Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Ecdysone 20-monooxygenase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11529 g11529.t5 isoform g11529.t5 16876482 16877144
chr_1 g11529 g11529.t5 exon g11529.t5.exon1 16876482 16876719
chr_1 g11529 g11529.t5 cds g11529.t5.CDS1 16876482 16876719
chr_1 g11529 g11529.t5 exon g11529.t5.exon2 16876785 16876895
chr_1 g11529 g11529.t5 cds g11529.t5.CDS2 16876785 16876895
chr_1 g11529 g11529.t5 exon g11529.t5.exon3 16876954 16877144
chr_1 g11529 g11529.t5 cds g11529.t5.CDS3 16876954 16877144
chr_1 g11529 g11529.t5 TSS g11529.t5 NA NA
chr_1 g11529 g11529.t5 TTS g11529.t5 NA NA

Sequences

>g11529.t5 Gene=g11529 Length=540
ATGGTGCCTTTAACCGTGATATTGTTGTATGTTGCCATTGCATCGTTTCTAATCTACACA
TTCAAAAATAAGAAACCATTGAAATGGAGTGAAATTGCCAATGCTATACCGTTTTACCAA
TACATTGTCAAAATCGACAGTGAAGATACGTTTAAAATTCCTGGACCACTACGTCTTCCT
TTCATTGGTACAAAATGGCAAAATATCAAAATGAACAAACTACATGAATACTATGAAAGC
CTGAACAACCTTTATGGAGATGTAGTATTGGAAGTATGCAGTAATAATGTTCCTGTGATT
TCATTATTCAATAAAAATGACATTGAAAAGGTTCTGCATTTCAATTCAGCTTATCCATTT
CGTCCACCAACGGAAATTCTTTCTTATTATCGTAAAAGTCGACCCGATAGGTATGCTTCT
TTGGGTTTAATTAACGAACAAGGACCGGAGTGGGGAAGATTAAGAGCAAAAATGACTCCA
AAAACCTTAGAAAGTCGAAAAGTACTTTCTTCTTTCTGCTCTGACTTTAATGAACTTAGT

>g11529.t5 Gene=g11529 Length=180
MVPLTVILLYVAIASFLIYTFKNKKPLKWSEIANAIPFYQYIVKIDSEDTFKIPGPLRLP
FIGTKWQNIKMNKLHEYYESLNNLYGDVVLEVCSNNVPVISLFNKNDIEKVLHFNSAYPF
RPPTEILSYYRKSRPDRYASLGLINEQGPEWGRLRAKMTPKTLESRKVLSSFCSDFNELS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g11529.t5 Gene3D G3DSA:1.10.630.10 Cytochrome p450 48 180 7.0E-18
2 g11529.t5 PANTHER PTHR24305 CYTOCHROME P450 49 179 5.5E-46
3 g11529.t5 PANTHER PTHR24305:SF111 ECDYSONE 20-MONOOXYGENASE 49 179 5.5E-46
1 g11529.t5 Pfam PF00067 Cytochrome P450 54 167 3.6E-7
8 g11529.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 5 -
9 g11529.t5 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 6 21 -
7 g11529.t5 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 22 180 -
5 g11529.t5 SUPERFAMILY SSF48264 Cytochrome P450 52 167 1.83E-11
4 g11529.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 4 21 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed