General information for g11912.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4g15.
Parent gene
- The parent gene g11912 is located on chr_1: 19254806..19256607 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11912 | g11912.t1 | isoform | g11912.t1 | 19254806 | 19256607 |
| chr_1 | g11912 | g11912.t1 | exon | g11912.t1.exon1 | 19254806 | 19255375 |
| chr_1 | g11912 | g11912.t1 | cds | g11912.t1.CDS1 | 19254806 | 19255375 |
| chr_1 | g11912 | g11912.t1 | exon | g11912.t1.exon2 | 19255519 | 19256607 |
| chr_1 | g11912 | g11912.t1 | cds | g11912.t1.CDS2 | 19255519 | 19256607 |
| chr_1 | g11912 | g11912.t1 | TSS | g11912.t1 | NA | NA |
| chr_1 | g11912 | g11912.t1 | TTS | g11912.t1 | NA | NA |
Sequences
>g11912.t1 Gene=g11912 Length=1659
ATGAATAATACAGAAGTGGAAACAGTTGAAGCCACACAATGGAGTGTATTATCAATTACA
TCAATTTTTACACTCACAACTTTAGTTTTGCTTCATCTTTATCATATTAACACACACAAA
GCAAAATTAGGCAATAAAATACCAGGTCCAACAGCATTTCCTTTTATAGGGAATGCACTA
CTAATTCTTGGTCTTAAAAGCAACAATCATCTTTTTAGAAGAAGCAAAAAATTATCGGAT
AAATTTGGAAGAGTTGTTAGAGGATGGGCTGGTTGGAAATTGGTAGTTTTCATAATGAAT
CCAGCAGATGCTGAAGTGATTTTGAATAGCAGTGAACATATAGAGAAAAGTGATGAATAT
AAATTCTTTAAACCTTGGTTAGGCAATGGATTATTGATTTCTACTGGTGATAAATGGAGA
ACTCATAGAAAACTTATCGCACCAACTTTTCATATGAATATTCTCAAGAGTTTTGTGCCG
ACATTCAATGAAAAAAGTAAGAAAGTTGTGGAAAAGTTGAAATCGGTTGTGGGCATGGAA
ATTGATTGTCATGATTATATGAGTGAAACAACTGTTGATATTTTGCTTGAAACAGCAATG
GGATTTAAAAAGACTGACAAAGAGAAAGAAATGAGTGGATTTAAGTATGCTATGGCAGTA
ATGAGAATGTGCGATATTTTGCATGCACGACATGTTCGATTTTATTATCGCTTTAATTTA
ATTTTCAATTTATCAAAATATAAAAAAATACAAGATGAAGCTTTGAAAATTATTCATGGT
TTAACTGGAAAAGTCTTACAACAGAGACGAAAAGACTTTAATGAAAATTTACATAAAGGT
GAACTTCCAAGTCCATCAATTCAAGAAATAATTAAAGAAAATCCAAGAAAAGCAAATACA
ATTGATGGACTTCGTGATGATCTCGATGATATTGATGAAAATGATGTAGGAGAAAAACGA
AGATTGGCATTTTTAGATCTTATGATTGAGACATCACATTATAATCCAACTGAAATTTCT
GATGATGAGATTAAGCAGCAAGTGGATACAATCATGTTTGAGGGCCATGATACTTTGGAA
AGTTCCACAGCTGCAGGTTCAAGCTTTACACTGTGCATGCTCGGTTGTCATCCTGATATT
CAAGAGAAAGTGTATGCAGAACAGAAAGCAATATTTGGTAATTCTGATCGTGATTGTACC
TTTGCTGATACTTTAGAAATGAAATATCTAGAAAGAGTGATTTTTGAAACTCTGAGATTG
TATCCTCCAGTACCATTGATTGCTAGAAAAGTAAATGAAGATGTTAAGTTAGTAACTAAT
TGCTATGTGATTCCTGCTGGTACTACAGTATGCATTGGATTTTGGAAGTTACATAGAACA
CCAGAAAATTTTGAAAATCCCGACACATTTGATCCTGACAATTTTTTACCAGAAAGAACT
GCAAATCGTCATTATTACAGTTATATTCCTTTCTCAGCTGGACCTCGAAGTTGTGTCGGA
CGCAAATATGCGATGCTTAAATTAAAAATTCTTCTTAGTACTCTTGTTAGAAATTTTCAA
GTCAAATCATATTGCAAAGAAAATGAATTCAAAATGAGTGGTGATATAATTCTAAAACGA
GAAGATGGTTTTCGCATTGTTTTGGAAGCAAGAAATTGA
>g11912.t1 Gene=g11912 Length=552
MNNTEVETVEATQWSVLSITSIFTLTTLVLLHLYHINTHKAKLGNKIPGPTAFPFIGNAL
LILGLKSNNHLFRRSKKLSDKFGRVVRGWAGWKLVVFIMNPADAEVILNSSEHIEKSDEY
KFFKPWLGNGLLISTGDKWRTHRKLIAPTFHMNILKSFVPTFNEKSKKVVEKLKSVVGME
IDCHDYMSETTVDILLETAMGFKKTDKEKEMSGFKYAMAVMRMCDILHARHVRFYYRFNL
IFNLSKYKKIQDEALKIIHGLTGKVLQQRRKDFNENLHKGELPSPSIQEIIKENPRKANT
IDGLRDDLDDIDENDVGEKRRLAFLDLMIETSHYNPTEISDDEIKQQVDTIMFEGHDTLE
SSTAAGSSFTLCMLGCHPDIQEKVYAEQKAIFGNSDRDCTFADTLEMKYLERVIFETLRL
YPPVPLIARKVNEDVKLVTNCYVIPAGTTVCIGFWKLHRTPENFENPDTFDPDNFLPERT
ANRHYYSYIPFSAGPRSCVGRKYAMLKLKILLSTLVRNFQVKSYCKENEFKMSGDIILKR
EDGFRIVLEARN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g11912.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 30 | 552 | 3.7E-110 |
| 2 | g11912.t1 | PANTHER | PTHR24291:SF106 | CYTOCHROME P450 4G1-RELATED | 10 | 550 | 6.7E-240 |
| 3 | g11912.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 10 | 550 | 6.7E-240 |
| 8 | g11912.t1 | PRINTS | PR00463 | E-class P450 group I signature | 79 | 98 | 4.9E-23 |
| 6 | g11912.t1 | PRINTS | PR00463 | E-class P450 group I signature | 367 | 393 | 4.9E-23 |
| 10 | g11912.t1 | PRINTS | PR00385 | P450 superfamily signature | 376 | 389 | 2.8E-9 |
| 9 | g11912.t1 | PRINTS | PR00463 | E-class P450 group I signature | 411 | 429 | 4.9E-23 |
| 13 | g11912.t1 | PRINTS | PR00385 | P450 superfamily signature | 412 | 423 | 2.8E-9 |
| 5 | g11912.t1 | PRINTS | PR00463 | E-class P450 group I signature | 453 | 477 | 4.9E-23 |
| 4 | g11912.t1 | PRINTS | PR00463 | E-class P450 group I signature | 488 | 498 | 4.9E-23 |
| 12 | g11912.t1 | PRINTS | PR00385 | P450 superfamily signature | 489 | 498 | 2.8E-9 |
| 7 | g11912.t1 | PRINTS | PR00463 | E-class P450 group I signature | 498 | 521 | 4.9E-23 |
| 11 | g11912.t1 | PRINTS | PR00385 | P450 superfamily signature | 498 | 509 | 2.8E-9 |
| 1 | g11912.t1 | Pfam | PF00067 | Cytochrome P450 | 48 | 533 | 1.1E-103 |
| 17 | g11912.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 11 | - |
| 19 | g11912.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 35 | - |
| 16 | g11912.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 36 | 46 | - |
| 20 | g11912.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 47 | 65 | - |
| 18 | g11912.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 66 | 552 | - |
| 23 | g11912.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 491 | 500 | - |
| 14 | g11912.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 47 | 551 | 1.1E-108 |
| 22 | g11912.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 15 | 36 | - |
| 21 | g11912.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 43 | 65 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K05917 (sterol 14alpha-demethylase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed