Gene loci information

Transcript annotation

  • This transcript has been annotated as Vesicle transport protein USE1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1200 g1200.t1 TSS g1200.t1 8715762 8715762
chr_3 g1200 g1200.t1 isoform g1200.t1 8715821 8716576
chr_3 g1200 g1200.t1 exon g1200.t1.exon1 8715821 8715957
chr_3 g1200 g1200.t1 cds g1200.t1.CDS1 8715821 8715957
chr_3 g1200 g1200.t1 exon g1200.t1.exon2 8716018 8716576
chr_3 g1200 g1200.t1 cds g1200.t1.CDS2 8716018 8716576
chr_3 g1200 g1200.t1 TTS g1200.t1 8716734 8716734

Sequences

>g1200.t1 Gene=g1200 Length=696
ATGGCAGCAAAACTTGAAATAAATATAAGAAGTTTGTTAAGTACTTGTGAAGAGTTAGTA
AAAGAAGAAGATCAGAAATGGAAATTAACACGATATATAAAAAGCTTAGATTTAATGATC
AACGAATTGTCCGATTATGATACAGCAGATAAGAATCATATAGCAAAATACCAAGAAAAA
GTTCTAGAACTCAAAACACTTATTAATTATGTTGAACCACAAAAACAGAAAATAGTGAAA
ACTAGGAGGGATGTTGATGAGTCAGAAACAATTTTAAAGGAAATTAAGCAACTCAATAAC
GCAAAATTCAATAAAGAAATCAGAAAAGACTTATTTGATGGTGATGATGCAACAGGATTA
AGGAAAAGAGGGGCAAACGAAAGTTCAGAAAATATGGAAAGATATTATGATAATATTCAA
GAAAAATTAGGAGAAGAAATGCTTGCATTAACTAAAAATCTAAAAGAACAGTATTTAGCT
GCCAATAGCATAATTAAAAAAGATACAGAGGTCATAACTAAATCCGCAAAAATGGCTCAT
CAAAATATCGGATCACTAGAAAAAGAATCCAAAAAACTAAATGAGCATACAAAACGAGCA
TGTAAATGTTGGATGTGGATGATGATTGGTCTCGTAATTATGATATTTATCGCAATGGTC
CTATTCATGAAAATCATGAAGAAAAGGCAATATTAG

>g1200.t1 Gene=g1200 Length=231
MAAKLEINIRSLLSTCEELVKEEDQKWKLTRYIKSLDLMINELSDYDTADKNHIAKYQEK
VLELKTLINYVEPQKQKIVKTRRDVDESETILKEIKQLNNAKFNKEIRKDLFDGDDATGL
RKRGANESSENMERYYDNIQEKLGEEMLALTKNLKEQYLAANSIIKKDTEVITKSAKMAH
QNIGSLEKESKKLNEHTKRACKCWMWMMIGLVIMIFIAMVLFMKIMKKRQY

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g1200.t1 CDD cd15860 SNARE_USE1 136 195 5.31412E-15
5 g1200.t1 Coils Coil Coil 2 22 -
2 g1200.t1 PANTHER PTHR13050 USE1-LIKE PROTEIN 1 229 2.3E-29
3 g1200.t1 PANTHER PTHR13050:SF7 VESICLE TRANSPORT PROTEIN USE1 1 229 2.3E-29
1 g1200.t1 Pfam PF09753 Membrane fusion protein Use1 4 226 9.6E-67
7 g1200.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 203 -
8 g1200.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 204 223 -
6 g1200.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 224 231 -
4 g1200.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 204 223 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values