Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Vesicle transport protein USE1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1200 g1200.t10 TSS g1200.t10 8715762 8715762
chr_3 g1200 g1200.t10 isoform g1200.t10 8715821 8716576
chr_3 g1200 g1200.t10 exon g1200.t10.exon1 8715821 8715957
chr_3 g1200 g1200.t10 exon g1200.t10.exon2 8716026 8716576
chr_3 g1200 g1200.t10 cds g1200.t10.CDS1 8716274 8716576
chr_3 g1200 g1200.t10 TTS g1200.t10 8716734 8716734

Sequences

>g1200.t10 Gene=g1200 Length=688
ATGGCAGCAAAACTTGAAATAAATATAAGAAGTTTGTTAAGTACTTGTGAAGAGTTAGTA
AAAGAAGAAGATCAGAAATGGAAATTAACACGATATATAAAAAGCTTAGATTTAATGATC
AACGAATTGTCCGATTACAGATAAGAATCATATAGCAAAATACCAAGAAAAAGTTCTAGA
ACTCAAAACACTTATTAATTATGTTGAACCACAAAAACAGAAAATAGTGAAAACTAGGAG
GGATGTTGATGAGTCAGAAACAATTTTAAAGGAAATTAAGCAACTCAATAACGCAAAATT
CAATAAAGAAATCAGAAAAGACTTATTTGATGGTGATGATGCAACAGGATTAAGGAAAAG
AGGGGCAAACGAAAGTTCAGAAAATATGGAAAGATATTATGATAATATTCAAGAAAAATT
AGGAGAAGAAATGCTTGCATTAACTAAAAATCTAAAAGAACAGTATTTAGCTGCCAATAG
CATAATTAAAAAAGATACAGAGGTCATAACTAAATCCGCAAAAATGGCTCATCAAAATAT
CGGATCACTAGAAAAAGAATCCAAAAAACTAAATGAGCATACAAAACGAGCATGTAAATG
TTGGATGTGGATGATGATTGGTCTCGTAATTATGATATTTATCGCAATGGTCCTATTCAT
GAAAATCATGAAGAAAAGGCAATATTAG

>g1200.t10 Gene=g1200 Length=100
MERYYDNIQEKLGEEMLALTKNLKEQYLAANSIIKKDTEVITKSAKMAHQNIGSLEKESK
KLNEHTKRACKCWMWMMIGLVIMIFIAMVLFMKIMKKRQY

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g1200.t10 CDD cd15860 SNARE_USE1 5 64 1.4068E-17
2 g1200.t10 PANTHER PTHR13050 USE1-LIKE PROTEIN 7 98 8.1E-24
3 g1200.t10 PANTHER PTHR13050:SF7 VESICLE TRANSPORT PROTEIN USE1 7 98 8.1E-24
1 g1200.t10 Pfam PF09753 Membrane fusion protein Use1 3 95 4.9E-41
6 g1200.t10 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 72 -
7 g1200.t10 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 73 92 -
5 g1200.t10 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 93 100 -
4 g1200.t10 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 73 95 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values