 | 16873955 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon2 | 16876464 | 16876719 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS1 | 16876482 | 16876719 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon3 | 16876785 | 16876895 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS2 | 16876785 | 16876895 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon4 | 16876954 | 16877367 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS3 | 16876954 | 16877367 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon5 | 16877417 | 16877626 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS4 | 16877417 | 16877626 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon6 | 16877692 | 16877873 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS5 | 16877692 | 16877873 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon7 | 16877936 | 16878013 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS6 | 16877936 | 16878013 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon8 | 16878074 | 16878231 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS7 | 16878074 | 16878231 |
| chr_1 | g11529 | g11529.t3 | exon | g11529.t3.exon9 | 16878380 | 16878545 |
| chr_1 | g11529 | g11529.t3 | cds | g11529.t3.CDS8 | 16878380 | 16878545 |
| chr_1 | g11529 | g11529.t3 | TTS | g11529.t3 | 16878795 | 16878795 |
Sequences
>g11529.t3 Gene=g11529 Length=1710
GTTTAGTCGATTCTTCCACCAGAACTTGAACGGTCAAGTTGCGAGAGACTCGCTAAATAA
ATTCTACATTAACCGCTGCCTAACTGTAAATTAATCCTAAAAATAAAAAATTAAATTGTG
ACATCAGAATCTTGGCAATTATTGTGATAAAAAATGGTGCCTTTAACCGTGATATTGTTG
TATGTTGCCATTGCATCGTTTCTAATCTACACATTCAAAAATAAGAAACCATTGAAATGG
AGTGAAATTGCCAATGCTATACCGTTTTACCAATACATTGTCAAAATCGACAGTGAAGAT
ACGTTTAAAATTCCTGGACCACTACGTCTTCCTTTCATTGGTACAAAATGGCAAAATATC
AAAATGAACAAACTACATGAATACTATGAAAGCCTGAACAACCTTTATGGAGATGTAGTA
TTGGAAGTATGCAGTAATAATGTTCCTGTGATTTCATTATTCAATAAAAATGACATTGAA
AAGGTTCTGCATTTCAATTCAGCTTATCCATTTCGTCCACCAACGGAAATTCTTTCTTAT
TATCGTAAAAGTCGACCCGATAGGTATGCTTCTTTGGGTTTAATTAACGAACAAGGACCG
GAGTGGGGAAGATTAAGAGCAAAAATGACTCCAAAAACCTTAGAAAGTCGAAAAGTACTT
TCTTCTTTCTGCTCTGACTTTAATGAACTTAGTGATGAATTTATAAATGTGATAAAACTT
AAACGAAATGAAGAAAATATTGTAAAAAATTTTGAAGAAACCATGAAATTGATGTCAATA
GAGGCTGCATGTGCATTAATTTTGGGAAGAAAGATGGGTTATTTAGCTGATAGCCATGAT
CATTTGGATAATAATTCAAAGGAGCTAATGATAGCTGTTAAAAATATTTTCGGAGTTATT
CGTGATTCATATTATGGTAATGGGATGTGGAAATATTTTCCAACGAAACTTTACAGTAAA
TACATTGAATATGAAGACAAAATTTATGATATAATTACGGGAATAATAAATGAAACTTTG
CATGACCATGAGTTTATGGCACTAGATAGTGATAATATGAGCATTCTAGCTATGATACTA
AAAGCTGATGGACTCGATATGAAAGATAAAATAAGTGGTGTAATTGACTTTTTAACTGCA
AGCCAAGTTTTGTCACACACGATGATTTTTTTACTTCATTTTCTATCTGAAAAGCCAGAA
GTTCAAGAAAAAATTTATCAAGAGACATTGCCATACATTAAAACACTTTCGTACGAAAAC
ATTAAAAAGTTCCATTACACACGTGCTGCCATTCTCGAGAGTCTTAGAATTTCACCAACA
GCATTTTCAGTTGCAAGAATTTTAGAACAAGATTTTAATTTTTCGGGTTACCACGTAAAA
GCTGGGAATATTGTCCTCTTGCAAAATATGGTTGCGTGCAACAAAGATGAGAATTTCGAG
AATGCCAAAACTTTCAATCCTGATAGATGGCTTAATGAAAATGGTGAATTCAATATAAAC
CAATGCTCTGCGTCACCTATAGTTATACCATTTGGATATGGGAAACGGGCATGTCCAGGA
AAAAAATTCTCTGAAATGGAGATGACAATCTTAACAGTTAAACTCATTCACTCGTACAAA
ATAGAATATCATTCAACATTTGAGAAGCAATTTGAGTTCCTTGTAGTACCTAAAGATCCA
GTTGACATAAAATTTACAAATCGATTCTGA
>g11529.t3 Gene=g11529 Length=518
MVPLTVILLYVAIASFLIYTFKNKKPLKWSEIANAIPFYQYIVKIDSEDTFKIPGPLRLP
FIGTKWQNIKMNKLHEYYESLNNLYGDVVLEVCSNNVPVISLFNKNDIEKVLHFNSAYPF
RPPTEILSYYRKSRPDRYASLGLINEQGPEWGRLRAKMTPKTLESRKVLSSFCSDFNELS
DEFINVIKLKRNEENIVKNFEETMKLMSIEAACALILGRKMGYLADSHDHLDNNSKELMI
AVKNIFGVIRDSYYGNGMWKYFPTKLYSKYIEYEDKIYDIITGIINETLHDHEFMALDSD
NMSILAMILKADGLDMKDKISGVIDFLTASQVLSHTMIFLLHFLSEKPEVQEKIYQETLP
YIKTLSYENIKKFHYTRAAILESLRISPTAFSVARILEQDFNFSGYHVKAGNIVLLQNMV
ACNKDENFENAKTFNPDRWLNENGEFNINQCSASPIVIPFGYGKRACPGKKFSEMEMTIL
TVKLIHSYKIEYHSTFEKQFEFLVVPKDPVDIKFTNRF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g11529.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 48 | 517 | 1.7E-88 |
| 2 | g11529.t3 | PANTHER | PTHR24305 | CYTOCHROME P450 | 49 | 511 | 9.8E-155 |
| 3 | g11529.t3 | PANTHER | PTHR24305:SF111 | ECDYSONE 20-MONOOXYGENASE | 49 | 511 | 9.8E-155 |
| 7 | g11529.t3 | PRINTS | PR00463 | E-class P450 group I signature | 337 | 363 | 4.5E-10 |
| 9 | g11529.t3 | PRINTS | PR00385 | P450 superfamily signature | 346 | 359 | 2.2E-8 |
| 8 | g11529.t3 | PRINTS | PR00385 | P450 superfamily signature | 378 | 389 | 2.2E-8 |
| 5 | g11529.t3 | PRINTS | PR00463 | E-class P450 group I signature | 417 | 441 | 4.5E-10 |
| 4 | g11529.t3 | PRINTS | PR00463 | E-class P450 group I signature | 457 | 467 | 4.5E-10 |
| 10 | g11529.t3 | PRINTS | PR00385 | P450 superfamily signature | 458 | 467 | 2.2E-8 |
| 6 | g11529.t3 | PRINTS | PR00463 | E-class P450 group I signature | 467 | 490 | 4.5E-10 |
| 11 | g11529.t3 | PRINTS | PR00385 | P450 superfamily signature | 467 | 478 | 2.2E-8 |
| 1 | g11529.t3 | Pfam | PF00067 | Cytochrome P450 | 54 | 514 | 2.1E-63 |
| 15 | g11529.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 16 | g11529.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 21 | - |
| 14 | g11529.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 22 | 518 | - |
| 18 | g11529.t3 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 460 | 469 | - |
| 12 | g11529.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 52 | 517 | 2.49E-70 |
| 17 | g11529.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K10723 (ecdysone 20-monooxygenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed