General information for g1158.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase grp.
Parent gene
- The parent gene g1158 is located on chr_3: 8420732..8422262 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1158 | g1158.t2 | isoform | g1158.t2 | 8420732 | 8422262 |
| chr_3 | g1158 | g1158.t2 | exon | g1158.t2.exon1 | 8420732 | 8421569 |
| chr_3 | g1158 | g1158.t2 | cds | g1158.t2.CDS1 | 8420732 | 8421569 |
| chr_3 | g1158 | g1158.t2 | exon | g1158.t2.exon2 | 8421638 | 8421712 |
| chr_3 | g1158 | g1158.t2 | cds | g1158.t2.CDS2 | 8421638 | 8421712 |
| chr_3 | g1158 | g1158.t2 | exon | g1158.t2.exon3 | 8421773 | 8422262 |
| chr_3 | g1158 | g1158.t2 | cds | g1158.t2.CDS3 | 8421773 | 8422110 |
| chr_3 | g1158 | g1158.t2 | TSS | g1158.t2 | 8422454 | 8422454 |
| chr_3 | g1158 | g1158.t2 | TTS | g1158.t2 | NA | NA |
Sequences
>g1158.t2 Gene=g1158 Length=1403
ATGACTTACTCTCTTCCTTTGCCATATTCTATTAATAAAGATTGGTTGTTGCTTGAAAAA
CTTGGAGAAGGTGCATTTGCAAATGTCAGGTAAGAATAATAATATATCTACTGTAAAACT
TCAAATAAGCAAAAACATAACATTACAGACGAATGGTTTGTAAACGCACAAACAGTGCTT
ATGCCTTGAAATTGATTGATTTGAAAAAGAATACAAACACAGAAAAATCTGCCAAGAAAG
AGGCGTGCATTCTCAAGCTTTTATGTCATCCAAATATAGTTGAATTCTATCAAACATTCA
TGCTAAAACATGATGAACAAATCTTTCAATGTATCGTACTCGAGCTTATAACAGGAGGCG
AATTATTTCACCAAATTCCTCAAAATGTCGGCATTTCAATATCACAAGCGAGATTATATT
TTAATCAAATATTGGACGGTGTTGAATACATTCATTCTTGTGGGATTGCACATCGTGATC
TCAAACCTGAAAACATCTTAATCAGTGTGTCAAATAATTCTCCTTGCATAAAAATTTCTG
ATTTTGGAATGGCAACGGTATTTATGATTGATGGCAAACAACGTATGTTGAATAAAGAAT
GTGGATCACTTGCTTATATGGCACCAGAAGTAACTGAGAGTGCATATTTCGCTCAACCTG
CTGATATATGGTCAATTGGTATGATTTTAGTATTAATGCTAACGGGAACAAAACCATGGA
CAGAAGCAACCAAGTTCTCGACAAAATACATGAAATGGACAAAAAATGAAATTAGTGATA
TCAGTCCTTGGAATCGGCTTGATGAATACACACTATCTTTTCTACGAAAAATCCTCAATC
CATATCCAATGGAACGTTTGACCATACCACAAATAAAAGATGAGGATTGGTGTTGTAGTC
AATATATGAGTAATATTAATAAAATTTCATCAGTTCCTGTGGATAATACCATGATTGAAG
CTTTAACTCGGATGTGTAGTTTTATTGAAACAACACCATCTCCTATTCAGCAATATGAGC
AAAACTTCAACTTTAATATTCTTTCATCACCGCAATTAATAGAAGCACCTGAAGTCTATT
CAAAATTGTCTACAAATATTATTCGAACACCGTATGAAAAATATATAACAAGGTGTCTTG
TTAAAAGTGATGTATTTGAGACATTGAGACGATTAATAAGTGTATGTTCTAATTTTGAAT
GGACATGGAAAGTCAATGATTTTTCAATGACCGTATCTAGTCTTAATAGAGAGAATGTTA
CTTTTAAAATAAATATTTCTGAATTTAATGGAAATACTCTTATCGATATACGCTTATCAT
CGGGATGTGTTTTAGAATTCAAGCGTAACTTTTTCAAAATTAAGGAATCTAAGGAAATTC
TTCAAGTTCTTCTTCCAAGTTAA
>g1158.t2 Gene=g1158 Length=416
MVCKRTNSAYALKLIDLKKNTNTEKSAKKEACILKLLCHPNIVEFYQTFMLKHDEQIFQC
IVLELITGGELFHQIPQNVGISISQARLYFNQILDGVEYIHSCGIAHRDLKPENILISVS
NNSPCIKISDFGMATVFMIDGKQRMLNKECGSLAYMAPEVTESAYFAQPADIWSIGMILV
LMLTGTKPWTEATKFSTKYMKWTKNEISDISPWNRLDEYTLSFLRKILNPYPMERLTIPQ
IKDEDWCCSQYMSNINKISSVPVDNTMIEALTRMCSFIETTPSPIQQYEQNFNFNILSSP
QLIEAPEVYSKLSTNIIRTPYEKYITRCLVKSDVFETLRRLISVCSNFEWTWKVNDFSMT
VSSLNRENVTFKINISEFNGNTLIDIRLSSGCVLEFKRNFFKIKESKEILQVLLPS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g1158.t2 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 310 | 4.4E-68 |
| 2 | g1158.t2 | PANTHER | PTHR43895:SF116 | SERINE/THREONINE-PROTEIN KINASE CHK1 | 7 | 323 | 2.8E-67 |
| 3 | g1158.t2 | PANTHER | PTHR43895 | - | 7 | 323 | 2.8E-67 |
| 1 | g1158.t2 | Pfam | PF00069 | Protein kinase domain | 4 | 246 | 8.0E-58 |
| 6 | g1158.t2 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 105 | 117 | - |
| 8 | g1158.t2 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 252 | 37.714 |
| 5 | g1158.t2 | SMART | SM00220 | serkin_6 | 1 | 252 | 1.3E-53 |
| 4 | g1158.t2 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 3 | 290 | 6.2E-65 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
